Re: [Histonet] Xylene substitutes for clearing agents

2019-08-28 Thread Rene J Buesa via Histonet 
 Under separate cover I am sending you 3 papers of mine that answer your 
question.René
On Wednesday, August 28, 2019, 08:45:31 AM EDT, Ingles Claire via Histonet 
 wrote:  
 
 Chris:
Propar from Anatech works great for us. I believe it is still advisable to use 
xylene in the cleaning cycle on the processors though. We had to go back the 
other way a bit when our Doc wanted a tape coverslipper. Now he gripes about 
the xylene smell. Hmmm.
Claire


From: Hagon, Christopher (Health) via Histonet 

Sent: Tuesday, August 27, 2019 10:53 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Xylene substitutes for clearing agents

WARNING: This email appears to have originated outside of the UW Health email 
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UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the 
comparisons of each. I am charged with looking into converting our lab to go 
xylene free. We don't want to go down the limonene path, so that leaves the 
isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution 
(Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to 
change procedures. Was there much trial and error in changing the processing 
protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au<https://health.act.gov.au/>



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Re: [Histonet] Xylene substitutes for clearing agents

2019-08-28 Thread Ingles Claire via Histonet 
Chris:
Propar from Anatech works great for us. I believe it is still advisable to use 
xylene in the cleaning cycle on the processors though. We had to go back the 
other way a bit when our Doc wanted a tape coverslipper. Now he gripes about 
the xylene smell. Hmmm.
Claire


From: Hagon, Christopher (Health) via Histonet 

Sent: Tuesday, August 27, 2019 10:53 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Xylene substitutes for clearing agents

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.




UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the 
comparisons of each. I am charged with looking into converting our lab to go 
xylene free. We don't want to go down the limonene path, so that leaves the 
isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution 
(Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to 
change procedures. Was there much trial and error in changing the processing 
protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au<https://health.act.gov.au/>



---
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[Histonet] Xylene substitutes for clearing agents

2019-08-27 Thread Hagon, Christopher (Health) via Histonet 
UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the 
comparisons of each. I am charged with looking into converting our lab to go 
xylene free. We don't want to go down the limonene path, so that leaves the 
isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution 
(Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to 
change procedures. Was there much trial and error in changing the processing 
protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au



---
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you are not the intended recipient, please notify the sender and delete all 
copies of this transmission along with any attachments immediately. You should 
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person.
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