[Histonet] Antigen retrieval question. and a bit more
Hello 'netters, hope you all had a good weekend. We are having some difficulties with the pressure cooker chambers we use for antigen retrieval. What different methodologies would be recommended? If we went to a different methodology for retrieval, would each antigen have to be revalidated? How many slides/runs would you recommend? Thanks. For any interested, new blog post http://www.chicagonow.com/downsize-maybe/2016/01/why-did-my-cat-dump-me/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] antigen retrieval question
Lynn, I use the Decloaker and also stain for BVD, so will get in contact with you later this afternoon. Jan Shivers University of Minnesota Veterinary Diagnostic Lab St. Paul, MN 55108 On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn wrote: > If anyone out there is using a decloaker for antigen retrieval, when doing > ihc staining, would you please contact me on my e-mail address? I have been > struggling with a BVD test for months. It is not working consistently and I > want to explore using this method along with our Benchmark XT stainer from > Ventana. > Thank you, > Lynn Burton > Galesburg Animal Disease Lab > Galesburg, Il > lynn.bur...@illinois.gov > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] antigen retrieval question
Is this with an older tissue sample? We don't do antigen retrieval, but we do have a proprietary technique for tissue restoration which can help with problematic IHC studies, especially in older FFPE tissue samples. See here for some examples: http://hematoxylin-eosin-tales.blogspot.com/2013/04/new-technique-for-improving-staining-in.html We could treat your sections for free if you like. Let me know if you are interested. Rob Day. On May 30, 2013, at 10:54 AM, "Burton, Lynn" wrote: > If anyone out there is using a decloaker for antigen retrieval, when doing > ihc staining, would you please contact me on my e-mail address? I have been > struggling with a BVD test for months. It is not working consistently and I > want to explore using this method along with our Benchmark XT stainer from > Ventana. > Thank you, > Lynn Burton > Galesburg Animal Disease Lab > Galesburg, Il > lynn.bur...@illinois.gov > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] antigen retrieval question
If anyone out there is using a decloaker for antigen retrieval, when doing ihc staining, would you please contact me on my e-mail address? I have been struggling with a BVD test for months. It is not working consistently and I want to explore using this method along with our Benchmark XT stainer from Ventana. Thank you, Lynn Burton Galesburg Animal Disease Lab Galesburg, Il lynn.bur...@illinois.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antigen retrieval question
Consider the following sequence:
1- the NBF corsslinked the antigens making them "more" stable, "insolubable".
2- you did HIER and that crosslinkage disappeared, making the antigen
"vulnerable" or able to be dissolved
3- you left them in buffer while "vulnerable". Does not sound to you as if the
antigens were "diluted", "dissolved" in the buffer causing a weaker reaction
than usual?
That would be my explanation, but to your original question ("Have you
experienced...") my answer is no, I always proceeded with the IHC test
immediately after HIER. Maybe that is why it has not happened to me.
Just a thought!
René J.
--- On Tue, 10/20/09, Connolly, Brett M wrote:
From: Connolly, Brett M
Subject: [Histonet] Antigen retrieval question
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 20, 2009, 2:21 PM
Has anyone experienced a "reversal" of citrate HEIR unmasking do to a
prolonged delay in continuing the experiment after the retrieval step?
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_conno...@merck.com
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Re: [Histonet] Antigen retrieval question
Sounds like the retrieved antigen was lost rather than "reversal" Janet QIHC/HTL --- On Tue, 10/20/09, Connolly, Brett M wrote: From: Connolly, Brett M Subject: [Histonet] Antigen retrieval question To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 20, 2009, 11:21 AM Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antigen retrieval question
Has anyone experienced a "reversal" of citrate HEIR unmasking do to a prolonged delay in continuing the experiment after the retrieval step? Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
