Anything using FITC is pretty expensive. If you aren't want= ing
flourescence then why would you use this? Does this specific conj
ugation eliminate the Fc binding? Conjugating to HRP doesn't seem to
= yield that Fc region staining that I can see? Just a happy Monday
que= stion. =)
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
<= /span>
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 = Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: Re: [Histonet] human on human IHC
From: [1]andreahoo...@rocketm= ail.com
Date: Mon, July 19, 2010 6:06 am
To: [2]sgoe...@xbiotech.com, "Ki= m Merriam" <[3]kmerriam2...@yah
oo.com>
Cc: "Histonet" <[4]h= isto...@lists.utsouthwestern.edu>
Along those same lines I have conjugated primaries to biotin and it
works b= eautifully for human on human IHC. Another alternative if
extra amplificati= on is needed is to conjugating to FITC and then
using a rabbit-anti-FITC fo= llowed by an anti-rabbit polymer.
Just be aware with human on human controls are imperative as you may
get bi= nding to human Fc receptors.
Andrea Hooper
Sent from my Verizon Wireless BlackBerry
-Original Message-
From: <[5]sgoe...@xbiotech.com>
Sender: [6]hist= onet-boun...@lists.utsouthwestern.edu
Date: Thu, 15 Jul 2010 09:27:19
To: Kim Merriam<[7]kmerriam2003= @yahoo.com>
Cc: Histonet<[8]hist= o...@lists.utsouthwestern.edu>
Subject: RE: [Histonet] human on human IHC
I am doing alot with this right now. The best thing I havefound to
do is to conjugate your primary antibody with HRP, then just use
the direct method (no secondary), and it turns out beautifully!!
You can buy thekit to do this from Fisher. I can get the exact
order number if you need it. Good Luck!!
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
---- Original Message
Subject: [Histonet] human on human IHC
From: Kim Merriam <[1][9]kme= rriam2...@yahoo.com>
Date: Thu, July 15, 2010 8:00 am
To: Histonet <[2][10]histo...@lists.utsouthwestern.edu>
Hi,
Does anyone know of a human-on-human IHC kit similar to the
mouse-on-mouse kits
that are available?
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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