AW: [Histonet] moleculer testing
Hi, this histolab does BRAF, KRAS, EGFR with pyrosequencing and pcr; her2-amplification with DDISH on Ventana-Benchmark and ALK with FISH. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cynthia Pyse Gesendet: Montag, 10. Dezember 2012 15:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] moleculer testing Happy Monday Histonetter Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and HER2neu and what testing methods are you using. I prefer not to use FISH. Would rather have a permanent record of the slides. Any information is welcome. Have a great week. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] moleculer testing
Hi Cynthia, We do PCR for KRAS and BRAF. You cant do these by FISH anyway, the mutations are too small. We do FISH for ALK and HER2. For ALK, there is no antibody that can detect all the positive cases. ALK FISH is the only method that can detect ALK break aparts regardless of the fusion partner for ALK. If you want to do PCR for ALK, you will most likely be testing mRNA which isnt too stable and since all the ALK mutations are not known, you will miss some. This could require more tissue too. For HER2 there are some chromogenic ISH methods. I still like FISH since you can look at each color chanel individually. I never worry that one color is hiding the other and you can see each signal (no estimation of # of signals in a cluster). I dont think having a permanent record on a slide is the most important thing for molecular testing. Mark On Monday, December 10, 2012, Cynthia Pyse wrote: > Happy Monday Histonetter > > Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and > HER2neu and what testing methods are you using. I prefer not to use FISH. > Would rather have a permanent record of the slides. Any information is > welcome. Have a great week. > > Cindy > > > > Cindy Pyse, CLT, HT (ASCP) > > Laboratory Manager > > X-Cell Laboratories > > 20 Northpointe Parkway Suite 100 > > Amherst, NY 14228 > > 716-250-9235 etx. 232 > > e-mail cp...@x-celllab.com > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] moleculer testing
Happy Monday Histonetter Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and HER2neu and what testing methods are you using. I prefer not to use FISH. Would rather have a permanent record of the slides. Any information is welcome. Have a great week. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet