Re: [Histonet] need a counter stain for esterase and silver stained slides

2011-06-22 Thread Rene J Buesa 
Why don't you try immersing the sections for 1-2 seconds in saturated aqueous 
picric acid?
René J.

--- On Wed, 6/22/11, Nicole Cosenza  wrote:


From: Nicole Cosenza 
Subject: [Histonet] need a counter stain for esterase and silver stained slides
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, June 22, 2011, 3:05 PM


I have recently stained some muscle tissue with bromoindigo and urea-silver to 
examine the neuromuscular junction.  The nerve axons and endplates stain quite 
well this way, but I'd like to add some color to the slide for 
picture-purposes. Is there a good counterstain (eosin, or Biebrich scarlet-acid 
fuchsin) I can dip the slides in to add color, WITHOUT taking away from the 
stain already present in the slides?

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[Histonet] need a counter stain for esterase and silver stained slides

2011-06-22 Thread Nicole Cosenza 
I have recently stained some muscle tissue with bromoindigo and 
urea-silver to examine the neuromuscular junction.  The nerve axons and 
endplates stain quite well this way, but I'd like to add some color to 
the slide for picture-purposes. Is there a good counterstain (eosin, or 
Biebrich scarlet-acid fuchsin) I can dip the slides in to add color, 
WITHOUT taking away from the stain already present in the slides?


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