Re: [Histonet] questionaire about fixation
As long as you a line the tissue is NOT EVEN penetrated and much less fixed. René J. --- On Mon, 3/19/12, Emily Sours wrote: From: Emily Sours Subject: Re: [Histonet] questionaire about fixation To: histonet@lists.utsouthwestern.edu Date: Monday, March 19, 2012, 12:29 PM So if you have a line, doesn't that mean your tissue isn't fixed properly? The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson On Mon, Mar 19, 2012 at 12:26 PM, Rene J Buesa wrote: > Emily: > With formalin fixation, the formalin advances within the tissue in a way > that you can macroscopically see up to where the formalin has penetrated. > The appearance of the tissue changes in color and consistence. The change > in appearance goes from the exterior to the interior of the tissue and that > advance is seen as a line, hence "the front line" of the penetration. > René J. > > --- On *Sun, 3/18/12, Emily Sours * wrote: > > > From: Emily Sours > Subject: Re: [Histonet] questionaire about fixation > To: histonet@lists.utsouthwestern.edu > Date: Sunday, March 18, 2012, 10:23 PM > > > What the heck is a frontline? > I tried to google it, but I got nothing useful. > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that’s beautiful. > --Ron Swanson > > > > On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang > http://us.mc1621.mail.yahoo.com/mc/compose?to=gu.l...@gmx.at>> > wrote: > > > Dear histonet! > > > > A few hours after submersion of a tissue-block (eg liver) in NBF, the > block > > is cut across and we see the colour-change from brown to grey at the > > margins > > until a frontline. > > > > This frontline shows us: > > > > a) the distance of penetration of NBF; > > > > b) the distance of penetration AND addition of methylol-groups; > > > > c) the distance of penetration AND addition of methylol-groups AND (at > > least > > beginning) cross-linking. > > > > > > > > Do you see the issue? > > > > I would be happy, if you can help me to find a correct answer. > > > > > > > > Regards > > > > Gudrun Lang > > > > > > > > Biomed. Analytikerin > > > > Histolab Linz, Austria > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu<http://us.mc1621.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<http://us.mc1621.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] questionaire about fixation
So if you have a line, doesn't that mean your tissue isn't fixed properly? The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson On Mon, Mar 19, 2012 at 12:26 PM, Rene J Buesa wrote: > Emily: > With formalin fixation, the formalin advances within the tissue in a way > that you can macroscopically see up to where the formalin has penetrated. > The appearance of the tissue changes in color and consistence. The change > in appearance goes from the exterior to the interior of the tissue and that > advance is seen as a line, hence "the front line" of the penetration. > René J. > > --- On *Sun, 3/18/12, Emily Sours * wrote: > > > From: Emily Sours > Subject: Re: [Histonet] questionaire about fixation > To: histonet@lists.utsouthwestern.edu > Date: Sunday, March 18, 2012, 10:23 PM > > > What the heck is a frontline? > I tried to google it, but I got nothing useful. > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that’s beautiful. > --Ron Swanson > > > > On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang > http://us.mc1621.mail.yahoo.com/mc/compose?to=gu.l...@gmx.at>> > wrote: > > > Dear histonet! > > > > A few hours after submersion of a tissue-block (eg liver) in NBF, the > block > > is cut across and we see the colour-change from brown to grey at the > > margins > > until a frontline. > > > > This frontline shows us: > > > > a) the distance of penetration of NBF; > > > > b) the distance of penetration AND addition of methylol-groups; > > > > c) the distance of penetration AND addition of methylol-groups AND (at > > least > > beginning) cross-linking. > > > > > > > > Do you see the issue? > > > > I would be happy, if you can help me to find a correct answer. > > > > > > > > Regards > > > > Gudrun Lang > > > > > > > > Biomed. Analytikerin > > > > Histolab Linz, Austria > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu<http://us.mc1621.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<http://us.mc1621.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] questionaire about fixation
Emily: With formalin fixation, the formalin advances within the tissue in a way that you can macroscopically see up to where the formalin has penetrated. The appearance of the tissue changes in color and consistence. The change in appearance goes from the exterior to the interior of the tissue and that advance is seen as a line, hence "the front line" of the penetration. René J. --- On Sun, 3/18/12, Emily Sours wrote: From: Emily Sours Subject: Re: [Histonet] questionaire about fixation To: histonet@lists.utsouthwestern.edu Date: Sunday, March 18, 2012, 10:23 PM What the heck is a frontline? I tried to google it, but I got nothing useful. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang wrote: > Dear histonet! > > A few hours after submersion of a tissue-block (eg liver) in NBF, the block > is cut across and we see the colour-change from brown to grey at the > margins > until a frontline. > > This frontline shows us: > > a) the distance of penetration of NBF; > > b) the distance of penetration AND addition of methylol-groups; > > c) the distance of penetration AND addition of methylol-groups AND (at > least > beginning) cross-linking. > > > > Do you see the issue? > > I would be happy, if you can help me to find a correct answer. > > > > Regards > > Gudrun Lang > > > > Biomed. Analytikerin > > Histolab Linz, Austria > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] questionaire about fixation
What the heck is a frontline? I tried to google it, but I got nothing useful. The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang wrote: > Dear histonet! > > A few hours after submersion of a tissue-block (eg liver) in NBF, the block > is cut across and we see the colour-change from brown to grey at the > margins > until a frontline. > > This frontline shows us: > > a) the distance of penetration of NBF; > > b) the distance of penetration AND addition of methylol-groups; > > c) the distance of penetration AND addition of methylol-groups AND (at > least > beginning) cross-linking. > > > > Do you see the issue? > > I would be happy, if you can help me to find a correct answer. > > > > Regards > > Gudrun Lang > > > > Biomed. Analytikerin > > Histolab Linz, Austria > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] questionaire about fixation
Dear histonet! A few hours after submersion of a tissue-block (eg liver) in NBF, the block is cut across and we see the colour-change from brown to grey at the margins until a frontline. This frontline shows us: a) the distance of penetration of NBF; b) the distance of penetration AND addition of methylol-groups; c) the distance of penetration AND addition of methylol-groups AND (at least beginning) cross-linking. Do you see the issue? I would be happy, if you can help me to find a correct answer. Regards Gudrun Lang Biomed. Analytikerin Histolab Linz, Austria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
