Re: [Histonet] Acceptable way to "bake" sections onto slides?
Hi Everyone Just for clarification; our current practice involves a mixture of placing racks of slides in an oven for 25 minutes, or using the hot area of an autostainer (we're talking H&E's here, BTW). Occasionally, with a particularly urgent case us old-timers will place individual slides directly onto the floor of an oven for 5 mins which bakes the sections onto the slides very nicely prior to staining. My theory is that a 60-70 celsius hotplate will perform the same job for all of our H&E slides. So far, I haven't noticed any particular artefacts, and we don't suffer from water pooling between section and slide anyway. I'm trying to find both the "leanest" and cheap (hey - this is the NHS!) solution by consolidating four stainers and three coverslippers into just the two machines. Maybe I've not looked closely enough at our super-urgent H&E's... Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Acceptable way to "bake" sections onto slides?
Totally agree with Rene. You will need to use some type of drying oven Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 13, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu; PhilipGibson Subject: Re: [Histonet] Acceptable way to "bake" sections onto slides? The correct answer is "b": if there is water underneath the section that is going to be placed on the hot plate, the most likely artifact is that of "empty nuclei" that will ruin the sections' usefulness. René J. --- On Fri, 8/13/10, Gibson, Philip wrote: From: Gibson, Philip Subject: [Histonet] Acceptable way to "bake" sections onto slides? To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 8:23 AM Hi In our "fairly large" histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to "bake" for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Acceptable way to "bake" sections onto slides?
The correct answer is "b": if there is water underneath the section that is going to be placed on the hot plate, the most likely artifact is that of "empty nuclei" that will ruin the sections' usefulness. René J. --- On Fri, 8/13/10, Gibson, Philip wrote: From: Gibson, Philip Subject: [Histonet] Acceptable way to "bake" sections onto slides? To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 8:23 AM Hi In our "fairly large" histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to "bake" for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Acceptable way to "bake" sections onto slides?
Use the sakura DRS hotpot Loading from the plate (apart for the other drawbacks) will set the wax on the first slides put in the racks and you may end uo with inadequate dewaxing Just my 5cents worth Annieinarabia Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: "Gibson, Philip" Sender: histonet-boun...@lists.utsouthwestern.edu Date: Fri, 13 Aug 2010 13:23:34 To: Subject: [Histonet] Acceptable way to "bake" sections onto slides? Hi In our "fairly large" histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to "bake" for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
