RE: [Histonet] Biocare P63 with Dako Flex detection

[Cynthia Pyse]

Cathy
Have you tried adding an EnVision FLEX mouse linker to your protocol.
Sometimes that will work.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
cathy.crump...@tuality.org
Sent: Wednesday, July 21, 2010 3:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biocare P63 with Dako Flex detection


   Hi  all, I have ran into a troubleshooting dilemma and was wonderi=g
   if  anyone  else could help.  Is there anyone out there that uses the
Biocare  Medical  P63 concentrated antibody with the Dako TRS and Flex
   detec=tion  kit?  I am curious what dilution you use and if you have
   to  do  an=ything  special  for  the  P63 (extra long retrieval, DAB
   enhancer, etc). = am having problems getting a strong signal for the
   basal cells in prosta=e.  I am running it at 1:50 with 40 minutes in
   pH  9  retrieval  (which  =seems  too  long to me but does help).  A
   dilution with the same protoco= at 1:100 is way too light.

   Thanks,
<=DIV>
   Cathy Crumpton HT(ASCP), Histology Lead
   Tuality Community Hosp=tal
   Hillsboro, OR 97123
   (503)681-1292
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Re: [Histonet] Biocare P63 with Dako Flex detection

[Mark Tarango]

Hi Cathy,

I'm using their p63, but on the Ventana XT with ultraview DAB detection.  We
use it at 1:200 and get a strong signal in both breast and prostate
tissues.

If you haven't tried staining various cases of prostate, I'd suggest it.
Sometimes I'll get a prostate that stains the basal cells nicely and other
times the signal is much weaker.  It might have something to do with
fixation in the resection specimens.  If you can't get a strong signal in a
prostate needle biopsy, then it's definately a staining issue and not the
tissue.

I got stuck trying to stain a bad prostate once and wouldn't want that to
happen to you.

Good Luck!

Mark

On Wed, Jul 21, 2010 at 12:46 PM,  wrote:

>
>   Hi  all, I have ran into a troubleshooting dilemma and was wonderi ng
>   if  anyone  else could help.  Is there anyone out there that uses the
> Biocare  Medical  P63 concentrated antibody with the Dako TRS and Flex
>   detec  tion  kit?  I am curious what dilution you use and if you have
>   to  do  an  ything  special  for  the  P63 (extra long retrieval, DAB
>   enhancer, etc).  I am having problems getting a strong signal for the
>   basal cells in prosta te.  I am running it at 1:50 with 40 minutes in
>   pH  9  retrieval  (whichseems  too  long to me but does help).  A
>   dilution with the same protoco l at 1:100 is way too light.
>
>   Thanks,
>< /DIV>
>   Cathy Crumpton HT(ASCP), Histology Lead
>   Tuality Community Hosp ital
>   Hillsboro, OR 97123
>   (503)681-1292
>___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
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