RE: [Histonet] Embeeding frozen tissue in paraffin
No you need to thaw/fix in formalin or alcoholic fixative in order to process and embed in paraffin. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pru...@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, May 15, 2009 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Embeeding frozen tissue in paraffin I assume you mean the tissue was frozen without fixation, not that you want to put it into paraffin without fixation? No tissue will withstand paraffin processing without fixation. The best way to turn a frozen tissue into a paraffin embedded tissue is to drop the frozen tissue (either plain or in embedding medium, whichever is the case) into formalin while still frozen. Let it thaw and fix in the formalin, using a typical fixation time for a tissue that size, then just process and embed it as you would any tissue. > -- > From: histonet-boun...@lists.utsouthwestern.edu on behalf of Tomasz Bonda > Sent: Friday, May 15, 2009 6:19 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embeeding frozen tissue in paraffin > > Hello, > is there any good method for embeeding fresh frozen tissue (without any > fixation) in paraffin? > I would be grateful for ANY suggestions. > > T. Bonda > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embeeding frozen tissue in paraffin
I assume you mean the tissue was frozen without fixation, not that you want to put it into paraffin without fixation? No tissue will withstand paraffin processing without fixation. The best way to turn a frozen tissue into a paraffin embedded tissue is to drop the frozen tissue (either plain or in embedding medium, whichever is the case) into formalin while still frozen. Let it thaw and fix in the formalin, using a typical fixation time for a tissue that size, then just process and embed it as you would any tissue. > -- > From: histonet-boun...@lists.utsouthwestern.edu on behalf of Tomasz > Bonda > Sent: Friday, May 15, 2009 6:19 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embeeding frozen tissue in paraffin > > Hello, > is there any good method for embeeding fresh frozen tissue (without any > fixation) in paraffin? > I would be grateful for ANY suggestions. > > T. Bonda > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embeeding frozen tissue in paraffin
> is there any good method for embeeding fresh frozen tissue (without any fixation) in paraffin? Wouldn't the molten paraffin would melt the frozen tissue? LOL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embeeding frozen tissue in paraffin
why would you want to do this? - the water contained in the fresh tissue will not "mix" with the paraffin wax. There would be little purpose in trying. On 5/15/09, Tomasz Bonda wrote: > > Hello, > is there any good method for embeeding fresh frozen tissue (without any > fixation) in paraffin? > I would be grateful for ANY suggestions. > > T. Bonda > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
