RE: [Histonet] Gastric biopsies
Jennifer, I read my response and realize I made the mistake of stating on all esophageal biopsies. We only do candida when the Endoscopy physicians list it on the paperwork. I ran out of our control for fungus so I am purchasing controls from American Master Tech. (they use superfrost plus slides which is what Ventana approves for our NexES stainer) The American Master Tech fungus controls stain well with Pas light green and GMS. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cl...@wchsys.org From: Schumacher, Jennifer J [jschu...@fairview.org] Sent: Thursday, May 13, 2010 10:38 AM To: Joyce Cline Subject: RE: [Histonet] Gastric biopsies Joyce, I am facinated by your routine PAS/light green on esophogeal biopsies. We are talking about working this stain up in house, but have had trouble finding candida control tissue. May I ask what you use or how you obtain your controls? Jennifer From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline [joyce.cl...@wchsys.org] Sent: Thursday, May 13, 2010 8:44 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gastric biopsies We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies if requested by the grossing pathologist. We do Diff-Quik on all stomach biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done on colon with a diagnosis of microscopic or collagenous colitis . A pas light green is done on esophogeal biopsies for candida. Any other specials are done at the request of the pathologist. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson [cls71...@sbcglobal.net] Sent: Wednesday, May 12, 2010 5:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastric biopsies Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gastric biopsies
We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies if requested by the grossing pathologist. We do Diff-Quik on all stomach biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done on colon with a diagnosis of microscopic or collagenous colitis . A pas light green is done on esophogeal biopsies for candida. Any other specials are done at the request of the pathologist. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson [cls71...@sbcglobal.net] Sent: Wednesday, May 12, 2010 5:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastric biopsies Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gastric biopsies
For H. pylori we do a Genta, which is a modified Steiner, with Alcian blue and H & E over it. I've finally gotten the docs to wait to make sure they need specials on everything else, so as not to waste. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susanfpl...@aim.com Sent: Wednesday, May 12, 2010 21:57 To: cls71...@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gastric biopsies We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies. Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori. We occasionally did H. Pylori using immunohistochemistry. We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies. Hope this helps. Susan Plaza, HT (ASCP), QIHC -Original Message- From: Cristi stephenson To: histonet Sent: Wed, May 12, 2010 5:25 pm Subject: [Histonet] Gastric biopsies Hello Histoland, was wondering if anyone out there does stains other than H&E 's on specific issues. For example, does anyone run stains to rule out H. pylori on all astric biopsies? Any information as to the standards in other labs would be reat. hanks, risti __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Gastric biopsies
For gastric biopsies we routinely did modified Steiner for H. pylori René J. --- On Wed, 5/12/10, Cristi stephenson wrote: From: Cristi stephenson Subject: [Histonet] Gastric biopsies To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 12, 2010, 5:57 PM Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gastric biopsies
Christi We run a diff stain on all gastric bx suspected of H. pylori as routine, it saves us time. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Wednesday, May 12, 2010 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastric biopsies Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gastric biopsies
Hello Cristi, We routinely do a steiner on all Gastric Bx.'s we also do Pas W and WO digestion, Fe, Trichrome and retic on all liver with DX of Hepatitis. GMS-P on Lung bx POS. for HIV if requested by Dr. Elastic, retic and Trichrome on Temporal Arteries. Caula Gill (HT) ASCP -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Wednesday, May 12, 2010 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastric biopsies Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Gastric biopsies
We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies. Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori. We occasionally did H. Pylori using immunohistochemistry. We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies. Hope this helps. Susan Plaza, HT (ASCP), QIHC -Original Message- From: Cristi stephenson To: histonet Sent: Wed, May 12, 2010 5:25 pm Subject: [Histonet] Gastric biopsies Hello Histoland, was wondering if anyone out there does stains other than H&E 's on specific issues. For example, does anyone run stains to rule out H. pylori on all astric biopsies? Any information as to the standards in other labs would be reat. hanks, risti __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Gastric biopsies
We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all esophageal biopsies with Barrett's indicated by the GI Dr. Our other special stains are by request, but we almost always end up doing retic and trichrome on liver bx submitted with hepatitis as Clinical Dx. Hope this helps. Brandi Higgins BS, HT(ASCP) On Wed, May 12, 2010 at 5:57 PM, Cristi stephenson wrote: > Hello Histoland, > I was wondering if anyone out there does stains other than H&E 's on > specific tissues. For example, does anyone run stains to rule out H. > pylori on all gastric biopsies? Any information as to the standards in > other labs would be great. > Thanks, > Cristi > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet