RE: [Histonet] Gastric biopsies

2010-05-13 Thread Joyce Cline
Jennifer,

I  read my response and realize I made the mistake of stating on all esophageal 
biopsies. We only do candida
when the Endoscopy physicians list it on the paperwork. I ran out of our 
control for fungus so I am purchasing controls from American Master Tech. (they 
use superfrost plus slides which is what Ventana approves for our NexES 
stainer) The American Master Tech fungus controls stain well with Pas light 
green and GMS.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cl...@wchsys.org


From: Schumacher, Jennifer J [jschu...@fairview.org]
Sent: Thursday, May 13, 2010 10:38 AM
To: Joyce Cline
Subject: RE: [Histonet] Gastric biopsies

Joyce,
I am facinated by your routine PAS/light green on esophogeal biopsies.  We are 
talking about working this stain up in house, but have had trouble finding 
candida control tissue.  May I ask what you use or how you obtain your 
controls?  Jennifer


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline 
[joyce.cl...@wchsys.org]
Sent: Thursday, May 13, 2010 8:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Gastric biopsies

We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies 
if requested by the grossing pathologist. We do Diff-Quik on all stomach 
biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done 
on colon with a diagnosis of microscopic or collagenous colitis . A pas light 
green is done on esophogeal biopsies for candida. Any other specials are done 
at the request of the pathologist.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson 
[cls71...@sbcglobal.net]
Sent: Wednesday, May 12, 2010 5:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific 
tissues.  For example, does anyone run stains to rule out H. pylori on all 
gastric biopsies?  Any information as to the standards in other labs would be 
great.
Thanks,
Cristi
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RE: [Histonet] Gastric biopsies

2010-05-13 Thread Joyce Cline
We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies 
if requested by the grossing pathologist. We do Diff-Quik on all stomach 
biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done 
on colon with a diagnosis of microscopic or collagenous colitis . A pas light 
green is done on esophogeal biopsies for candida. Any other specials are done 
at the request of the pathologist.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson 
[cls71...@sbcglobal.net]
Sent: Wednesday, May 12, 2010 5:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific 
tissues.  For example, does anyone run stains to rule out H. pylori on all 
gastric biopsies?  Any information as to the standards in other labs would be 
great.
Thanks,
Cristi
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RE: [Histonet] Gastric biopsies

2010-05-13 Thread Weems, Joyce
For H. pylori we do a Genta, which is a modified Steiner, with Alcian blue and 
H & E over it. 

I've finally gotten the docs to wait to make sure they need specials on 
everything else, so as not to waste. J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susanfpl...@aim.com
Sent: Wednesday, May 12, 2010 21:57
To: cls71...@sbcglobal.net; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Gastric biopsies


We routinely do PAS with and without digestion, Trichrome and Iron on liver 
biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine 
Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using 
immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed 
in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC






-Original Message-
From: Cristi stephenson 
To: histonet 
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies


Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific 
issues.  For example, does anyone run stains to rule out H. pylori on all 
astric biopsies?  Any information as to the standards in other labs would be 
reat.
hanks,
risti
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Re: [Histonet] Gastric biopsies

2010-05-13 Thread Rene J Buesa
For gastric biopsies we routinely did modified Steiner for H. pylori
René J.

--- On Wed, 5/12/10, Cristi stephenson  wrote:


From: Cristi stephenson 
Subject: [Histonet] Gastric biopsies
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, May 12, 2010, 5:57 PM


Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific 
tissues.  For example, does anyone run stains to rule out H. pylori on all 
gastric biopsies?  Any information as to the standards in other labs would be 
great.
Thanks,
Cristi
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RE: [Histonet] Gastric biopsies

2010-05-13 Thread Cynthia Pyse
Christi
We run a diff stain on all gastric bx suspected of H. pylori as routine, it
saves us time. 

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi
stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on
specific tissues.  For example, does anyone run stains to rule out H.
pylori on all gastric biopsies?  Any information as to the standards in
other labs would be great.
Thanks,
Cristi
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RE: [Histonet] Gastric biopsies

2010-05-13 Thread Gill, Caula A.
Hello Cristi,

We routinely do a steiner on all Gastric Bx.'s we also do Pas W and WO 
digestion, Fe, Trichrome and retic on all liver with DX of Hepatitis. GMS-P on 
Lung bx POS. for HIV if requested by Dr. Elastic, retic and Trichrome  on 
Temporal Arteries.

Caula Gill (HT) ASCP

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi 
stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific 
tissues.  For example, does anyone run stains to rule out H. pylori on all 
gastric biopsies?  Any information as to the standards in other labs would be 
great.
Thanks,
Cristi
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Re: [Histonet] Gastric biopsies

2010-05-12 Thread susanfplaza

We routinely do PAS with and without digestion, Trichrome and Iron on liver 
biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine 
Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using 
immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed 
in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC






-Original Message-
From: Cristi stephenson 
To: histonet 
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies


Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific 
issues.  For example, does anyone run stains to rule out H. pylori on all 
astric biopsies?  Any information as to the standards in other labs would be 
reat.
hanks,
risti
__
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isto...@lists.utsouthwestern.edu
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Re: [Histonet] Gastric biopsies

2010-05-12 Thread Brandi Higgins
We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all
esophageal biopsies with Barrett's indicated by the GI Dr.  Our other
special stains are by request, but we almost always end up doing retic and
trichrome on liver bx submitted with hepatitis as Clinical Dx.  Hope this
helps.

Brandi Higgins BS, HT(ASCP)

On Wed, May 12, 2010 at 5:57 PM, Cristi stephenson
wrote:

> Hello Histoland,
> I was wondering if anyone out there does stains other than H&E 's on
> specific tissues.  For example, does anyone run stains to rule out H.
> pylori on all gastric biopsies?  Any information as to the standards in
> other labs would be great.
> Thanks,
> Cristi
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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