RE: [Histonet] IHC on FNA
Linda I agree with you that is why I asked the question. We do not have a cryostat, so frozen sections are out of the question. I thought of making cytospin slides out of positive fluid but then you run into the problem of any positive cells making it onto the slide. Would any of you run the FNA slide without pretreatment and the FFPE control with the pretreatment? I appreciate everyone suggestions. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Friday, April 09, 2010 12:40 PM To: Cynthia Pyse; Histonet Subject: RE: [Histonet] IHC on FNA This topic touches a nerve with me. We recently went back to receiving requests on FNAs after years of not doing any. When we were routinely staining them with IHC, we would use a frozen tissue section for a control as the same protocols generally worked for both, i.e. no HIER, protease, etc as may be needed for FFPE. When we stopped doing FNA IHC routinely, we eliminated our frozen section control inventory. Now that we're doing them again, it was decided to use FFPE controls and their accompanying IHC protocols. I do not agree with this practice as I feel there are too many differences between FNA preparations and FFPE sections. I was voted down so now we are using HIER, protease, etc. whatever the FFPE protocol calls for on these FNA preparations. Surprisingly, at least to me, there are still cells left on the FNA preps after even the harshest retrieval protocols. We also don't run negative controls of the FNAs. The argument being that there are totally different cells from one slide to the next so a comparison between a negative control slide and one that received antibody is useless. So that's what we're doing even though I don't agree with it. At the time this all came about, I also queried Histonet as to common practices so the archives should have some info. also. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 09, 2010 11:16 AM To: 'Histonet' Subject: [Histonet] IHC on FNA Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC on FNA
Great points, Linda! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, April 09, 2010 11:40 AM To: Cynthia Pyse; Histonet Subject: RE: [Histonet] IHC on FNA This topic touches a nerve with me. We recently went back to receiving requests on FNAs after years of not doing any. When we were routinely staining them with IHC, we would use a frozen tissue section for a control as the same protocols generally worked for both, i.e. no HIER, protease, etc as may be needed for FFPE. When we stopped doing FNA IHC routinely, we eliminated our frozen section control inventory. Now that we're doing them again, it was decided to use FFPE controls and their accompanying IHC protocols. I do not agree with this practice as I feel there are too many differences between FNA preparations and FFPE sections. I was voted down so now we are using HIER, protease, etc. whatever the FFPE protocol calls for on these FNA preparations. Surprisingly, at least to me, there are still cells left on the FNA preps after even the harshest retrieval protocols. We also don't run negative controls of the FNAs. The argument being that there are totally different cells from one slide to the next so a comparison between a negative control slide and one that received antibody is useless. So that's what we're doing even though I don't agree with it. At the time this all came about, I also queried Histonet as to common practices so the archives should have some info. also. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 09, 2010 11:16 AM To: 'Histonet' Subject: [Histonet] IHC on FNA Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC on FNA
This topic touches a nerve with me. We recently went back to receiving requests on FNAs after years of not doing any. When we were routinely staining them with IHC, we would use a frozen tissue section for a control as the same protocols generally worked for both, i.e. no HIER, protease, etc as may be needed for FFPE. When we stopped doing FNA IHC routinely, we eliminated our frozen section control inventory. Now that we're doing them again, it was decided to use FFPE controls and their accompanying IHC protocols. I do not agree with this practice as I feel there are too many differences between FNA preparations and FFPE sections. I was voted down so now we are using HIER, protease, etc. whatever the FFPE protocol calls for on these FNA preparations. Surprisingly, at least to me, there are still cells left on the FNA preps after even the harshest retrieval protocols. We also don't run negative controls of the FNAs. The argument being that there are totally different cells from one slide to the next so a comparison between a negative control slide and one that received antibody is useless. So that's what we're doing even though I don't agree with it. At the time this all came about, I also queried Histonet as to common practices so the archives should have some info. also. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 09, 2010 11:16 AM To: 'Histonet' Subject: [Histonet] IHC on FNA Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet