RE: [Histonet] Kappa/Lambda ISH on Ventana
I was informed of the ASR issue also. Back in the day, vendors, including VMS, did give out protocols for ASR probes but no longer. The best I can offer is that with the VMS EBER probe we use ISH Protease 2/12" with great results. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From: Angela Bitting [mailto:akbitt...@geisinger.edu] Sent: Friday, April 01, 2011 8:42 AM To: Steven Wilkes; histonet@lists.utsouthwestern.edu; Sebree Linda A Subject: RE: [Histonet] Kappa/Lambda ISH on Ventana Ventana can no longer give out protocols. Theses probes are ASR products and the FDA doesn't allow them to tell you the protocol. They can offer guidance if you have a protocol that doesn't work well, but they can't outright tell you a protocol. Another institution that runs Ventana probes is your best bet. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Sebree Linda A" 3/31/2011 7:09 PM >>> You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwil...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Kappa/Lambda ISH on Ventana
Ventana can no longer give out protocols. Theses probes are ASR products and the FDA doesn't allow them to tell you the protocol. They can offer guidance if you have a protocol that doesn't work well, but they can't outright tell you a protocol. Another institution that runs Ventana probes is your best bet. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Sebree Linda A" 3/31/2011 7:09 PM >>> You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwil...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Kappa/Lambda ISH on Ventana
You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwil...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
