RE: [Histonet] Microtome knives
I might also add that Delaware Diamond Knives (DDK) also sells and sharpens microtome knives! Jack Jack L RatliffRatliff Histology Consultants, LLC317-281-1975 > From: pru...@ihctech.net > To: max_histo...@yahoo.it; jkr...@deltacollege.edu; > Histonet@lists.utsouthwestern.edu > Date: Sun, 18 Nov 2012 07:50:11 -0700 > Subject: RE: [Histonet] Microtome knives > CC: > > There are knife sharpening services Sturkey and Dorn and Hart are two that > come to mind. You can also find refurbished disposable blade holders and > buy disposable blades, the blade holder you get will determine if you use > low profile or high profile blades. > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting, LLC > 40864 Arkansas Ave > Bennett, CO 80102 > Phone: 303-644-4538 > Fax: 720-859-4110 > pru...@ihctech.net > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo > Sent: Sunday, November 11, 2012 2:45 AM > To: Jon Krupp; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Microtome knives > > I prefer to sharpen my microtome knives by myself by hand. > I have a vintage Cambridge Rocking Microtome and despite its age it works > very well. > Sharpening is a time consuming for the first time, it's depends on the > conditions of the blade edge. > Once you have a nice cutting profile its maintenance it's quite easy and it > takes a few minutes by > stroking the knife on a flat glass with oil and a bit of aluminium oxide > powder (3 -1 micron grits). > For me sharpening and honing of a microtome knife has became a secondary > "hobby". > A solid knife has the advantage, compared to a disposable blade, to be > liable to less vibrations. > > Kind Regards, > Massimo Tosi > > > "A humble Chemical > Engineer who loves Histology" > > > > > > > Da: Jon Krupp > A: Histonet@lists.utsouthwestern.edu > Inviato: Venerdì 9 Novembre 2012 19:49 > Oggetto: [Histonet] Microtome knives > > Greetings > > I need some advice regarding microtome knives. I am not histo tech, I did > all my sectioning in a plant research lab, but now I find myself needing to > learn more about histo type methods. > > We have microtomes, AO 820's, and we have a bunch of donated knives. I need > advice about whether it would be better to find a knife sharpener and use > the microtome knives we have, or check into getting a disposable knife > holder. > > When I was sectioning, we just used a simple razor blade holder. Now I see > references to high profile and low profile blades and holders, and I don't > know the difference. > > Anyone willing to help me out? > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkr...@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome knives
There are knife sharpening services Sturkey and Dorn and Hart are two that come to mind. You can also find refurbished disposable blade holders and buy disposable blades, the blade holder you get will determine if you use low profile or high profile blades. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pru...@ihctech.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo Sent: Sunday, November 11, 2012 2:45 AM To: Jon Krupp; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome knives I prefer to sharpen my microtome knives by myself by hand. I have a vintage Cambridge Rocking Microtome and despite its age it works very well. Sharpening is a time consuming for the first time, it's depends on the conditions of the blade edge. Once you have a nice cutting profile its maintenance it's quite easy and it takes a few minutes by stroking the knife on a flat glass with oil and a bit of aluminium oxide powder (3 -1 micron grits). For me sharpening and honing of a microtome knife has became a secondary "hobby". A solid knife has the advantage, compared to a disposable blade, to be liable to less vibrations. Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" Da: Jon Krupp A: Histonet@lists.utsouthwestern.edu Inviato: Venerdì 9 Novembre 2012 19:49 Oggetto: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkr...@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome knives
Hi All, I am Sharon Harrison, supervisor of Histopathology lab at UWI Mona in Kingston Jamaica. I agree with you that we still need to have the solid blades for the cutting of hard tissue such as cervix fibroids and bone. Hence we have to maintain some vestige of the old technology of knife sharpening, as the disposable blades are quite expensive. The disposable blades are great for biopsies and softer tissue but a real pain for getting sections of the harder tissues. Therefore I advice having both systems especially if your laboratory handles all types of tissue specimen. Sharon Harrison Chief Medical Technologist DMT, BSc, MPH From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo [max_histo...@yahoo.it] Sent: Sunday, November 11, 2012 4:44 AM To: Jon Krupp; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome knives I prefer to sharpen my microtome knives by myself by hand. I have a vintage Cambridge Rocking Microtome and despite its age it works very well. Sharpening is a time consuming for the first time, it's depends on the conditions of the blade edge. Once you have a nice cutting profile its maintenance it's quite easy and it takes a few minutes by stroking the knife on a flat glass with oil and a bit of aluminium oxide powder (3 -1 micron grits). For me sharpening and honing of a microtome knife has became a secondary "hobby". A solid knife has the advantage, compared to a disposable blade, to be liable to less vibrations. Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" Da: Jon Krupp A: Histonet@lists.utsouthwestern.edu Inviato: Venerdì 9 Novembre 2012 19:49 Oggetto: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkr...@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome knives
I prefer to sharpen my microtome knives by myself by hand. I have a vintage Cambridge Rocking Microtome and despite its age it works very well. Sharpening is a time consuming for the first time, it's depends on the conditions of the blade edge. Once you have a nice cutting profile its maintenance it's quite easy and it takes a few minutes by stroking the knife on a flat glass with oil and a bit of aluminium oxide powder (3 -1 micron grits). For me sharpening and honing of a microtome knife has became a secondary "hobby". A solid knife has the advantage, compared to a disposable blade, to be liable to less vibrations. Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" Da: Jon Krupp A: Histonet@lists.utsouthwestern.edu Inviato: Venerdì 9 Novembre 2012 19:49 Oggetto: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkr...@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome knives
Hi Jon, Depends on your cash flow. You could get a used sharpener somewhere off the web somewhere such as http://www.labx.com/v2/adsearch/resultsnew.cfm?sw=sharpener&mr=25&te=cat , or http://www.medwow.com/used-microtome-knife-sharpener-equipment/63.med but sharpening knives is a pain IMO and steel knives present more of a safety hazard. I would recommend a sharpener that uses the glass honing plates. You would also need the coarse and fine abrasives. Personally, I would opt for a low profile disposable blade holder that fits your 820. Low and high profile refer to the size (height) of the blade. We use low profile for paraffin block sectioning and high profile for cryostat sectioning. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jon Krupp Sent: Friday, November 09, 2012 1:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkr...@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome knives
Because of your donated knives you will have to buy a knives sharpener which are costly and not very easy to find. Your best option is to buy a high profile disposable blades holder (that will be cheaper), buy disposable blades and avoid all the frustrations and waste of time sharpening knives. René J. From: Jon Krupp To: Histonet@lists.utsouthwestern.edu Sent: Friday, November 9, 2012 1:49 PM Subject: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkr...@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
