Hi Chris, CAP actually has a specific checklist question on Negative Controls. The question is ANP.22570 and if I remember correctly it is quite extensive. Most of the CAP questions are about 7 to 10 lines. The Negative control question is more than a page long. Get your hands a copy of the most current checklist for anatomic pathology - That will include Immunohistochemistry.
By the way, the rule is that a negative control must be included with each run and there is much, much more. ANP.22570 Good Luck, Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Friday, July 13, 2012 9:31 PM To: histonet@lists.utsouthwestern.edu Cc: cjac...@clinpath.com Subject: [Histonet] Negative controls and CAP Histonetters, I have been made the IHC lead in a fairly large laboratory that is seeking to become CAP accredited. I am definitely a newbie when it comes to CAP. A question was brought up today about how we do our negative controls. Specifically, should we run another negative control with any IHCs that need to be repeated or if we should run another negative control if at a later date a pathologist orders additional IHCs on a case. With our current protocol, we run one negative control per detection kit used, per case. Consequently, most cases end up with just one negative control slide. We do NOT run another negative control if we have to repeat one of a group of immunos or if a pathologist orders additional stains at a later point. I am wondering if this is good practice, and acceptable with CAP. I would love to find some literature that could help me make a case either way on this. Actually, any literature or pointers regarding IHCs and becoming CAP accredited will probably help save what little hair I have left. Thank you, Chris Jacobs, HT(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet