RE: [Histonet] RE: Cutting, Processing, etc
Whilst value for money and costings rule then the "management" will always look to save $$$s by hiring semi/unskilled personnel.the other point in this thread is that people are increasingly using the Histonet as an on-line and up to date text, which is surely not a bad thing?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: 17 September 2010 22:09 To: james leroux Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Hi James, I would take it a step farther with the continuing ed. I think it's beyond the supervisors it gets up into lab administration (clinical lab world). I personally know of a group of great folks that work hard and run a quality service. In the last 3 years they've had a major drop off in their continuing ed. And it, of course, is tied to the budget. Unfortunately, in this case (my view) those making the money decisions are missing the value. It seems they're unwilling to make the investment. I fear that in 5 years or less (if it continues) this service will suffer. I suspect there are other folks out there in the same boat. My hat is off to everyone out there working hard in our field and to the "enlightened" administrators and physicians that advocate continuing ed. Have a great weekend. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of james leroux Sent: Friday, September 17, 2010 10:55 AM To: 'Nails, Felton'; histot...@imagesbyhopper.com; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some "supervisors" around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 11:03 AM To: 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answ
RE: [Histonet] RE: Cutting, Processing, etc
Hi James, I would take it a step farther with the continuing ed. I think it's beyond the supervisors it gets up into lab administration (clinical lab world). I personally know of a group of great folks that work hard and run a quality service. In the last 3 years they've had a major drop off in their continuing ed. And it, of course, is tied to the budget. Unfortunately, in this case (my view) those making the money decisions are missing the value. It seems they're unwilling to make the investment. I fear that in 5 years or less (if it continues) this service will suffer. I suspect there are other folks out there in the same boat. My hat is off to everyone out there working hard in our field and to the "enlightened" administrators and physicians that advocate continuing ed. Have a great weekend. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of james leroux Sent: Friday, September 17, 2010 10:55 AM To: 'Nails, Felton'; histot...@imagesbyhopper.com; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some "supervisors" around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 11:03 AM To: 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my water
RE: [Histonet] RE: Cutting, Processing, etc
I echo Joyce's point. W/o all info, I hesitate to jump to conclusions. I was had an efficiency expert following me around for a week at the insistance of some administrators (it wasn't just me, but the entire lab)These are the types of silly questions these "experts" might ask, wondering if they can save 47 seconds out of the day. If anything is happening to our field, it might be the tampering by non-technicians in our technical duties in the name of "stream-lining" or keeping their tush's covered for their boss who is keeping her tush covered from ad nausium. It's a cynical Fridaybut the good news is that my Kindle is being delivered today! - Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 17, 2010 12:07 PM To: Nails, Felton; 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My 2 cents is that she needed to convince someone this was how it is done! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 13:03 To: 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely
RE: [Histonet] RE: Cutting, Processing, etc
They do say that in the next 10 years 70% or so of us will be ret
ired. I am 30, and a rarity it seems. I think the main issue wi= th
people not picking up histology is because it is a fairly unknown
field.=I also think that with ASCP slowly moving towards having to
have a b= achelors degree the field is not updating itself. A college
graduate = starting in the world making the same pay they could
working at Walmart doe= sn't lend itself to that person learning or
staying in histology. I k= now the more experience you have the more
you make, but it still isn't enou= gh. I have been in the histo.
world since '98 and the pay scales real= ly haven't changed all that
much. I think the main solution would be = if you want college
educated, skilled HTs out there...pay them like a skill= ed college
graduate!! 35K a year for some places to start? How = do you pay
$400 a month in student loans and live with that salary? J= ust my 2
cents...
Oh and Amy...there are no stupid= questions!!! You go girl, learn
histology it's a great field. = Fair warning though...you will start
talking to yourself...it's a histo. th= ing =)
Sarah Goebel, B.A., HT (ASCP)
Histotechnician
XBiotech USA Inc.
8201 East Riverside Dr. Bldg= 4 Suite 100
Austin, Texas 78744
<= em>
(512)386-5= 107
Original Message ----
Subject: RE: [Histonet] RE: Cutting, Processing, etc
From: "Nails, Felton" <[1]fln= a...@texaschildrens.org>
Date: Fri, September 17, 2010 10:03 am
To: "'[2]histot...@imagesbyhop= per.com'" <[3]histotech
@imagesbyhopper.com>,
"'mohamed abd el razik'" <[4]k8...@yahoo= .com>,
"[5]histo...@lists.utsout= hwestern.edu" <[6]
Histonet@lists.utsouthwestern.edu>
As I look through and monitor questions, it is apparent that our field
is d= eclining. These are very basic questions not about special
stains or IHC st= ains but basic histology that should have been
taught in histology 101. My = fear is that as we get older and leave
the field, who and what will be left= to carry the torch. Those of you
who ask, don't take offense to my thought= s but take action and pick
up a book and read. You will improve yourself an= d the field.
Just my thoughts, if I offended you it was not my intent.
-Original Message-
From: [7]histonet= -boun...@lists.utsouthwestern.edu
[[8]mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
[9]histot...@= imagesbyhopper.com
Sent: Friday, September 17, 2010 11:42 AM
To: 'mohamed abd el razik'; [10]histo...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Cutting, Processing, etc
My first reaction to the "what is happening to our field", was WOW. It
see= med unkind to me, as if they original poster should not have
asked these qu= estions. With further reading of the replies to this
post, I am not so sur= e it was an unkind response, but one of
potential shock and dismay to the i= dea that labs might not be
producing the quality work that most of us empl= oy on a daily basis.
Amy, in answer to your questions, I will echo some of the sentiments
that I= have read here.
1. Facing of blocks. We use one blade to face blocks and another, new
blad= e when we do our actual sectioning. In my case, I face as many
as I can, k= nowing I am going to toss that knife when I am done
facing.
2. Soaking of blocks. After facing my blocks, I will put them on a
cold, = damp, ice cube tray. This will achieve two purposes for me, a)
to chill th= e block and b) to introduce moisture into the faced
tissue. If I get a blo= ck that is particularly dry or hard (some
calcified tissues for example), I= will face them, put them face down
on my waterbath and allow the hot water= to penetrate into the tissue
for 15-45 seconds, depending on the block.
After cooling on the ice tray, they usually "cut like butter" for me.
Typically, my blocks are not on the ice cubes for more than 15
minutes. As= I cut some, I will rotate the blocks around the ice tray,
adding more "war= m"
blocks for cooling.
3. Freeze spray. I hardly ever use the freeze spray. About the only
time= I find that I need it is if I have a particularly fatty tissue
and it does= n't want to section.
4. Tissue processor changes. This is definitely something that is
"site s= pecific". In our case, we do base it on volumes. If we have a
small volume= of our "little" biopsies, we might not change the
machine we
RE: [Histonet] RE: Cutting, Processing, etc
Felton, I would have to disagree with your assessment of the emails. Our field is very strong and is not in decline. Unfortunately, some "supervisors" around the country are relying on archaic methods and do not want to see or welcome change within the histo lab. We call ourselves professionals and yet not all of us are required to do continuing education? I read emails everyday and laugh at some of the bloviating that goes on inside this forum. I am glad the questions are asked, but I am also amazed at some of the responses that are shared with everyone. I choose to respond one on one with the person asking the question. Basic histology deals with didactics and this particular inquiry dealt more with OJT. There are many ways to get the same job done; are there more efficient ways? Probably, but this does not mean we all do our job the same way. I am not concerned about the future of Histotechnology. I embrace the opportunity to teach the young technicians about a field that sees a change almost daily. I am not here to offend either, but rather to defend an occupation that is as fascinating as it is frustrating. Respectfully, James Leroux, AAS, BA, HTL Histology Supervisor Petroglyph Pathology 640 Quantum Rd. Rio Rancho, NM 87124 (505) 924-0219 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 11:03 AM To: 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot
RE: [Histonet] RE: Cutting, Processing, etc
My 2 cents is that she needed to convince someone this was how it is done! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, September 17, 2010 13:03 To: 'histot...@imagesbyhopper.com'; 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many o
RE: [Histonet] RE: Cutting, Processing, etc
As I look through and monitor questions, it is apparent that our field is declining. These are very basic questions not about special stains or IHC stains but basic histology that should have been taught in histology 101. My fear is that as we get older and leave the field, who and what will be left to carry the torch. Those of you who ask, don't take offense to my thoughts but take action and pick up a book and read. You will improve yourself and the field. Just my thoughts, if I offended you it was not my intent. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, September 17, 2010 11:42 AM To: 'mohamed abd el razik'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Cutting, Processing, etc My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the
RE: [Histonet] RE: Cutting, Processing, etc
My first reaction to the "what is happening to our field", was WOW. It seemed unkind to me, as if they original poster should not have asked these questions. With further reading of the replies to this post, I am not so sure it was an unkind response, but one of potential shock and dismay to the idea that labs might not be producing the quality work that most of us employ on a daily basis. Amy, in answer to your questions, I will echo some of the sentiments that I have read here. 1. Facing of blocks. We use one blade to face blocks and another, new blade when we do our actual sectioning. In my case, I face as many as I can, knowing I am going to toss that knife when I am done facing. 2. Soaking of blocks. After facing my blocks, I will put them on a cold, damp, ice cube tray. This will achieve two purposes for me, a) to chill the block and b) to introduce moisture into the faced tissue. If I get a block that is particularly dry or hard (some calcified tissues for example), I will face them, put them face down on my waterbath and allow the hot water to penetrate into the tissue for 15-45 seconds, depending on the block. After cooling on the ice tray, they usually "cut like butter" for me. Typically, my blocks are not on the ice cubes for more than 15 minutes. As I cut some, I will rotate the blocks around the ice tray, adding more "warm" blocks for cooling. 3. Freeze spray. I hardly ever use the freeze spray. About the only time I find that I need it is if I have a particularly fatty tissue and it doesn't want to section. 4. Tissue processor changes. This is definitely something that is "site specific". In our case, we do base it on volumes. If we have a small volume of our "little" biopsies, we might not change the machine weekly, but every two weeks. Generally our large specimen machine is changed weekly. Your mileage may vary! :o) Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, September 17, 2010 5:38 AM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Cutting, Processing, etc i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized r
Re: [Histonet] RE: Cutting, Processing, etc
i think that histonet is a primary educational group for all levels and any expert in our feild have asked these quistions one day befor and we should ask freely without any shame . i'm as begainner have learned alot from these little quistions. and i asked befor for name of antibodies and its use to be written to clear the information for all levels and no shame for that. --- On Thu, 9/16/10, Nails, Felton wrote: From: Nails, Felton Subject: [Histonet] RE: Cutting, Processing, etc To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, September 16, 2010, 6:06 PM what is happening to our field?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! I have some questions about procedures in different histo labs and I'd like to have some 'backup' when people look at me like I'm crazy here... After embedding, you face (trim) your blocks, right? Do you take sections right from that same blade, or move/change your blade? How many of you 'soak' your blocks in water/softblock before cutting them? Do you put them on a cold plate/use ice and water, etc? Does this depend on the type of tissue, or do you treat them all the same? How often is "freezy" spray used in your lab, and where and when do you use it? How often do you rotate/change your reagents in your processors? Do you calculate this by how many blocks/days/weeks of use? Thank you so much for your input!! Amy Senn, HT Holy Spirit Hospital, Camp Hill PA Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Cutting, Processing, etc
DON'T get me started... And I have only been practicing 9 years! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Nails, Felton Sent: Thu 9/16/2010 11:06 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cutting, Processing, etc what is happening to our field?? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Thursday, September 16, 2010 10:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting, Processing, etc Hello Histoland! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
