RE: [Histonet] RE: Storage of HER2 neu control slides
Just started using it ( currently doing validation & correlation), so maybe will be issues at some later time. I just noticed the "overnight" in the IFU file from their website when I was putting the SOP together. I have to be honest, that I did my standard preparation so far and it worked great, and had no issues with fall off or staining. Since it is IVD, was considering in my mind, if shorter baking would be a "modification"? The kit I have uses CB11. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lseb...@uwhealth.org To: joellewea...@hotmail.com CC: histonet@lists.utsouthwestern.edu; richard.car...@hhchealth.org Subject: RE: [Histonet] RE: Storage of HER2 neu control slides Date: Mon, 14 Jul 2014 14:59:41 + Thanks Joelle. I’m surprised that Oracle is suggesting overnight baking as I noticed a huge difference when I dried some test tissue controls for 30” @ 60 degrees vs 10” @ 60 degrees. I was testing some blocks that I had 3 cores of varying degrees of intensity. All showed much decreased reactivity at 30” vs 10”. In fact, the 2 weaker cores were virtually negative. I can only assume you’re using a different clone than we are and that there is different epitope stability between clones. Thanks for your response, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From: Joelle Weaver [mailto:joellewea...@hotmail.com] Sent: Monday, July 14, 2014 9:25 AM To: Cartun, Richard; Sebree Linda A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Storage of HER2 neu control slides I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: richard.car...@hhchealth.org > To: lseb...@uwhealth.org; Histonet@lists.utsouthwestern.edu > Date: Mon, 14 Jul 2014 14:22:43 + > CC: > Subject: [Histonet] RE: Storage of HER2 neu control slides > > We keep all of our positive control slides at RT. The breast predictive > marker slides often sit for a few weeks (after cutting) before being used. I > think everyone's situation will be different due to laboratory humidity, > tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.car...@hhchealth.org > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A > Sent: Monday, July 14, 2014 8:44 AM > To: Histonet (Histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Storage of HER2 neu control slides > > Good morning, > > I'd like to get some opinions on the storage of positive control slides for > HER2 neu. We currently keep a number of positive control slides in a -20 > degree freezer as some antigens tend to lose reactivity at room temperature; > ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? > > Thanks for any and all replies, > > Linda > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Storage of HER2 neu control slides
Thanks Joelle. I'm surprised that Oracle is suggesting overnight baking as I noticed a huge difference when I dried some test tissue controls for 30" @ 60 degrees vs 10" @ 60 degrees. I was testing some blocks that I had 3 cores of varying degrees of intensity. All showed much decreased reactivity at 30" vs 10". In fact, the 2 weaker cores were virtually negative. I can only assume you're using a different clone than we are and that there is different epitope stability between clones. Thanks for your response, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From: Joelle Weaver [mailto:joellewea...@hotmail.com] Sent: Monday, July 14, 2014 9:25 AM To: Cartun, Richard; Sebree Linda A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Storage of HER2 neu control slides I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: richard.car...@hhchealth.org<mailto:richard.car...@hhchealth.org> > To: lseb...@uwhealth.org<mailto:lseb...@uwhealth.org>; > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > Date: Mon, 14 Jul 2014 14:22:43 + > CC: > Subject: [Histonet] RE: Storage of HER2 neu control slides > > We keep all of our positive control slides at RT. The breast predictive > marker slides often sit for a few weeks (after cutting) before being used. I > think everyone's situation will be different due to laboratory humidity, > tissue fixation, and tissue processing which removes the water from the > tissue which has been shown to be the one of the culprits in antigen > degradation in stored unstained slides. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.car...@hhchealth.org<mailto:richard.car...@hhchealth.org> > > > -Original Message- > From: > histonet-boun...@lists.utsouthwestern.edu<mailto:histonet-boun...@lists.utsouthwestern.edu> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda > A > Sent: Monday, July 14, 2014 8:44 AM > To: Histonet > (Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>) > Subject: [Histonet] Storage of HER2 neu control slides > > Good morning, > > I'd like to get some opinions on the storage of positive control slides for > HER2 neu. We currently keep a number of positive control slides in a -20 > degree freezer as some antigens tend to lose reactivity at room temperature; > ER and PR come to mind. What has peoples' experiences been with the storage > of these control slides? > > Thanks for any and all replies, > > Linda > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Storage of HER2 neu control slides
I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: richard.car...@hhchealth.org > To: lseb...@uwhealth.org; Histonet@lists.utsouthwestern.edu > Date: Mon, 14 Jul 2014 14:22:43 + > CC: > Subject: [Histonet] RE: Storage of HER2 neu control slides > > We keep all of our positive control slides at RT. The breast predictive > marker slides often sit for a few weeks (after cutting) before being used. I > think everyone's situation will be different due to laboratory humidity, > tissue fixation, and tissue processing which removes the water from the > tissue which has been shown to be the one of the culprits in antigen > degradation in stored unstained slides. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.car...@hhchealth.org > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A > Sent: Monday, July 14, 2014 8:44 AM > To: Histonet (Histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Storage of HER2 neu control slides > > Good morning, > > I'd like to get some opinions on the storage of positive control slides for > HER2 neu. We currently keep a number of positive control slides in a -20 > degree freezer as some antigens tend to lose reactivity at room temperature; > ER and PR come to mind. What has peoples' experiences been with the storage > of these control slides? > > Thanks for any and all replies, > > Linda > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
