We use acetone to denature fatty tumors without any problems. They sit in it for at least half an hour. I don't rinse them.
Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Saturday, November 15, 2014 2:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Acetone fixation Sheree H (where?) asks: >>Have questions about using acetone at the grossing counter. Does >>anyone have problems with it being carried over into the formalin on the processor? We rinse, open cassette to let it dry completely and even change the cassette. I've had issues with my processor and want to make sure it's not coming from the acetone.<< Acetone doesn't belong in the grossing area - too much of a fire and explosion hazard. If your pathologists are using it to fix marking ink onto specimens, they can use 3% acetic acid (half-strength white vinegar). I don't use anything at all - if you blot the specimen dry before you put the ink on, it'll stay put. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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