RE: [Histonet] Schiffs

[Grantham, Andrea L - (algranth)]

I bought Schiff's from Newcomer and it could be stored at RT. 

Andi G.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Joelle Weaver 
[joellewea...@hotmail.com]
Sent: Saturday, April 18, 2015 3:45 AM
To: John Kiernan; Bernice Frederick; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Schiffs

The reagent we use for our manual backup stains using Schiffs states 
refrigeration for long term storage only.


Joelle Weaver MAOM, HTL (ASCP) QIHC





> From: jkier...@uwo.ca
> To: b-freder...@northwestern.edu; histonet@lists.utsouthwestern.edu
> Date: Sat, 18 Apr 2015 01:26:34 -0500
> Subject: Re: [Histonet] Schiffs
> CC:
>
> Schiff's reagent does not need to be refrigerated. It just needs to be 
> screw-capped so that it doesn't decompose by loss of sulphur dioxide. For 
> decades, Schiff has been stored in many labs at 4C for no good reason. It is 
> used at room temperature in both of its histochemical applications: the 
> Feulgen and PAS reactions.
>
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = =
> On 17/04/15, Bernice Frederick   wrote:
> > Am I missing something? I ordered Schiffs and sigma tells me it was shipped 
> > yesterday. Hello, today is Friday and last I recall Schiffs need to be 
> > refrigerated! You'd..
> > think they would realize this. Sorry all, have to vent.
> > Bernice
> >
> > Bernice Frederick HTL (ASCP)
> > Senior Research Tech
> > Pathology Core Facility
> > Robert. H. Lurie Cancer Center
> > Northwestern University
> > 710 N Fairbanks Court
> > Olson 8-421
> > Chicago,IL 60611
> > 312-503-3723
> > b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu 
> > >
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
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RE: [Histonet] Schiffs

[Joelle Weaver]

The reagent we use for our manual backup stains using Schiffs states 
refrigeration for long term storage only. 


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
> From: jkier...@uwo.ca
> To: b-freder...@northwestern.edu; histonet@lists.utsouthwestern.edu
> Date: Sat, 18 Apr 2015 01:26:34 -0500
> Subject: Re: [Histonet] Schiffs
> CC: 
> 
> Schiff's reagent does not need to be refrigerated. It just needs to be 
> screw-capped so that it doesn't decompose by loss of sulphur dioxide. For 
> decades, Schiff has been stored in many labs at 4C for no good reason. It is 
> used at room temperature in both of its histochemical applications: the 
> Feulgen and PAS reactions. 
> 
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = =
> On 17/04/15, Bernice Frederick   wrote:
> > Am I missing something? I ordered Schiffs and sigma tells me it was shipped 
> > yesterday. Hello, today is Friday and last I recall Schiffs need to be 
> > refrigerated! You'd..
> > think they would realize this. Sorry all, have to vent.
> > Bernice
> > 
> > Bernice Frederick HTL (ASCP)
> > Senior Research Tech
> > Pathology Core Facility
> > Robert. H. Lurie Cancer Center
> > Northwestern University
> > 710 N Fairbanks Court
> > Olson 8-421
> > Chicago,IL 60611
> > 312-503-3723
> > b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu 
> > >
> > 
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > 
> > 
> ___
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> Histonet@lists.utsouthwestern.edu
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Re: [Histonet] Schiffs

[John Kiernan]

Schiff's reagent does not need to be refrigerated. It just needs to be 
screw-capped so that it doesn't decompose by loss of sulphur dioxide. For 
decades, Schiff has been stored in many labs at 4C for no good reason. It is 
used at room temperature in both of its histochemical applications: the Feulgen 
and PAS reactions. 

John Kiernan
Anatomy, UWO
London, Canada
= = =
On 17/04/15, Bernice Frederick   wrote:
> Am I missing something? I ordered Schiffs and sigma tells me it was shipped 
> yesterday. Hello, today is Friday and last I recall Schiffs need to be 
> refrigerated! You'd..
> think they would realize this. Sorry all, have to vent.
> Bernice
> 
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-freder...@northwestern.edu >
> 
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> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
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Re: [Histonet] Schiffs

[Jennifer MacDonald]

Schiff into 10 mL of 37-40% formaldehyde



From:   Bernice Frederick 
To: "Histonet@lists.utsouthwestern.edu" 

Date:   04/13/2015 12:40 PM
Subject:[Histonet] Schiffs
Sent by:histonet-boun...@lists.utsouthwestern.edu



Having a brain fart all- does the Schiff go into the formaldehyde or vice 
versa to test if the Schiffs is still good?
Bernice


Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

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