Re: [Histonet] silver nitrate
Tasha, 1. Try 10% Potassium Iodide (KJ) for 5-10 minutes, spot will turn to yellow as lemon, then apply 10% Sodum Thiosulfate, wipe it and then talc powder and dry. 2. 15% sodium chloride with some drops hydrochloric acid, then apply 10% Sodum Thiosulfate, wash and dry. Maxim Peshkov, Russia, Taganrog. You wrote: So does anyone out there have a secret formula to get silver stain off counter tops? Thanks for you the help!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) mailto:maxim...@mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] silver nitrate
Ill give it a try! Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From: Sue [mailto:suetp...@comcast.net] Sent: Tuesday, July 01, 2014 11:23 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] silver nitrate You can rub salt on it and it should come up, I our salt on counter and make a thick scrub You can use Iodine and than wash with Sodum Thiosulfate Both work on hands the second one is a little more difficult to work with but does work ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] silver nitrate
You can rub salt on it and it should come up, I our salt on counter and make a thick scrub You can use Iodine and than wash with Sodum Thiosulfate Both work on hands the second one is a little more difficult to work with but does work ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silver in Nucleolus
Hi Jerry, Are you sure they are not AgNOR's: Lindner, L. E. (1993). Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). Journal of Histochemistry & Cytochemistry, 41(3), 439-445. Thiebaut, F., Rigaut, J. P., & Reith, A. (1984). Improvement in the Specificity of the Silver Staining Technique for Ag NOR-ASSOCIATED Acidic Proteins in Paraffin Sections. Biotechnic & Histochemistry, 59(3), 181-185. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jerry sedgewick Sent: Friday, 24 January 2014 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver in Nucleolus I'm working with a lab that stained cat gonads with Warthin Starry. We're looking for Bartonella bacteria. In the first batch that was stained, some nucleii filled with the silver stain, something I've see in published images. In the second batch it appears that nucleolus filled with silver. I haven't seen that in google searches, etc. I wondered if others have seen the nucleolus fill with silver (or is this bartonella?). I'm also wondering how to prevent this sort of thing with proper bench procedures. Thanks! Jerry Sedgewick ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] silver stain on slide-mounted FFPE brain tissues
Dr. Dean, Histotechs are familiar with brain degeneration "Silver Stains" on FFPE cut sections(slides). Common silver stains include Bielchowsky's method for neuritic plaques and neurofibrillary tangles (most requested by my pathologists for degenerative brain disorders), Bodian's method for nerve endings and fibers , Holmes method for axons and nerve fibers, or Palmgren's silver impregnation method for neural fibers. There are other histochemical stains, but these are most common on silvers on brain sections. We have been making our own solutions for years (and years and years...). There are some kits for staining available through vendors such as Newcomer Supply http://www.newcomersupply.com/products-standard-special-stains.php, or American Master Tech http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=KTBIE, and if you know the individual reagents you need, you can buy them from almost all vendors. I have not used (nor have an affiliation with) Newcomer Supply and American Master Tech brain kits, but their other products have been great. As for staining whole chunks, I have not seen or done this. It sounds interesting. Hope this helps, Hugh, HTL (ASCP) Pathology core manager Hawaii > From: robin_d...@compbio.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 25 Apr 2011 15:09:49 -0700 > Subject: [Histonet] silver stain on slide-mounted FFPE brain tissues > > Hi Histonetters, > > > > We want to do a degeneration-selective silver stain on slide-mounted > formalin-fixed paraffin-embedded monkey brain sections. It looks like most > of the kits/protocols are for free floating tissue sections or chunks of > tissue that are stained and then sectioned. Has anyone used this stain or a > similar stain on slide -mounted brain sections? (something more like an > IHC protocol). We need to do multiple different stains on sections from the > same brain, so we don't want to silver stain a whole chunk of brain tissue, > just some individual sections. I'd appreciate any suggestions or help anyone > might have to offer. > > > > Thank you, > > > > Robin > > > > Robin R. Dean, Ph.D. > > Senior Scientist & Study Director > > Comparative Biosciences, Inc. > > 786 Lucerne Dr. > > Sunnyvale, CA > > (408) 738-8060 > > robin_d...@compbio.com > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silver
I thought Histotechs were supposed to have purple thumbs. :) (You know, gardeners have green thumbs...) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of O'Donnell, Bill Sent: Tue 9/7/2010 4:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver I know... I should wear gloves when doing a GMS.I... know... that. (sorry, I thought this was Facebook for a second) Have a great week! - Sir Bill of the Blackened Thumb ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Silver Lips and Fingers
Beauty mark - like Cindy Crawford has. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Mar 23, 2010, at 9:14 AM, Breeden, Sara wrote: I'm not a chemist and may shoot myself in the foot here, but if gold chloride tones silver, would it work on skin? Then you could call it a beauty spot? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silver Nitrate instead of inking??
Dear Sheila, I would strongly recommend that you obtain approval from the manufacturer of the tissue processors into which you will be putting these tissues before you would begin this practice. The negative accumulative affect on several of the internal components of the instrument can (and probably will) eventually cause the instrument to fail. You would see the effect of the silver nitrate on any metal products it would come into contact with: forceps, metal holding container for the racks or baskets, the racks or baskets themselves if they are metal. It is not approved for use on any model of Tissue Tek Tissue Processors. Many years ago this practice was tolerated by the old tissue-transfer processors. Some people even used this inking method and placed tissues on earlier models of the fluid-transfer processors. Service that was required in those years did not include investigation as to what was placed into the instrument (not simply the reagents, but what also came in on the specimens) that could have been the root cause of the problem. I hope this informative and that you continue to use your current inking product or investigate others that are safer for you and your processors. With kind regards and wonderful holiday wishes, Nancy Klemme EduSvcsDir - Sakura Finetek -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Thursday, December 17, 2009 3:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver Nitrate instead of inking?? Hi Everyone, Has anyone ever heard of using Silver Nitrate for inking skin bx's before processing??? One of our paths mentioned it. Thanks in advance. Sheila Adey HT MLT _ Eligible CDN College & University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Silver Nitrate instead of inking??
Silver nitrate will react in the skin and transform in silver chloride BUT in order to get black it needs to be exposed to solar or very strong light, and the reaction is not instantaneous. Your PT seems that at some point in his/her life s/he got silver nitrate in the skin that transformed into a black stain. but that will not readily "fly" in a lab setting. René J. From: sheila adey To: histonet@lists.utsouthwestern.edu Sent: Thu, December 17, 2009 6:15:17 AM Subject: [Histonet] Silver Nitrate instead of inking?? Hi Everyone, Has anyone ever heard of using Silver Nitrate for inking skin bx's before processing??? One of our paths mentioned it. Thanks in advance. Sheila Adey HT MLT _ Eligible CDN College & University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Silver Nitrate
Dear Andi, For staining I'm sure it will make no difference which type of silver nitrate you use. This compound is available at 99.% purity for analytical purposes! My experience of silver staining has been mostly with nervous tissue, for which the ACS reagent grade (99%) is fine. If a silver stain goes wrong, it's very unlikely to be the fault of the AgNO3. Solutions of silver nitrate (in distilled or deionized water) can be kept for years in brown glass bottles, and can be re-used many times. filter out any bits of sections before returning the solution to its bottle. If a solution of silver nitrate has a greyish tinge it should be replaced; such a solution is likely to deposit and amplify "dirt". Be careful not to contaminate silver nitrate with anything containing chloride (that includes tap water), phosphate or carbonate ions. These all form insoluble light-sensitive silver salts and will spoil a solution of silver nitrate. Silver nitrate is ridiculously expensive and needs to be looked after. John A. Kiernan Department of Anatomy & Cell Biology The University of Western Ontario London, Canada = = = - Original Message - From: Andrea Grantham Date: Wednesday, July 8, 2009 12:00 Subject: [Histonet] Silver Nitrate To: HISTONET > After a recent run on silver stains here I need to order > Silver > Nitrate. Can anyone tell me what the difference is (except the > cost) > between Silver Nitrate > Certified ACS, USP, p.a., Extra Pure, and Ultra Pure? They all > have > the same chemical formula and FW of 169.87. My last batch of > Silver > Nitrate was probably ordered and received here just after > the > Mayflower landed and there is no information on the bottle any > longer. > Which one do I need for stains like GMS, Sevier Munger, etc.? > > Thanx, > > Andi > 8-) > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cell Biology and Anatomy > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algra...@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > "happy slicing and dicing and may all your stains work > perfectly" - > Paula Sicurello > P Please consider the environment before printing this email. > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] silver stain for mouse intestinal nerves
Almost any method will work in intestine. I have had good results with Holmes' stain and more impressive results with Kiernan's method. I have also had good results with Winkelmann's method, but it is terribly time consuming. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Monday, January 12, 2009 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] silver stain for mouse intestinal nerves I am looking for a silver stain method that would stain nerves in intestines of mice. Helen Johnson (he...@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Silver staing for degenerating axons, thank you for sharing the paper.
A great thank you to the person who shared this paper. This was a very interesting read. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax ro...@baylorhealth.edu ** This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at priv...@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
