RE: [Histonet] Tape/Film Coverslips vs. Glass
The tape cover slippers are quicker, easier to manage the slides, but the cover slips pop off over time; the glass cover slippers are a bit temperamental; the slides take longer to dry, and the quality is better, and the cover slip stays on. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfie...@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Percival Karen Sent: Thursday, February 04, 2010 8:15 AM To: histonet@lists.utsouthwestern.edu; cathy.crump...@tuality.org; Pyse Cynthia Subject: RE: [Histonet] Tape/Film Coverslips vs. Glass I've used both over the years, and I love the tape coverslippers. The slides dry very quickly because there is no mounting media step. The tape sticks to the slide via a chemical reaction between the xylene and the tape. One thing to remember with tape, you cannot use recycled xylene as your last xylene step before coverslipping. If I remember correctly, you cannot use xylene substitutes either. It's been a while, so that may have changed. The tape coverslips are easily removed with acetone. The pathologists loved it because they received their slides immediately. We didn't have to allow drying time before handing them in. I didn't find any tissue fading over time, either. Just my opinion. Good luck. >>> "Cynthia Pyse" 2/3/2010 9:21 AM >>> Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tape/Film Coverslips vs. Glass
I've used both over the years, and I love the tape coverslippers. The slides dry very quickly because there is no mounting media step. The tape sticks to the slide via a chemical reaction between the xylene and the tape. One thing to remember with tape, you cannot use recycled xylene as your last xylene step before coverslipping. If I remember correctly, you cannot use xylene substitutes either. It's been a while, so that may have changed. The tape coverslips are easily removed with acetone. The pathologists loved it because they received their slides immediately. We didn't have to allow drying time before handing them in. I didn't find any tissue fading over time, either. Just my opinion. Good luck. >>> "Cynthia Pyse" 2/3/2010 9:21 AM >>> Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tape/Film Coverslips vs. Glass
Glass is generally better for photomicrotomy also. Pam Marcum UAMS - Original Message - From: "Cynthia Pyse" To: "Cathy Crumpton" , histonet@lists.utsouthwestern.edu Sent: Wednesday, February 3, 2010 8:21:15 AM GMT -06:00 US/Canada Central Subject: RE: [Histonet] Tape/Film Coverslips vs. Glass Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tape/Film Coverslips vs. Glass
Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?&nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet