RE: [Histonet] Fontana
What stain platform are you using? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, August 18, 2010 11:04 AM To: histonet Subject: [Histonet] Fontana Hello everone, We recently stained about 20 slides with Fontana for a research project. They were all fine except for 3 or 4 slides on which the pathologist says that the stratum corneum is overstained. The rest of the tissue is fine but that layer is too black. Now I have to fix the problem. Does anyone have an idea of why just that layer would overstain? On only a few slides of the set? Thank you! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] fontana
Any histological structure able to reduce silver into a black precipitate (argentaffin reaction) will be stained with the Fontana-Masson procedure. René J. --- On Wed, 2/24/10, Kalleberg, Kristopher wrote: From: Kalleberg, Kristopher Subject: [Histonet] fontana To: histonet@lists.utsouthwestern.edu Date: Wednesday, February 24, 2010, 9:18 AM Does anyone know of anything other than melanin that Fontana Masson would stain? Also, does anyone know of a stain that would label un/saturated fatty acids? Not sure if this is even possible. Thanks in advance. Kris Kalleberg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] fontana
Fontana-Masson also stains for Argentaffin granuals. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Wednesday, February 24, 2010 9:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fontana Does anyone know of anything other than melanin that Fontana Masson would stain? Also, does anyone know of a stain that would label un/saturated fatty acids? Not sure if this is even possible. Thanks in advance. Kris Kalleberg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fontana Masson
Try Poly Scientific Nicholas Rosenbaum HT, ASCP University of Virginia Medical Center From: "Putnam, Jodi" To: histonet@lists.utsouthwestern.edu Sent: Monday, April 27, 2009 12:03:11 PM Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fontana Masson
Rowley biochemical may also have it. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Putnam, Jodi Sent: Monday, April 27, 2009 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fontana Masson
I love the one from American Mastertech. www.americanmastertech.com Product code: KTFMA Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Putnam, Jodi Sent: Monday, April 27, 2009 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fontana Masson
Try Polyscientific R&D I think they have it. Pam Marcum UPENN Vet Sch New Bolton Center - Original Message - From: "Jodi Putnam" To: histonet@lists.utsouthwestern.edu Sent: Monday, April 27, 2009 12:03:11 PM GMT -05:00 US/Canada Eastern Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fontana Masson
Probably the best place to start is in Pearse's "Histochemistry" 4= th ed Vol 2 p.881 (1985). There is plenty of other interesting stuff= about melanin in the same chapter. There's also Lillie & Fu= llmer's "Histopathologic Technic ..." 4th ed p.522-527 (19= 76). Unfortunately neither book explains the chemistry of the argentaf= fin reaction of melanin. Pearse and Lillie assumed that their readers = knew about tests and reactions that had been in use for 100+ years! Unfo= rtunately this simple chemical information is not in many modern chemist= ry textbooks. older ones are more helpful. = You will need to follow up the references cited by Pearse and Lillie. If Unilever's library does not have these books, it's a =tinyinvestment(perhaps$200)for a huge company.= John KiernanAnatomy, UWOLond= on, Canada.= = =- Original Message -<= BR>From: "Kalleberg, Kristopher" <[EMAIL PROTECTED] NILEVER.COM>Date: Tuesday, December 2, 2008 15:04<= BR>Subject: [Histonet] Fontana MassonTo: [EMAIL PROTECTED] sts.utsouthwestern.edu> All,>= > Does anyone have a reference for me to read up on the = chemistry > involvedin Fontana Masson staining of melanin= ? I am having > a hard time finding> in = depth information on exactly howFMstainstains the > me= lanosmes and> the chemistry and reactions involved. Any information, >papers,or> references= would be greatly appreciated. Thank you in advance.>=> Kris Kalleberg>ResearchScientist> UnileverR&D> 40 Merritt Blvd.> Trumbull, CT 06611> (203) 381-5765&= gt; __ ___ 5F=_> Histonet mailing list> [EMAIL PROTECTED] ts.utsouthwestern.edu> http://lists.utsouthwester n.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet