Re: [Histonet] number of slides
Here is our basic microtomy protocol: BMT - 3 H&Es, each has a ribbon, plus PAS, Retic, Perls Liver bx - 3 H&Es, each has a ribbon plus Retic, Trichrome, Perls, Renal bx - 4 H&Es, each has a short ribbon plus PAS, PMS, trichrome - on slides with gloms Breast bx - 3 H&Es, each has a ribbon Derm bx - 4 H&Es, each has a ribbon, one Path likes AP PAS on all punch bx's GIT bx - 3 H&Es, each has a ribbon, plus HP all other small bx's get 3 H&Es, each with a ribbon and then we wait for the orders for levels and deepers and..and...and AbuDhabiAnnie On 25 March 2010 00:19, hymclab wrote: > We follow the same practice as Susan. Our Pathologists would rather have > more than less!!! > > Dawn > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue > Sent: Tuesday, March 23, 2010 5:08 PM > To: anita dudley > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] number of slides > > We do three levels on all diagnostic biopsies. Liver and Kidney also get > upfront special stains, as do gastric biopsies (h pylori) We did cut down on > extra unstained slides since we were discarding most of them. As far as > stopping levels, my pathologist thinks it goes against standard of care. As > for prostate biopsies, they are so small any more, that we are thinking o > cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible > IHC. We are finding that when we have to go back there is minimal tumor for > IHC demonstration. > > Susan T. Paturzo > Thomas Jefferson University Hospital > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the > designated recipient(s) named above. If you are not the intended recipient, > you are hereby notified that you have received this communication in error > and that any review, disclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication in > error, please notify the sender at the electronic mail address noted above > and destroy all copies of this communication and any attachments. Thank you > for your cooperation. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] number of slides
We follow the same practice as Susan. Our Pathologists would rather have more than less!!! Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 23, 2010 5:08 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] number of slides We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] number of slides
We basically do the same at Tom's lab, the only exception is we file the unstained slides with the stained slides. Especially with the FNA biopsies. We have had to pull the unstained slide to send out for IHC's. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar [tmcne...@lmhealth.org] Sent: Wednesday, March 24, 2010 6:11 AM To: Sue; anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] number of slides We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone marrows. We also do special stains up front on the bone marrows, H Pyloris, prostates, etc. We tend to cut a ton of extra slides that we just throw away. I don't like it but I don't think there's any way around it. Probably like most places, we get more and more smaller and smaller biopsies so it's better to keep a few extras than to try to go back later. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 23, 2010 6:08 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] number of slides We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4061. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] number of slides
We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone marrows. We also do special stains up front on the bone marrows, H Pyloris, prostates, etc. We tend to cut a ton of extra slides that we just throw away. I don't like it but I don't think there's any way around it. Probably like most places, we get more and more smaller and smaller biopsies so it's better to keep a few extras than to try to go back later. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 23, 2010 6:08 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] number of slides We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4061. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] number of slides
Routinely Gastric bx upper / rt colon / lt colon one rubbon of 4 sections per slides Liver HE @ level 1/2/3 and liver specials, AE1 IHC Renal Biopsy (Native and Transplant) 24 slides inc HE/Renal Special/ IHC and USS TransBronchial BX HE @ level 1/2/3 and lung special BMT HE, retic and IHC for NBLX case Endomyocardial BX, HE @level 1/2/3 EVG/MT and C4d IHC Vascular Malformation HE and IHC Surgical Heart (11 blocks) HE, EVG on all Blocks Cornea Button HE, PAS, AB, Congo red All Tumour BX HE, and 10 USS for IHC PM Brain HE/LFB on all blocks PM Heart HE/EVG on all blocks PM Lung HE/EVG/PERLS on all blocks Working in paediatric I have not seen a prostate of a breast for the past 6 years, but my old lab had between 4 and 6 blocks per prostate or breast tissue and were cutting HE at level 1/2/3 and picking up 4 USS between level for further IHC. Malika Malika Benatti Specialist Biomedical Scientist Great Ormond Street Hospital for Children On Tue, Mar 23, 2010 at 10:08 PM, Sue wrote: > We do three levels on all diagnostic biopsies. Liver and Kidney also get > upfront special stains, as do gastric biopsies (h pylori) > We did cut down on extra unstained slides since we were discarding most of > them. As far as stopping levels, my pathologist thinks it goes against > standard > of care. As for prostate biopsies, they are so small any more, that we are > thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for > possible IHC. We > are finding that when we have to go back there is minimal tumor for IHC > demonstration. > > Susan T. Paturzo > Thomas Jefferson University Hospital > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- " Smile it confuses people " ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] number of slides
We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet