Re: [Histonet] Eosin on processor for biopsies

[Bob Richmond via Histonet]

Gareth Davis asked about marking small GI specimens with dye when grossing
them.

I've used safranin O - the solution the microbiologists use in the Gram
stain. If you walk down the hall to the micro lab you can get a small
amount to try out.

Do not use eosin for this purpose. Eosin's brilliant fluorescence makes it
very difficult to do any fluorescent stain (such as FISH) on the sections.
Safranin O isn't fluorescent. It also works a lot better than eosin.

Equally important is to log how many specimens you put in the cassette when
you're grossing, and to have that log in front of you when you embed. (I've
had a lot of histotechs flatly refuse to do this.)

I favor those little blue foam pads to put small specimens on. I usually
cut them in two, so that I use a total of only one in each cassette.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] Eosin on processor for biopsies

[Blazek, Linda via Histonet]

We have put eosin in the last alcohol on the processor for years.  It stays in 
the esophageal biopsies just fine.  
Linda


-Original Message-
From: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, January 10, 2019 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin on processor for biopsies

Gareth,

reguarding the eosin biopsy post on the histo net. Things you might want to 
considered looking at, I cannot say for sure because I don't know your 
processing solutions/schedule:

Most Eosins are alcohol soluable, by putting the eosin in your formalin most of 
it will wash out during processing. Many labs put the Eosin in the last alcohol.

Hematoxylin is typically water soluable, if you put it in formailin processing 
usually removes water if the formailin is followed by alcohols.

I hope this helps,

Cassie


From: Gareth Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 10 January 2019 9:15 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Opinion on dye on biopsies

So, I work in a small GI lab, and I put Eosin in my first formalin on my 
processor.  My biopsies are very small and this helps, somewhat, to see the 
specimens for embedding and cutting.  But, unfortunately, the esophagus tissues 
do not absorb the eosin much.  Anyway, the hospital lab I work, part-time, in 
has started using hematoxylin to help see their biopsies.  I happen to embed 
there and I think it just makes a big mess and the tissue does not absorb much 
of the stain.
What are other labs doing to aid in making their small biopsies easier to see?  
What are pros and cons to doing this, in your opinion?
Thanks!

--
*Ms. Gareth B. Davis*, B.S., HT, QIHC  (ASCP)cm Yuma Gastroenterology Yuma, AZ 
85364
928-248-5259
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Re: [Histonet] Eosin

[Bob Richmond via Histonet]

Elizabeth M. Cameron, HT(ASCP), QIHCCM. Lead Histologist at Mid Coast
Hospital in Brunswick, Maine asks:

>>I have been staining fish tissues fixed in Davidson's fixative with H&E,
and the researcher would like the eosin to be more intense. Our standard
protocol works well for our own tissue, but the fish look much more washed
out. I am using alcoholic eosin Y, have tried both water and alcohol before
and I have varied the alcohol differentiation steps after the Eosin. I also
extended the time in Eosin and increased the wash after bluing to make sure
the sections are not basic. Any suggestions would be appreciated.<<

Users vary a lot in how much eosin they want. Sometimes blends with other
red dyes are preferable. When I was a resident at Johns Hopkins around 1970
our notoriously lurid eosin was compounded as follows:

Eosin Y (C.I. 45380) 3.6 g

Phloxine B (C.I. 45410) 1.5 g

Biebrich scarlet (C.I. 26905 ) 0.3 g

absolute alcohol  150   mL

distilled water   120   mL

Shake, or stir with a magnetic stirrer to dissolve, then add 450 mL more of
distilled water.

We used a lot of Davidson's fixative at JHH. As far as I know this formula
was never published, though I've posted it online more than once, probably
on Histonet. One of many formulas I made off with before I finished
residency.

Bob Richmond

Samurai Pathologist

Maryville TN
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Re: [Histonet] Eosin

[Debbie Faichney via Histonet]

Hi Elizabeth,

We are an aquaculture histology laboratory and routinely use four parts 1% 
Eosin Y (aq) to one part Putts eosin for the same reason that you describe.  1% 
aqueous or alcoholic alone just doesn't do the job.

Debbie Faichney, BSc

Senior Technician
Histology/Bacteriology Laboratories
Institute of Aquaculture
University of Stirling
Stirling, FK9 4LA
Scotland
UK








-Original Message-
From: Cameron, Elizabeth via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: 30 August 2016 15:25
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin

Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the 
researcher would like the eosin to be more intense.  Our standard protocol 
works well for our own tissue, but the fish look much more washed out.  I am 
using alcoholic eosin Y, have tried both water and alcohol before and I have 
varied the alcohol differentiation steps after the Eosin.  I also extended the 
time in Eosin and increased the wash after bluing to make sure the sections are 
not basic.  Any suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573

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Re: [Histonet] Eosin

[E . Wayne Johnson 朱稳森博士 via Histonet]

You might try some combination of eosin and biebrich scarlet and/or 
phloxine.


Adding a little acetic acid might help.

http://www.stainsfile.info/StainsFile/downloads/eosin.pdf

I am quite fond of Biebrich Scarlet and like way it stains brain and 
heart particularly.


Biebrich is much more red than eosin.

Phloxine is an aggressive pink.

*

A bit punny about the fish looking washed out.

*

"There was something fishy about the butler.

Probably a Pisces working for scale." - Phil Proctor



On 08/30/2016 10:25 PM, Cameron, Elizabeth wrote:

Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the 
researcher would like the eosin to be more intense.  Our standard protocol works 
well for our own tissue, but the fish look much more washed out.  I am using 
alcoholic eosin Y, have tried both water and alcohol before and I have varied the 
alcohol differentiation steps after the Eosin.  I also extended the time in Eosin 
and increased the wash after bluing to make sure the sections are not basic.  Any 
suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573




--
E. Wayne Johnson 朱稳森博士
Enable AgTech Consulting
恩睿康农业技术咨询有限公司
Beijing
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Re: [Histonet] Eosin

[Jennifer MacDonald via Histonet]

Elizabeth,
You can try to lower the pH of the eosin a bit.  Add some acetic acid. Use 
absolute alcohol after the eosin for differentiating/dehydrating.
Jennifer



From:   "Cameron, Elizabeth via Histonet" 

To: "histonet@lists.utsouthwestern.edu" 

Date:   08/30/2016 07:29 AM
Subject:[Histonet] Eosin



Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the 
researcher would like the eosin to be more intense.  Our standard protocol 
works well for our own tissue, but the fish look much more washed out.  I 
am using alcoholic eosin Y, have tried both water and alcohol before and I 
have varied the alcohol differentiation steps after the Eosin.  I also 
extended the time in Eosin and increased the wash after bluing to make 
sure the sections are not basic.  Any suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573

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Re: [Histonet] Eosin tissue marker on Peloris II

[Bob Richmond via Histonet]

Jim Vickroy, Histology Manager, Springfield Clinic, Springfield, Illinois
asks:

>>Someone told me that you could not use eosin on the new Peloris II. Many
of us use eosin to color small biopsies during the process run. We have it
in one of the 95% ETOHs. Can someone explain why this can't be used in the
Peoris?<<

You shouldn't be coloring small biopsy specimens with eosin on any
processor, because eosin's brilliant fluorescence interferes with FISH
procedures (such as HER2 on breast cancers). If you want to use a red
marking dye, use safranin O. The solution the microbiologists use in Gram
stains works quite well, and is probably just across the hallway for you.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] Eosin

[Monson, Frederick via Histonet]

Long ago, in the foggy, foggy past,  we were admonished to insure that our 
Eosin in the bottle had a slight 'green' tinge in good daylight.

When we finally got fluorescence, we would check an H&E-stained section with 
the fluorescein filters to insure the fluorescence of the Eosin. 

Very scientific, I know, but when the 'green' was gone, the Eosin was replaced.

Cheers,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pennsylvania
Schmucker Science South - Room SSS-024
MailDrop:  Geology-Astronomy
750 South Church Street
West Chester, PA, 19383
610-738-0437
fmon...@wcupa.edu

-Original Message-
From: Kienitz, Kari [mailto:kkien...@orclinic.com] 
Sent: Monday, July 13, 2015 11:21 AM
To: Hannen, Valerie; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin

It's always kind of a stab in the dark to diagnose H&E problems without a 
little more information.  One thing I would try if you don't feel its an actual 
Eosin problem is your alcohol prior to the eosin.  Slide volume and humidity 
can dilute your alcohol and cause lighter cytoplasmic staining.  The alcohol 
prior to eosin should be 95% and changed daily if need be.


Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
 NE 99th Ave
Portland, OR  97220
503.935.8311
kkien...@orclinic.com




CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive 
and confidential use of the intended recipient. If you are not the intended 
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missive. If you have received this in error, please notify the sender 
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From: Hannen, Valerie [valerie.han...@parrishmed.com]
Sent: Monday, July 13, 2015 8:05 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin

Good morning,

I once again am dealing with a my picky Pathologist!!  About a year ago, he 
started to complain about not having enough Eosin on his sections( first 
morning rack on Monday).. I went from changing the Eosin on Friday and stirring 
it on Monday to totally changing it  on Monday...it has all been good until the 
end of last week. The problem has started up again... little Eosin in the first 
rack of Monday morning.  Any suggestions??

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com>
www.parrishmed.com

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Re: [Histonet] Eosin

[Rene J Buesa]

Sometimes these issues are "mysterious" and in your case are particularly 
"mysterious" because you once solved the problem, only to recently "reappear".I 
am going to tell you how I used to do it and never had those PT rejections:1- 
for both hematoxylin and eosin I kept a log of the number of stained slides and 
changes both when I reached 200, regardless of the day of the week or the time 
within the day the amount was reached.2- I always used the same brand (Harleco) 
3- I never left the staining set over the week. Everything was removed the last 
day of work (Saturday) and the containers were rinsed and dried and left upside 
down. For the automatic stainer (Sakura) we did the same thing.4- come Monday 
morning the whole set was prepared and everything started again.
Now, before I retired all the method was changed and this part probably you 
will not attempt: we dewaxed with dishwasher soap (elimination of xylene and 
ethanol). The slides went directly to the hematoxylin → bluing → 
differentiation → water → eosin → wash water and from then directly to an oven 
at 60ºC for 15 minutes (elimination of ethanol and xylene) → coverslip
So, try as I describe in points 1 to 4 or you can even could try eliminating 
xylene and ethanol and "go green" and "dry".René 


 On Monday, July 13, 2015 1:30 PM, "Hannen, Valerie" 
 wrote:
   

 #yiv6464311563 #yiv6464311563 -- _filtered #yiv6464311563 
{font-family:Helvetica;panose-1:2 11 6 4 2 2 2 2 2 4;} _filtered #yiv6464311563 
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{margin:1.0in 1.0in 1.0in 1.0in;}#yiv6464311563 div.yiv6464311563WordSection1 
{}#yiv6464311563 Rene,  As I stated before and maybe I was not entirely clear, 
we had this problem ( little Eosin in the sections of the first rack of slides 
on Monday morning) before, but had remedied it by starting to change the Eosin 
on Monday morning before the first rack of slides was run.  He now is stating 
that the problem of little Eosin in the sections from the first rack on Monday 
A.M. is starting again.  Since the last fix of the problem we have not deviated 
in any way, procedure or chemical wise.  We have used the same lot # in 
previous weeks for the Eosin as we currently have on the stainer ( I changed 
the Eosin this morning) , so I don’t think it is an Eosin problem per se.  
Kari,  We do use 95% alcohol before our Eosin at all times.  I would think that 
if the alcohol before the  Eosin was the problem, all of my racks would have 
the “little Eosin in the sections” problem.. not just the first rack.  Again 
any and all suggestions are welcomed!!  Thanks again Rene and Kari for 
replying.  Valerie  From: Rene J Buesa [mailto:rjbu...@yahoo.com] 
Sent: Monday, July 13, 2015 11:20 AM
To: Hannen, Valerie; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin  It is difficult to advise you anything without 
knowing what is your PT's complaint. What he does complain about?
René    On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie" 
 wrote:  Good morning,

I once again am dealing with a my picky Pathologist!!  About a year ago, he 
started to complain about not having enough Eosin on his sections( first 
morning rack on Monday).. I went from changing the Eosin on Friday and stirring 
it on Monday to totally changing it  on Monday...it has all been good until the 
end of last week. The problem has started up again... little Eosin in the first 
rack of Monday morning.  Any suggestions??

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com>
www.parrishmed.com

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Re: [Histonet] Eosin

[Hannen, Valerie]

Rene,

As I stated before and maybe I was not entirely clear, we had this problem ( 
little Eosin in the sections of the first rack of slides on Monday morning) 
before, but had remedied it by starting to change the Eosin on Monday morning 
before the first rack of slides was run.  He now is stating that the problem of 
little Eosin in the sections from the first rack on Monday A.M. is starting 
again.  Since the last fix of the problem we have not deviated in any way, 
procedure or chemical wise.  We have used the same lot # in previous weeks for 
the Eosin as we currently have on the stainer ( I changed the Eosin this 
morning) , so I don’t think it is an Eosin problem per se.

Kari,

We do use 95% alcohol before our Eosin at all times.  I would think that if the 
alcohol before the  Eosin was the problem, all of my racks would have the 
“little Eosin in the sections” problem.. not just the first rack.

Again any and all suggestions are welcomed!!

Thanks again Rene and Kari for replying.

Valerie

From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Monday, July 13, 2015 11:20 AM
To: Hannen, Valerie; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin

It is difficult to advise you anything without knowing what is your PT's 
complaint. What he does complain about?
René


On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie" 
mailto:valerie.han...@parrishmed.com>> wrote:

Good morning,

I once again am dealing with a my picky Pathologist!!  About a year ago, he 
started to complain about not having enough Eosin on his sections( first 
morning rack on Monday).. I went from changing the Eosin on Friday and stirring 
it on Monday to totally changing it  on Monday...it has all been good until the 
end of last week. The problem has started up again... little Eosin in the first 
rack of Monday morning.  Any suggestions??

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com><mailto:valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com>>
www.parrishmed.com<http://www.parrishmed.com>

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Re: [Histonet] Eosin

[Kienitz, Kari]

It's always kind of a stab in the dark to diagnose H&E problems without a 
little more information.  One thing I would try if you don't feel its an actual 
Eosin problem is your alcohol prior to the eosin.  Slide volume and humidity 
can dilute your alcohol and cause lighter cytoplasmic staining.  The alcohol 
prior to eosin should be 95% and changed daily if need be.


Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
 NE 99th Ave
Portland, OR  97220
503.935.8311
kkien...@orclinic.com




CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive 
and confidential use of the intended recipient. If you are not the intended 
recipient, please do not read, distribute or take action in reliance upon this 
missive. If you have received this in error, please notify the sender 
immediately by reply e-mail and delete this message and its attachments from 
your computer system. Thank you

From: Hannen, Valerie [valerie.han...@parrishmed.com]
Sent: Monday, July 13, 2015 8:05 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin

Good morning,

I once again am dealing with a my picky Pathologist!!  About a year ago, he 
started to complain about not having enough Eosin on his sections( first 
morning rack on Monday).. I went from changing the Eosin on Friday and stirring 
it on Monday to totally changing it  on Monday...it has all been good until the 
end of last week. The problem has started up again... little Eosin in the first 
rack of Monday morning.  Any suggestions??

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com

==
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Re: [Histonet] Eosin

[Rene J Buesa]

It is difficult to advise you anything without knowing what is your PT's 
complaint. What he does complain about?
René 


 On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie" 
 wrote:
   

 Good morning,

I once again am dealing with a my picky Pathologist!!  About a year ago, he 
started to complain about not having enough Eosin on his sections( first 
morning rack on Monday).. I went from changing the Eosin on Friday and stirring 
it on Monday to totally changing it  on Monday...it has all been good until the 
end of last week. The problem has started up again... little Eosin in the first 
rack of Monday morning.  Any suggestions??

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com

==
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Re: [Histonet] Eosin pH

[John Kiernan]

Dissolve it in a buffer (eg acetic acid-sodium acetate) at or near pH 4.5.
John Kiernan
London, Canada
= = =
On 23/04/15, Pablo Sanchez-Quinteiro   wrote:
> Listers, Could you tell me the best way to adjut the eosin pH to 4-5?
> 
> Thanks in advance
> 
> Pablo 
> 
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RE: [Histonet] Eosin Leaching

[Michael LaFriniere]

I have seen this happen with high levels of Isopropanol in alcohols after the 
eosins, I switch to regular reagent alcohols  and seemed to eliminate the 
bleeding of the eosin.

Michael
Michael R. LaFriniere, HT (ASCP) 
Executive Director
 

Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A * Silver Spring, MD 20904  
P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844
michael.lafrini...@ccplab.com
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read 
that this can be caused by not dehydrating adequately after eosin, but we're 
still having an issue when using fresh alcohols. Does anyone have any other 
ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

This message, including any attachments, is confidential and may be privileged 
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RE: [Histonet] Eosin Leaching

[Tony Reilly]

Hi Adrienne

Also make sure the wash after your bluing agent is higher than the blueing 
solution or alkalinity of the solution will leach out your eosin.

Regards
Tony


Tony Reilly B.App.Sc,  M.Sc
Chief Scientist
Anatomical Pathology
Pathology Queensland PAH
_
Health Services Support Agency| Department of Health

Building 15, Level 1, 
199 Ipswich Road 
WOOLLOONGABBA  Queensland 4102
Ph: 07 3176 2412
Mob: 0402139411
Fax: 07 3176 2930
Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au 
     





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, 20 June 2014 10:11 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 
'histo...@listsutsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Is your alcohol level as high as or higher than the level of eosin in the 
containers? If there is residual eosin left at the upper portion of the slide, 
that could be causing the problem.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Have you changed your coverslipping mountant?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read 
that this can be caused by not dehydrating adequately after eosin, but we're 
still having an issue when using fresh alcohols. Does anyone have any other 
ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

This message, including any attachments, is confidential and may be privileged 
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RE: [Histonet] Eosin Leaching

[Vanessa Avalos]

We had the same problem. Increase your Alcohol/Reagents (following Eosin) times 
as well as using fresh ones. I use 80% for about 20 sec and (2)100% for a 
minute each and (3) Xylene Subs for 2 min each. I also use a fresh 80% and 
rotate the 100% each day. If using a Xylene Sub it could also be a possibility 
that your mounting medium is not compatible. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Thursday, June 19, 2014 2:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read 
that this can be caused by not dehydrating adequately after eosin, but we're 
still having an issue when using fresh alcohols. Does anyone have any other 
ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

This message, including any attachments, is confidential and may be privileged 
or may contain health information protected by state and federal law.
Information and opinions expressed in this message and/or attachments are those 
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RE: [Histonet] Eosin Leaching

[Anita Buchiane]

I'd advise checking your dehydration alcohols with a hydrometer.  This 
"bleeding" happened to me once and it turned out the alcohols after the Eosin 
were not graded.  My lab aide accidently used 100% only instead of 70%, 95% and 
then 100%.  
Anita

Anita

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Friday, June 20, 2014 8:15 AM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin Leaching

We haven't changed our mounting medium, but I'll have to check on the alcohol 
levels. When I stain (we hand stain only - very small lab), I always make sure 
the alcohol is higher than the eosin, but I have a lab assistant now, and it's 
very possible I didn't make that a point. I'll try that.

Any more tips are welcome still - thank you all!

Adrienne


On Jun 20, 2014, at 8:11 AM, Rathborne, Toni  wrote:

> Is your alcohol level as high as or higher than the level of eosin in the 
> containers? If there is residual eosin left at the upper portion of the 
> slide, that could be causing the problem.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
> Jeanine (CDC/OID/NCEZID)
> Sent: Thursday, June 19, 2014 5:26 PM
> To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
> Subject: RE: [Histonet] Eosin Leaching
> 
> Have you changed your coverslipping mountant?
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
> Anderson
> Sent: Thursday, June 19, 2014 5:24 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Eosin Leaching
> 
> Hello all,
> 
> We're having a problem with our eosin bleeding out of the sections. I've read 
> that this can be caused by not dehydrating adequately after eosin, but we're 
> still having an issue when using fresh alcohols. Does anyone have any other 
> ideas as to the cause of this problem?
> 
> Thanks in advance for your help!
> 
> Adrienne
> 
> 
> 
> Adrienne Anderson, BS, HTL(ASCP)
> Histotechnologist
> Phylogeny, Inc. 
> 1476 Manning Pkwy, Powell, Ohio 43605
> Phone: (614) 846-6161 
> Fax:  (877) 591-1815
> 
> This message, including any attachments, is confidential and may be 
> privileged or may contain health information protected by state and federal 
> law.
> Information and opinions expressed in this message and/or attachments are 
> those of the author and are not necessarily those of the company. 
> If you are not the intended recipient, please notify the sender and delete 
> this message from your system. 
> Any use of this information by individuals other than the intended recipient 
> is strictly prohibited. ___
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Re: [Histonet] Eosin Leaching

[Adrienne Anderson]

We haven’t changed our mounting medium, but I’ll have to check on the alcohol 
levels. When I stain (we hand stain only - very small lab), I always make sure 
the alcohol is higher than the eosin, but I have a lab assistant now, and it’s 
very possible I didn’t make that a point. I’ll try that.

Any more tips are welcome still - thank you all!

Adrienne


On Jun 20, 2014, at 8:11 AM, Rathborne, Toni  wrote:

> Is your alcohol level as high as or higher than the level of eosin in the 
> containers? If there is residual eosin left at the upper portion of the 
> slide, that could be causing the problem.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
> Jeanine (CDC/OID/NCEZID)
> Sent: Thursday, June 19, 2014 5:26 PM
> To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
> Subject: RE: [Histonet] Eosin Leaching
> 
> Have you changed your coverslipping mountant?
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
> Anderson
> Sent: Thursday, June 19, 2014 5:24 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Eosin Leaching
> 
> Hello all,
> 
> We're having a problem with our eosin bleeding out of the sections. I've read 
> that this can be caused by not dehydrating adequately after eosin, but we're 
> still having an issue when using fresh alcohols. Does anyone have any other 
> ideas as to the cause of this problem?
> 
> Thanks in advance for your help!
> 
> Adrienne
> 
> 
> 
> Adrienne Anderson, BS, HTL(ASCP)
> Histotechnologist
> Phylogeny, Inc. 
> 1476 Manning Pkwy, Powell, Ohio 43605
> Phone: (614) 846-6161 
> Fax:  (877) 591-1815
> 
> This message, including any attachments, is confidential and may be 
> privileged or may contain health information protected by state and federal 
> law.
> Information and opinions expressed in this message and/or attachments are 
> those of the author and are not necessarily those of the company. 
> If you are not the intended recipient, please notify the sender and delete 
> this message from your system. 
> Any use of this information by individuals other than the intended recipient 
> is strictly prohibited. ___
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RE: [Histonet] Eosin Leaching

[Rathborne, Toni]

Is your alcohol level as high as or higher than the level of eosin in the 
containers? If there is residual eosin left at the upper portion of the slide, 
that could be causing the problem.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching

Have you changed your coverslipping mountant?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read 
that this can be caused by not dehydrating adequately after eosin, but we're 
still having an issue when using fresh alcohols. Does anyone have any other 
ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

This message, including any attachments, is confidential and may be privileged 
or may contain health information protected by state and federal law.
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RE: [Histonet] Eosin Leaching

[Sanders, Jeanine (CDC/OID/NCEZID)]

Have you changed your coverslipping mountant?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read 
that this can be caused by not dehydrating adequately after eosin, but we're 
still having an issue when using fresh alcohols. Does anyone have any other 
ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

This message, including any attachments, is confidential and may be privileged 
or may contain health information protected by state and federal law.
Information and opinions expressed in this message and/or attachments are those 
of the author and are not necessarily those of the company. 
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Re: [Histonet] Eosin problem

[Rene J Buesa]

Firstly: you should not schedule changing the eosin or any stain for that 
matter on a "time" (in this case) basis. The reagents ought to be changed on 
usage basis, i.e. establish a maximum slides to be stained before changing the 
reagents.
Second: your pathologists are wrong because had the time in formalin any 
bearing on the problem, all the racks should come pale and not only the first.
Third: it is very likely that during the weekend some sort of surface oxidation 
may occur and that is why stirring the eosin "solves" the problem.
Four: Faced with this problem and if you insist on changing the reagents of a 
time basis, change them on Mondays even if somebody has to come on the weekend 
to section/stan
René J.



From: "Hannen, Valerie" 
To: "Histonet Post (histonet@lists.utsouthwestern.edu)" 
 
Sent: Tuesday, September 3, 2013 1:09 PM
Subject: [Histonet] Eosin problem


Hi All,'

Hoping someone can give me some insight on if this happens/ happened to them.  
We change all of our reagents on the H&E stainer on Friday( which means the 
reagents sit idle over the weekend.)  We rarely have to come in on the weekends 
due to having to embed/cut/stain tissues.  However, on Monday or in this case 
today (Tues because of the Holiday), we have to stir our Eosin before putting 
our first rack through for staining because the Pathologists have been 
complained that the Eosin is too light if we don't.  But I've had one of them 
tell me that a second and subsequent rack of slides does'nt have this problem.  
The Pathologist wants to know if by the tissue sitting in Formalin longer over 
the weekend on the processor vs. only sitting a few extra hours on the 
overnight run, is the extra time in Formalin causing this problem?
I told him that I did'nt think so, but that I would ask.  I also thought that 
maybe in addition to stirring the Eosin, maybe running a rack of "blank" slides 
through a staining program before puttng the patient tissue through might help. 
 What do you all think??


Thanks so much!!


Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com



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RE: [Histonet] eosin in the processor

[Davis, Cassie]

Hi Kim, 

try 10cc of the prediluted Eosin (you use in H & E staining) in your last 100% 
before your Xylene on the processor. By putting it in the last absolute it will 
not wash out. Your first Xylene will end up a little pink but your eyes will 
not strain as much trying to fine the tiny tissue fragments. (great for 
thread-like prostate biopsies too) We had been using it for years & our IHC 
seems fine. Make sure to keep your solutions for the clean cycles maintenanced 
on the automatic processors to avoid clogging. The only reason we stopped was 
the folks we purchased the new processor from told us using it would invalidate 
the warranty on the new processor.(I really miss it)

Cassandra Davis
cda...@che-east.org
302-575-8095


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Kim Merriam 
[kmerriam2...@yahoo.com]
Sent: Wednesday, November 28, 2012 7:54 AM
To: Histonet
Subject: [Histonet] eosin in the processor
Hi Everyone,
Years ago, my lab used to put eosin in the processor to lightly tint the 
smaller mouse tissues.  I can't remember which station we put it in (I think it 
was the 2nd 100% ethanol).  Also, back then my lab didn't do any IHC; will the 
eosin affect any IHC that might be done (I am guessing no, but I want to be 
sure).
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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RE: [Histonet] eosin in the processor

[Monfils, Paul]

Eosin won't interfere with binding of antibodies, but eosin is
fluorescent.  You might want to consider that.



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RE: [Histonet] eosin in the processor

[gayle callis]

The only immunostaining any residual eosin in tissue might affect is if you
do immunofluorescence.  Eosin does fluoresce.   Others can address any
effect on IHC.  

Gayle Callis
HTL/HT/MT(ASCP)
Bozeman  MT 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Wednesday, November 28, 2012 5:55 AM
To: Histonet
Subject: [Histonet] eosin in the processor

Hi Everyone,

Years ago, my lab used to put eosin in the processor to lightly tint the
smaller mouse tissues.  I can't remember which station we put it in (I think
it was the 2nd 100% ethanol).  Also, back then my lab didn't do any IHC;
will the eosin affect any IHC that might be done (I am guessing no, but I
want to be sure).

Thanks,
Kim
 
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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RE: [Histonet] eosin in the processor

[Lynette Pavelich]

Of our 3- 100% ETOH on our processor, we put about 75mL of LIQUID eosin in our 
"dirtiest" 100%. Our purchased eosin is made with 95% alcohol, and it works 
very well, not affecting the processing.

We used to use about 1/4 tsp of Eosin Y powder in our "dirtiest" 100%, but as 
it needs some water to dissolve, it caused trouble in the valves of the 
processor (oops). We tried using it in our 95%, but it got too washed out by 
the end of processing. 

No ill effects on our IHC.

Lynette



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Kim Merriam 
[kmerriam2...@yahoo.com]
Sent: Wednesday, November 28, 2012 7:54 AM
To: Histonet
Subject: [Histonet] eosin in the processor

Hi Everyone,

Years ago, my lab used to put eosin in the processor to lightly tint the 
smaller mouse tissues.  I can't remember which station we put it in (I think it 
was the 2nd 100% ethanol).  Also, back then my lab didn't do any IHC; will the 
eosin affect any IHC that might be done (I am guessing no, but I want to be 
sure).

Thanks,
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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Re: [Histonet] Eosin

[Brendal Finlay]

Do you recycle your alcohols?  If you do and you put the recycled
alcohols at the end (running down to xylene) it can GREATLY decolorize
the eosin in the H&E stain.  Also, make sure you have a good rinse
after the blueing step as that can mess with the eosin's pH which will
also decrease staining.



-Original message-
From: "Hannen, Valerie" valerie.han...@parrishmed.com
Date: Thu, 09 Aug 2012 09:23:26 -0500
To: histonet histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin

> Hi Folks...I am hoping you all give me a little help. Our
Pathologists are complaining about our Eosin on the H&E's being weak.
The funny thing is, is that it can go one
> 
> day to the next...one day it looks great...the next it is weak!!
> 
> I have already done some experimenting with...1) time tissue spends
in Eosin 2) making sure that the alcohols after Eosin are the
properconcentrations3)
> 
> reducing the time that the tissues spends in the alcohols atfer
Eosin... I have even gone as far as 4) increasing the rinse time in
water after the decolorizing and bluing
> 
> steps. 5) I have checked the pH of the water as well.
> 
> Any help and suggestions would greatly appreciated!!
> 
> Thanks Gang!!
> 
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.han...@parrishmed.com
> 
> 
> 
> 
> 
> 
> 
> *
> 
> 
> "This email is intended solely for the use of the individual to
> whom it is addressed and may contain information that is
> privileged, confidentialor otherwise exempt from disclosure
> under applicable law. If the reader of this email is not the
> intended recipient or the employee or agent responsible for
> delivering the message to the intended recipient, you are
> hereby notified that any dissemination, distribution, or
> copying of this communication is strictly prohibited. If you
> have received this communication in error, please immediately
> delete this message. Thank you"
> 
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

Brendal C. Finlay, HT (ASCP)
West Florida Medical Center Clinic
brendal.fin...@medicalcenterclinic.com
phone - 850.474.8758
fax - 850.474.8584

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Re: [Histonet] Eosin

[Geoff McAuliffe]

Add a few drops of glacial acetic acid.

Geoff

On 8/9/2012 11:23 AM, Hannen, Valerie wrote:

Hi Folks...I am hoping you all give me a little help.  Our Pathologists are 
complaining about our Eosin on the H&E's being weak. The funny thing is, is 
that it can go one

day to the next...one day it looks great...the next it is weak!!

I have already done some experimenting with...1) time tissue spends in 
Eosin 2) making sure that the  alcohols after Eosin are the proper 
concentrations3)

  reducing the time that the tissues spends in the alcohols atfer Eosin... I 
have even gone as far as 4) increasing the rinse time in water after the 
decolorizing and bluing

  steps. 5) I have checked the pH of the water as well.

Any help and suggestions would greatly appreciated!!

Thanks Gang!!

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com







*

==
=

"This email is intended solely for the use of the individual to
whom it is addressed and may contain information that is
privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
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have received this communication in error, please immediately
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--
--
**
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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Re: [Histonet] Eosin

[Jennifer Campbell]

What clearing agent are you using?

If it is a xylene substitute then you might want to rotate or change the
alcohols after eosin after a few racks of slides.

We use ProPar on our stainer and rotate our alcohols after eosin after
every 3 racks that have gone through.



On Thu, Aug 9, 2012 at 11:23 AM, Hannen, Valerie <
valerie.han...@parrishmed.com> wrote:

> Hi Folks...I am hoping you all give me a little help.  Our Pathologists
> are complaining about our Eosin on the H&E's being weak. The funny thing
> is, is that it can go one
>
> day to the next...one day it looks great...the next it is weak!!
>
> I have already done some experimenting with...1) time tissue spends in
> Eosin 2) making sure that the  alcohols after Eosin are the proper
> concentrations3)
>
>  reducing the time that the tissues spends in the alcohols atfer Eosin...
> I have even gone as far as 4) increasing the rinse time in water after the
> decolorizing and bluing
>
>  steps. 5) I have checked the pH of the water as well.
>
> Any help and suggestions would greatly appreciated!!
>
> Thanks Gang!!
>
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.han...@parrishmed.com
>
>
>
>
>
>
>
> *
>
>
> "This email is intended solely for the use of the individual to
> whom it is addressed and may contain information that is
> privileged, confidential or otherwise exempt from disclosure
> under applicable law. If the reader of this email is not the
> intended recipient or the employee or agent responsible for
> delivering the message to the intended recipient, you are
> hereby notified that any dissemination, distribution, or
> copying of this communication is strictly prohibited. If you
> have received this communication in error, please immediately
> delete this message. Thank you"
>
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Jen Campbell, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137


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RE: [Histonet] Eosin staining for small biopsies

[Cynthia Pyse]

We also use safranin on all our small biopsies and also our breast biopsies
with no adverse effects to any of our IHC or ISH.
Cindy

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cp...@x-celllab.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia
Robinson
Sent: Wednesday, June 20, 2012 10:32 AM
To: histonet@lists.utsouthwestern.edu; Carol Freeman
Subject: Re: [Histonet] Eosin staining for small biopsies

We use safranin (used in Microbiology as a counterstain)  on our small
biopsies. We apply a small drop during grossing. It does not affect staining
of H&E, IHC or ISH. We like this because it is an intense red that doesn't
leach out in the alcohols of processor.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


>>> "Freeman, Carol"  6/20/2012 8:46 AM >>>


Good Morning all, Happy Wednesday!

I have a question and I am not finding much on my google search...First does
anyone have any papers written or studies done on the use of Eosin to stain
small biopsies or the use of eosin on the tissue processor in the last
alcohol??  I have read snippets on eosin having an effect on FISH testing??
Does anyone know of this to be true and have any papers or studies to refer
back to or any documentation showing this result??
One more thing does anyone use Hematoxylin to mark small tissue biopsies
and/or any thoughts on its use to do so?? My thoughts are that because of
it's precipitation qualities it could gunk up the tissue processor and leave
residue precipitate at embedding.. agree? Disagree?

Thanks for any responses.

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Re: [Histonet] Eosin staining for small biopsies

[Cynthia Robinson]

We use safranin (used in Microbiology as a counterstain)  on our small 
biopsies. We apply a small drop during grossing. It does not affect staining of 
H&E, IHC or ISH. We like this because it is an intense red that doesn't leach 
out in the alcohols of processor.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


>>> "Freeman, Carol"  6/20/2012 8:46 AM >>>


Good Morning all, Happy Wednesday!

I have a question and I am not finding much on my google search...First
does anyone have any papers written or studies done on the use of Eosin
to stain small biopsies or the use of eosin on the tissue processor in
the last alcohol??  I have read snippets on eosin having an effect on
FISH testing?? Does anyone know of this to be true and have any papers
or studies to refer back to or any documentation showing this result??
One more thing does anyone use Hematoxylin to mark small tissue biopsies
and/or any thoughts on its use to do so?? My thoughts are that because
of it's precipitation qualities it could gunk up the tissue processor
and leave residue precipitate at embedding.. agree? Disagree?

Thanks for any responses.

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RE: [Histonet] eosin bleeding on frozen sections

[Laurie Colbert]

Try dehydrating longer.  If there is water left on the slide, the eosin
will bleed.
Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christi
Cosby
Sent: Thursday, April 21, 2011 9:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] eosin bleeding on frozen sections

Hi,

In our lab we are having trouble with the eosin bleeding off the section
after coverslipping.  We are performing a rapid H&E stain on frozen
sections.  Any advice would be greatly appreciated!
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RE: [Histonet] eosin bleeding on frozen sections

[sgoebel]

This happened to me a couple weeks ago.  You got water on the slide
after the eosin staining.  Just run it back through alcohols, and rinse
for about five minutes.  Change out the alcohols, and re-stain.

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christi
Cosby
Sent: Thursday, April 21, 2011 11:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] eosin bleeding on frozen sections

Hi,

In our lab we are having trouble with the eosin bleeding off the section
after coverslipping.  We are performing a rapid H&E stain on frozen
sections.  Any advice would be greatly appreciated!
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Re: [Histonet] eosin in processor

[Patrick Laurie]

We don't, Leica recommends that you don't.  We have our grossing group
squirt a 0.1% Basic Fuchsin on the small translucent or white specimens.  It
leaves it light pink like the eosin.

On Fri, Oct 22, 2010 at 11:09 AM, Rathborne, Toni <
trathbo...@somerset-healthcare.com> wrote:

> Does anyone use eosin in their Peloris?
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Shea's
> Sent: Friday, October 22, 2010 2:07 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] eosin in processor
>
>
> This is the best kept secretFor those who have never tried this, you
> don't know what you are missing in the ease of embedding and cutting.
>
> We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100%
> alcohols, leaving the last 100% pure (of course there is carry over during
> the week), then we dump the first and rotate the other 2 each week. The one
> that we now moved into position #2 will get 10cc eosin).
>
> We send our IHC & FISH  to a HUGE reference lab. I specifically asked about
> this interfering and the technical rep said that it is not a problem esp
> they see a lot of it and they know to avoid non specific perimeter staining
> (the Pathologists know how to read around this because many labs are using
> Eosin in the processors).
>
> J
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> exemption from disclosure under applicable law.  Unauthorized forwarding,
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-- 
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CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plau...@cellnetix.com
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RE: [Histonet] eosin in processor

[Rathborne, Toni]

Does anyone use eosin in their Peloris? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Shea's
Sent: Friday, October 22, 2010 2:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] eosin in processor


This is the best kept secretFor those who have never tried this, you don't 
know what you are missing in the ease of embedding and cutting.

We have been adding alcoholic eosin (about 10cc each) to the 1st & 2nd 100% 
alcohols, leaving the last 100% pure (of course there is carry over during the 
week), then we dump the first and rotate the other 2 each week. The one that we 
now moved into position #2 will get 10cc eosin). 

We send our IHC & FISH  to a HUGE reference lab. I specifically asked about 
this interfering and the technical rep said that it is not a problem esp they 
see a lot of it and they know to avoid non specific perimeter staining (the 
Pathologists know how to read around this because many labs are using Eosin in 
the processors).

J
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RE: [Histonet] eosin

[Cathy]

We add 1.0 mls of 10% aqueous Eosin to the first 100% Ethanol; we will then
have Eosin in all of the Ethanol containers after they have been rotated.
This removed the time consuming task of dotting each tissue at grossing and
allows for easy visualization at embedding and sectioning.  I have not had a
complaint about the eosin interfering with FISH from our referral lab.

 

Cathy Fitzpatrick

Anatomic Pathology Section Head

Kelowna General Hospital

 

 

  _  

From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histot...@imagesbyhopper.com
Sent: Friday, October 22, 2010 8:18 AM
To: Tench, Bill
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] eosin

 

It sounds like I am in the minority in pitting the eosin in the *first* 100%
alcohol! 

I agree with Bill, it really makes a HUGE difference in small, minute
tissues.  We did run into one issue though, we were using orange biopsy
cassettes and the red-orange tissue was difficult to spot in the orange
cassettes!  We have since switched to an aqua color and no more hiding
specimens!  ;o)

Michelle



On Oct 22, 2010, at 10:51 AM, "Tench, Bill"  wrote:

> I think the use in a processor becomes a local culture.  I think
> everyone in my community does it (someone spread the word), so now we
> all do.  I believe that we are all putting it in the last alcohol.  It
> really makes facing a block on minute pieces a whole lot easier.  We
> found marking the tissue directly tedious, and it didn't persist as well
> in the processing.
>  Because there is often so little visible material in our FNA cell
> blocks, we mark the histogel pellet with Davidson ink, and when the tech
> has just faced off the ink, he/she knows its time to start collecting
> sections.
>
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> bill.te...@pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604
>
>
> [None] made the following annotations
> -
> Confidential E-Mail: This e-mail is intended only for the person or entity
to which it is addressed, and may contain information that is privileged,
confidential, or otherwise protected from disclosure. Dissemination,
distribution, or copying of this e-mail or the information herein by anyone
other than the intended recipient is prohibited. If you have received this
e-mail in error, please notify the sender by reply e-mail, and destroy the
original message and all copies.
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>
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RE: [Histonet] eosin

[Blazek, Linda]

We put eosin in the first 100% alcohol.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Friday, October 22, 2010 11:18 AM
To: Tench, Bill
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] eosin

It sounds like I am in the minority in pitting the eosin in the *first* 100% 
alcohol!  

I agree with Bill, it really makes a HUGE difference in small, minute tissues.  
We did run into one issue though, we were using orange biopsy cassettes and the 
red-orange tissue was difficult to spot in the orange cassettes!  We have since 
switched to an aqua color and no more hiding specimens!  ;o)

Michelle



On Oct 22, 2010, at 10:51 AM, "Tench, Bill"  wrote:

> I think the use in a processor becomes a local culture.  I think
> everyone in my community does it (someone spread the word), so now we
> all do.  I believe that we are all putting it in the last alcohol.  It
> really makes facing a block on minute pieces a whole lot easier.  We
> found marking the tissue directly tedious, and it didn't persist as well
> in the processing.
>  Because there is often so little visible material in our FNA cell
> blocks, we mark the histogel pellet with Davidson ink, and when the tech
> has just faced off the ink, he/she knows its time to start collecting
> sections.
> 
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> bill.te...@pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604
> 
> 
> [None] made the following annotations
> -
> Confidential E-Mail: This e-mail is intended only for the person or entity to 
> which it is addressed, and may contain information that is privileged, 
> confidential, or otherwise protected from disclosure. Dissemination, 
> distribution, or copying of this e-mail or the information herein by anyone 
> other than the intended recipient is prohibited. If you have received this 
> e-mail in error, please notify the sender by reply e-mail, and destroy the 
> original message and all copies. 
> -
> 
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Re: [Histonet] eosin

[histot...@imagesbyhopper.com]

It sounds like I am in the minority in pitting the eosin in the *first* 100% 
alcohol!  

I agree with Bill, it really makes a HUGE difference in small, minute tissues.  
We did run into one issue though, we were using orange biopsy cassettes and the 
red-orange tissue was difficult to spot in the orange cassettes!  We have since 
switched to an aqua color and no more hiding specimens!  ;o)

Michelle



On Oct 22, 2010, at 10:51 AM, "Tench, Bill"  wrote:

> I think the use in a processor becomes a local culture.  I think
> everyone in my community does it (someone spread the word), so now we
> all do.  I believe that we are all putting it in the last alcohol.  It
> really makes facing a block on minute pieces a whole lot easier.  We
> found marking the tissue directly tedious, and it didn't persist as well
> in the processing.
>  Because there is often so little visible material in our FNA cell
> blocks, we mark the histogel pellet with Davidson ink, and when the tech
> has just faced off the ink, he/she knows its time to start collecting
> sections.
> 
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> bill.te...@pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604
> 
> 
> [None] made the following annotations
> -
> Confidential E-Mail: This e-mail is intended only for the person or entity to 
> which it is addressed, and may contain information that is privileged, 
> confidential, or otherwise protected from disclosure. Dissemination, 
> distribution, or copying of this e-mail or the information herein by anyone 
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Re: [Histonet] Eosin to dye small Biopsies

[DKBoyd]

We use eosin at the grossing bench on endoscopic specimens and hematoxylin 
on prostate/liver biopsies.  The pathologist drops a drop of stain on each 
specimen. 

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkb...@chs.net







"Scott, Allison D"  
Sent by: histonet-boun...@lists.utsouthwestern.edu
10/21/2010 04:37 PM

To

cc

Subject
[Histonet] Eosin to dye small Biopsies






Hello to all in histoland.  Are any of you using eosin on the processor
to dye your small bx's?  If so, are you putting it in the 100% alcohol
to do so?  Any help in this matter will be greatly appreciated.


Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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Re: [Histonet] Eosin to dye small Biopsies

[godsgal...@aol.com]

We use cobalt blue as it does not interfere with FISH 

Sent from my Verizon Wireless Phone

- Reply message -
From: susan.wal...@hcahealthcare.com
Date: Fri, Oct 22, 2010 3:28 am
Subject: [Histonet] Eosin to dye small Biopsies
To: , 
Cc: 


We use about the same amount but in our last 100% Alc.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Thursday, October 21, 2010 4:40 PM
To: Scott, Allison D
Cc: 
Subject: Re: [Histonet] Eosin to dye small Biopsies

We use about 40ml of eosin in the first 100% alcohol in both of our "large 
specimen" & "small biopsy" machines.

Michelle



On Oct 21, 2010, at 4:33 PM, "Scott, Allison D"  
wrote:

> Hello to all in histoland.  Are any of you using eosin on the processor
> to dye your small bx's?  If so, are you putting it in the 100% alcohol
> to do so?  Any help in this matter will be greatly appreciated.
> 
> 
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> Houston, Texas 77026
> CONFIDENTIALITY NOTICE:
> If you have received this e-mail in error, please immediately notify the
> sender by return e-mail and delete this e-mail and any attachments from 
> your computer system.
> 
> To the extent the information in this e-mail and any attachments contain 
> protected health information as defined by the Health Insurance Portability 
> and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
> 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
> privileged.  This e-mail may also be confidential and/or privileged under 
> Texas law.  The e-mail is for the use of only the individual or entity named 
> above.  If you are not the intended recipient, or any authorized 
> representative of the intended recipient, you are hereby notified that any 
> review, dissemination or copying of this e-mail and its attachments is 
> strictly prohibited.
> 
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RE: [Histonet] Eosin to dye small Biopsies

[Susan.Walzer]

We use about the same amount but in our last 100% Alc.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Thursday, October 21, 2010 4:40 PM
To: Scott, Allison D
Cc: 
Subject: Re: [Histonet] Eosin to dye small Biopsies

We use about 40ml of eosin in the first 100% alcohol in both of our "large 
specimen" & "small biopsy" machines.

Michelle



On Oct 21, 2010, at 4:33 PM, "Scott, Allison D"  
wrote:

> Hello to all in histoland.  Are any of you using eosin on the processor
> to dye your small bx's?  If so, are you putting it in the 100% alcohol
> to do so?  Any help in this matter will be greatly appreciated.
> 
> 
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> Houston, Texas 77026
> CONFIDENTIALITY NOTICE:
> If you have received this e-mail in error, please immediately notify the
> sender by return e-mail and delete this e-mail and any attachments from 
> your computer system.
> 
> To the extent the information in this e-mail and any attachments contain 
> protected health information as defined by the Health Insurance Portability 
> and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
> 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
> privileged.  This e-mail may also be confidential and/or privileged under 
> Texas law.  The e-mail is for the use of only the individual or entity named 
> above.  If you are not the intended recipient, or any authorized 
> representative of the intended recipient, you are hereby notified that any 
> review, dissemination or copying of this e-mail and its attachments is 
> strictly prohibited.
> 
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RE: [Histonet] Eosin to dye small Biopsies

[Baez, Janet]

We put the eosin in the last 100% alcohol on the processor for all
specimens. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Thursday, October 21, 2010 1:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin to dye small Biopsies

Hello to all in histoland.  Are any of you using eosin on the processor
to dye your small bx's?  If so, are you putting it in the 100% alcohol
to do so?  Any help in this matter will be greatly appreciated.


Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain

protected health information as defined by the Health Insurance
Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160
and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential
and/or 
privileged.  This e-mail may also be confidential and/or privileged
under 
Texas law.  The e-mail is for the use of only the individual or entity
named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that
any 
review, dissemination or copying of this e-mail and its attachments is 
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Re: [Histonet] Eosin to dye small Biopsies

[histot...@imagesbyhopper.com]

We use about 40ml of eosin in the first 100% alcohol in both of our "large 
specimen" & "small biopsy" machines.

Michelle



On Oct 21, 2010, at 4:33 PM, "Scott, Allison D"  
wrote:

> Hello to all in histoland.  Are any of you using eosin on the processor
> to dye your small bx's?  If so, are you putting it in the 100% alcohol
> to do so?  Any help in this matter will be greatly appreciated.
> 
> 
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> Houston, Texas 77026
> CONFIDENTIALITY NOTICE:
> If you have received this e-mail in error, please immediately notify the
> sender by return e-mail and delete this e-mail and any attachments from 
> your computer system.
> 
> To the extent the information in this e-mail and any attachments contain 
> protected health information as defined by the Health Insurance Portability 
> and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
> 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
> privileged.  This e-mail may also be confidential and/or privileged under 
> Texas law.  The e-mail is for the use of only the individual or entity named 
> above.  If you are not the intended recipient, or any authorized 
> representative of the intended recipient, you are hereby notified that any 
> review, dissemination or copying of this e-mail and its attachments is 
> strictly prohibited.
> 
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Re: [Histonet] Eosin

[Massimo]

Yes. Especially when you watch into a test tube an 1% eosin solution and it is 
going to be diluted with distilled water. At that point it can be used for the 
staining of histological slices.

Best Regards,

Massimo




Da: Rene J Buesa 
A: histonet@lists.utsouthwestern.edu; sgoe...@xbiotech.com
Inviato: Lun 18 ottobre 2010, 17:51:43
Oggetto: Re: [Histonet] Eosin

Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if 
you 
observe a solution with transmitted light.
René J.

--- On Mon, 10/18/10, sgoe...@xbiotech.com  wrote:


From: sgoe...@xbiotech.com 
Subject: [Histonet] Eosin
To: histonet@lists.utsouthwestern.edu
Date: Monday, October 18, 2010, 10:53 AM



   Once  upon a time I heard that eosin fluoresces is this true? = ; What
   color  does it show up?  Could this be used as a sort of back gr= ound
   stain so you can tell exactly how many cells are in the field?
   Sarah Goebel, B.A., HT (ASCP)
   Histotechnician
   = = XBiotech USA Inc.
   8201 East Riversid= e Dr. Bldg 4 Suite 100
   Austin, Texas=   78744
   (512)386-2907
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Re: [Histonet] Eosin

[Rene J Buesa]

Yes, eosin fluoresces with a greenish-yellowish hoe that can even be seen if 
you observe a solution with transmitted light.
René J.

--- On Mon, 10/18/10, sgoe...@xbiotech.com  wrote:


From: sgoe...@xbiotech.com 
Subject: [Histonet] Eosin
To: histonet@lists.utsouthwestern.edu
Date: Monday, October 18, 2010, 10:53 AM



   Once  upon a time I heard that eosin fluoresces is this true? = ; What
   color  does it show up?  Could this be used as a sort of back gr= ound
   stain so you can tell exactly how many cells are in the field?
   Sarah Goebel, B.A., HT (ASCP)
   Histotechnician
   = = XBiotech USA Inc.
   8201 East Riversid= e Dr. Bldg 4 Suite 100
   Austin, Texas=   78744
   (512)386-2907
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RE: [Histonet] Eosin in Processor and iHC

[Monfils, Paul]

There shouldn't be any problem if you are doing immunoperoxidase staining.  If 
you are doing fluorescence, you will have to be sure you remove all traces of 
eosin from the tissues since eosin itself is fluorescent.

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Re: [Histonet] Eosin too pink

[Kim Tournear]

We add 100mL of 100% alcohol to 150mL of Eosin to tone it down each day. 

~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson,  AZ
~Don't let your life end before it begins~
OU Rocks





From: "Smith, Lesley" 
To: histonet@lists.utsouthwestern.edu
Sent: Tue, March 9, 2010 7:51:46 AM
Subject: [Histonet] Eosin too pink

Our Pathologists have, over the last few weeks, complained that the eosin is 
too pink. We have not altered our procedures or reagents in any way. It seems 
to be particularly bad in endoscopic biopsies. Thanks

Lesley Smith
Senior Biomedical Scientist Cellular Pathology
The Yorkshire Clinic
Bradford Road
Bingley
BD16 1TW

Switchboard: +44 1274 550600 ext 3348
Direct Dial: +44 1274 550800
http: www.ramsayhealth.co.uk  


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RE: [Histonet] Eosin leaching out of sections

[Rathborne, Toni]

Where do you get the beads from?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Joyce
Cline
Sent: Wednesday, February 03, 2010 2:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin leaching out of sections 


Humidity has played a large part in our slides leaching Eosin. We have a 
dehumidifier in our staining room and we also use H2Blue Beads in our Xylene 
substitute. The beads absorb water from the substitute and help prevent 
leaching of the stain.

The beads are ordered from Amercian Mastertech.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott
Hendricksen
Sent: 03 February 2010 04:16
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections

Hi again,

Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an H&E stained slide?

Thanks,

Scott Hendricksen HT(ASCP)
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RE: [Histonet] Eosin leaching out of sections

[Joyce Cline]

Humidity has played a large part in our slides leaching Eosin. We have a 
dehumidifier in our staining room and we also use H2Blue Beads in our Xylene 
substitute. The beads absorb water from the substitute and help prevent 
leaching of the stain.

The beads are ordered from Amercian Mastertech.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott
Hendricksen
Sent: 03 February 2010 04:16
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections

Hi again,

Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an H&E stained slide?

Thanks,

Scott Hendricksen HT(ASCP)
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RE: [Histonet] Eosin leaching out of sections

[Bonner, Janet]

Your xylene and/or alcohols may need changing.
 
Janet 



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Scott Hendricksen
Sent: Wed 2/3/2010 9:02 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections 



> Hi again,
>
> Does anyone have an issue with eosin leaching out of sections a few days 
> after they have been coverslipped on an H&E stained slide?
>
> Thanks,
>
> Scott Hendricksen HT(ASCP)


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Re: [Histonet] Eosin evaporation.

[Rene J Buesa]

Before going home, cover the container
René J.

--- On Fri, 1/15/10, Cheri Miller  wrote:


From: Cheri Miller 
Subject: [Histonet] Eosin evaporation.
To: "histonet" , 
"histonet-boun...@lists..utsouthwestern.edu" 

Date: Friday, January 15, 2010, 10:05 AM


I recently purchased a leica refurbished autostainer, Before we hand stained 
our by hand. Now I notice the eosin is getting very low very fast, we have to 
replenish almost everyday. Before a bottle of Richard Allen Eosin would last 
the week or at least six days. Any idea's?

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148


PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you 
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RE: [Histonet] Eosin evaporation.

[Rathborne, Toni]

Are you keeping the lid to the stainer closed at all times, and are you 
covering each reagent container overnight? It should have come with a lid for 
each container.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Cheri
Miller
Sent: Friday, January 15, 2010 10:06 AM
To: histonet; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Eosin evaporation.


I recently purchased a leica refurbished autostainer, Before we hand stained 
our by hand. Now I notice the eosin is getting very low very fast, we have to 
replenish almost everyday. Before a bottle of Richard Allen Eosin would last 
the week or at least six days. Any idea's?

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148


PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you 
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promptly delete this message and notify the sender of the delivery error
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Re: [Histonet] Eosin evaporation.

[Paula Pierce]

During the winter months the air is drier, especially with central heat. 

My tap water is sometimes as if it has been refrigerated too.

Paula





From: Sheila Haas 
To: Cheri Miller ; "Sherwood, Margaret" 
; "Breeden, Sara" ; "Mahoney, 
Janice A" ; histonet 
; 
"histonet-boun...@lists.utsouthwestern.edu" 

Sent: Fri, January 15, 2010 10:22:32 AM
Subject: Re: [Histonet] RE: Eosin evaporation.

Cheri,
How close on your stain line is your eosin in relation to the oven?
It's possible if it nearby, the oven is evaporating your eosin more
rapidly than expected. Just a thought.
 
Sheila Haas
Laboratory Supervisor
Micro Path Laboratories
 





From: Cheri Miller 
To: "Sherwood, Margaret" ; "Breeden, Sara" 
; "Mahoney, Janice A" ; 
histonet ; 
"histonet-boun...@lists.utsouthwestern.edu" 

Sent: Fri, January 15, 2010 11:14:30 AM
Subject: RE: [Histonet] RE: Eosin evaporation.

We do this as well, that is why I am stumped.

Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148

-Original Message-
From: Sherwood, Margaret [mailto:msherw...@partners.org]
Sent: Friday, January 15, 2010 9:53 AM
To: Breeden, Sara; Mahoney,Janice A; Cheri Miller; histonet; 
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.

I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also try to limit the sections to the bottom half or third of the sldie.



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RE: [Histonet] Eosin Bleeding

[Monfils, Paul]

Can't say for sure but eosin is very sensitive to pH, and will rinse out 
rapidly in any solution that is basic, even slightly so, like tap water.  Nair 
contains calcium hydroxide, which is a stong base. If that compound were not 
thoroughly rinsed out of the tissue before processing, I wouldn't be surprised 
if it interfered with eosin binding.

> --
> From: histonet-boun...@lists.utsouthwestern.edu on behalf of Goins, 
> Tresa
> Sent: Tuesday, August 4, 2009 5:11 PM
> To:   histonet@lists.utsouthwestern.edu
> Subject:  [Histonet] Eosin Bleeding 
> 
> We very occasionally have a single slide out of a group of 100 or more where 
> the eosin will bleed after coverslipping.  Does anyone know if this is 
> associated with a particular tissue type or treatment with Nair to soften 
> keratin?
> 
> Thanks,
> 
> Tresa
> Veterinary Diagnostic Lab
> Bozeman, MT
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RE: [Histonet] Eosin in Alcohol

[Maria Katleba]

I wonder (with the economy so bad) that there are CHEAP eosin products out 
there. Could that be it?.. using of cheap 'knock off' eosins..
MK

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Wednesday, July 15, 2009 3:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin in Alcohol

News to me  We have used this for many years and have never had a problem 
with IHC ourselves or heard of anyone else having issues.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Harrison,
Sandra C.
Sent: Tuesday, July 14, 2009 5:15 PM
To: Jennifer Johnson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin in Alcohol


"polycyclic aromatic flourescent compounds that in high concentrations"
  I wouldn't think the 3 mls of eosin dropped in the last 95%
alcohol could be considered "high concentration" but that's what keeps
Histonet entertaining; I learn something new every day.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Johnson
Sent: Tuesday, July 14, 2009 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin in Alcohol


A couple of weeks ago I posted the message below on the histonet and all
of you responded that it shouldn't matter so I have finally gotten a
reply from the company we send our prostate biopsies off to and below is
their response.  So now you know the rest of the story!



We have used Eosin in the last 95% alcohol on the tissue processor for
several years. I usually add approximately 5 ml to the full jug. It is a
great tool to use for embedding. However, we received a letter from the
lab that we send our prostate biopsies to saying that it was undesirable
because it interfered with their immuno staining. They sent us some
cobalt blue to use in the place of eosin along with mixing instructions
and the whole batch of tissues came out such a dark blue. There is no
delineations in the color of the blue and I found it to be useless for
helping to embed. I would rather do without anything than use cobalt
blue. I guess the point of my rambling is, Eosin is a wonderful tool to
use unless you are doing immunos on prostate biopsies.

Thanks,

Jennifer Johnson, HTL (ASCP)


Their reply:  "The problem is that eosin belongs to a family of
polycyclic aromatic flourescent compounds that in high concentrations
binds to and saturates all tissue  components.  When immunoflourescence
is performed on such tissue- as in the prostate px+ test- the diffuse
background autoflourescence signal from prior treatment with these
compounds can interfere with, and even totally overwhelm, the signal of
the flourescent-labeled antibodies used to localize biomarkers in the
tissue."





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RE: [Histonet] Eosin in Alcohol

[Tom McNemar]

News to me  We have used this for many years and have never had a problem 
with IHC ourselves or heard of anyone else having issues.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Harrison,
Sandra C.
Sent: Tuesday, July 14, 2009 5:15 PM
To: Jennifer Johnson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosin in Alcohol


"polycyclic aromatic flourescent compounds that in high concentrations"
  I wouldn't think the 3 mls of eosin dropped in the last 95%
alcohol could be considered "high concentration" but that's what keeps
Histonet entertaining; I learn something new every day.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Johnson
Sent: Tuesday, July 14, 2009 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin in Alcohol


A couple of weeks ago I posted the message below on the histonet and all
of you responded that it shouldn't matter so I have finally gotten a
reply from the company we send our prostate biopsies off to and below is
their response.  So now you know the rest of the story!

 

We have used Eosin in the last 95% alcohol on the tissue processor for
several years. I usually add approximately 5 ml to the full jug. It is a
great tool to use for embedding. However, we received a letter from the
lab that we send our prostate biopsies to saying that it was undesirable
because it interfered with their immuno staining. They sent us some
cobalt blue to use in the place of eosin along with mixing instructions
and the whole batch of tissues came out such a dark blue. There is no
delineations in the color of the blue and I found it to be useless for
helping to embed. I would rather do without anything than use cobalt
blue. I guess the point of my rambling is, Eosin is a wonderful tool to
use unless you are doing immunos on prostate biopsies. 
 
Thanks,
 
Jennifer Johnson, HTL (ASCP) 


Their reply:  "The problem is that eosin belongs to a family of
polycyclic aromatic flourescent compounds that in high concentrations
binds to and saturates all tissue  components.  When immunoflourescence
is performed on such tissue- as in the prostate px+ test- the diffuse
background autoflourescence signal from prior treatment with these
compounds can interfere with, and even totally overwhelm, the signal of
the flourescent-labeled antibodies used to localize biomarkers in the
tissue."

 

 

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RE: [Histonet] Eosin in Alcohol

[Harrison, Sandra C.]

"polycyclic aromatic flourescent compounds that in high concentrations"
  I wouldn't think the 3 mls of eosin dropped in the last 95%
alcohol could be considered "high concentration" but that's what keeps
Histonet entertaining; I learn something new every day.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Johnson
Sent: Tuesday, July 14, 2009 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin in Alcohol


A couple of weeks ago I posted the message below on the histonet and all
of you responded that it shouldn't matter so I have finally gotten a
reply from the company we send our prostate biopsies off to and below is
their response.  So now you know the rest of the story!

 

We have used Eosin in the last 95% alcohol on the tissue processor for
several years. I usually add approximately 5 ml to the full jug. It is a
great tool to use for embedding. However, we received a letter from the
lab that we send our prostate biopsies to saying that it was undesirable
because it interfered with their immuno staining. They sent us some
cobalt blue to use in the place of eosin along with mixing instructions
and the whole batch of tissues came out such a dark blue. There is no
delineations in the color of the blue and I found it to be useless for
helping to embed. I would rather do without anything than use cobalt
blue. I guess the point of my rambling is, Eosin is a wonderful tool to
use unless you are doing immunos on prostate biopsies. 
 
Thanks,
 
Jennifer Johnson, HTL (ASCP) 


Their reply:  "The problem is that eosin belongs to a family of
polycyclic aromatic flourescent compounds that in high concentrations
binds to and saturates all tissue  components.  When immunoflourescence
is performed on such tissue- as in the prostate px+ test- the diffuse
background autoflourescence signal from prior treatment with these
compounds can interfere with, and even totally overwhelm, the signal of
the flourescent-labeled antibodies used to localize biomarkers in the
tissue."

 

 

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Lauren found her dream laptop. Find the PC that's right for you.
http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290__
_
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Re: [Histonet] eosin in processing alcohols

[Anne van Binsbergen]

we put phloxine in the formalin - we find it a better option than eosin

2009/7/1 Beth Couch 

> Hi Histonetters - do any of you use easin in the processing alcohols in
> order to better visualize small bipsies and if so how much eosin to alcohol
> do you use and in which alcohol?
>  Thank you in advance for your help.
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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Re: [Histonet] eosin pH

[Rene J Buesa]

The eosin should be acidic and you get to that point adding 1% aq. sol. of 
acetic acid to you prepared solution, at a rate of 1 mL acetic/100 mL of eosin 
solution.
René J.

--- On Mon, 10/20/08, Hana Peter <[EMAIL PROTECTED]> wrote:

From: Hana Peter <[EMAIL PROTECTED]>
Subject: [Histonet] eosin pH
To: histonet@lists.utsouthwestern.edu
Date: Monday, October 20, 2008, 4:29 AM

Hi!
Can someone tell me the right pH for eosin y 1% aqueous solution?
The one I made from powder has pH 6.3. Is this OK? The commercial one we 
usually buy is a bit lower (5.7).
Thank you in advance!
Hana Peter

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