Re: [ccp4bb] off-topic: tag removal

2011-02-23 Thread Dima Klenchin
Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, et al. 2009 Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag. PLoS ONE 4(12): e8119. doi:10.1371/journal.pone.0008119 Unless the protein in question happen to be Leu as a C-terminal residue, this

[ccp4bb] Postdoc and PhD student positions, Biozentrum Basel

2011-02-23 Thread Timm Maier
Postdoc and PhD student positions, Biozentrum Basel, Maier Lab Our new lab at the Biozentrum is part of the core program "Structural Biology & Biophysics", which gathers excellence in molecular and nanoscience research including structural studies by electron microscopy, biological NMR and X-ray c

Re: [ccp4bb] scala : cornercorrect file

2011-02-23 Thread Phil Evans
I'm not sure that I would recommend this Phil On 23 Feb 2011, at 17:03, Bryan Lepore wrote: > if anyone knows of a cornercorrect file I might be able to use in > scala, perhaps for an adsc q[any], please let me know off-list if you > don't mind. > > -Bryan

[ccp4bb] scala : cornercorrect file

2011-02-23 Thread Bryan Lepore
if anyone knows of a cornercorrect file I might be able to use in scala, perhaps for an adsc q[any], please let me know off-list if you don't mind. -Bryan

[ccp4bb] PISA ligand handling

2011-02-23 Thread Katherine Sippel
Hi all, I have sort of an oddball question regarding how PISA reads the ligand file into the program for calculations. Does the element symbol of the PDB file take precedence over the atom name or residue name? Specifically, if a ligand contains either atom names or residue names that are inconsi

Re: [ccp4bb] off-topic: tag removal

2011-02-23 Thread Eric Larson
Hi Phil, Must you tag your protein? Have you tried to express it without a tag? Perhaps you can purify the un-tagged protein with a series of chromatographic (or other) steps - particularly if it expresses well. Eric Eric T. Larson, PhD Biomolecular Structur

Re: [ccp4bb] off-topic: tag removal

2011-02-23 Thread Dima Klenchin
I have a question concerning removal of a his-tag sequence. We have crystallized a protein with an important feature at the C-terminal part of the protein. Unfortunately, we cannot express it with a N-terminal his-tag, only with a C-terminal his-tag. Therefore we are looking for a protease whi

Re: [ccp4bb] off-topic: tag removal

2011-02-23 Thread Bosch, Juergen
Do you have the crystal structure ? Then look for a surface loop/exposed area and insert the His6-tag there. Jürgen On Feb 23, 2011, at 4:17 AM, Philipp Ellinger wrote: Dear all, I have a question concerning removal of a his-tag sequence. We have crystallized a protein with an important feature

Re: [ccp4bb] off-topic: tag removal

2011-02-23 Thread Srivastava, Dhiraj (MU-Student)
See the following reference- Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, et al. 2009 Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag. PLoS ONE 4(12): e8119. doi:10.1371/journal.pone.0008119 From: CCP4 bulle

[ccp4bb] off-topic: tag removal

2011-02-23 Thread Philipp Ellinger
Dear all, I have a question concerning removal of a his-tag sequence. We have crystallized a protein with an important feature at the C-terminal part of the protein. Unfortunately, we cannot express it with a N-terminal his-tag, only with a C-terminal his-tag. Therefore we are looking for a prote

Re: [ccp4bb] post-doc and technician position at KULeuven, Belgium - structural biology of ion channel homologs

2011-02-23 Thread Chris Ulens
A position is available for a post-doctoral researcher and technician at the Laboratory for Structural Neurobiology at the KULeuven, Belgium. http://www.xtal.be Our research focus is on ion channel homologs from different classes. Our strategy to identify targets for structural studies relie