hi sudhir,
i think in second snapshot you can fit MPD as its structure is
seems similar. .
On Fri, Feb 10, 2012 at 11:36 AM, Sudhir Kumar wrote:
> Dear all,
> I have a 2 A structure of an enzyme which show 12 molecules per
> asymmetric unit. While placing waters i found out some blobs wh
Dear All,
I wrote a story for the catalysis of Orotidine 5'-Monophosphate
Decarboxylase.
http://www.jinkai.org/Enzymology.html
I hope you will like it.
Regards,
Kevin
assuming your protein has a Y or a W...before buying a dye or trying an elisa,
it would be cheapest and easiest to check for Trp or Tyr fluorescence changes
upon adding the inhibitor. If the structure or a structural homolog is known of
one of the proteins and you know the interface, a simple PC
Dear colleagues,
I appreciate your help in interpreting the pKa shift. Based on all the
suggestions, I have several new leads to be able to come up with a
tentative mechanism. I am summarizing the discussion below –
The pKa of a catalytically critical aspartic acid has increased to 6.44. It
is hy
You have been all wonderfully helpful.
The landscape is crystal-clear now.
Thanks to everybody.
Giorgio
Hi Joe,
Non-natural amino acids and links etc. remain a moving target. Almost
every year, refinement programs change the way these things are
specified. Here is how I would do it:
1) search the protein data bank to see if your non-natural amino acid is
already present somewhere.
If yes, use the
Hi Giorgo,
Just to say that we routinely, and often, do these sorts of
experiments on ID29 and ID23-1 at the ESRF using an energy of 20 keV
(~0.62 Angstrom wavelength) and detector distances that allow
collection of data to a resolution of better than 0.7A. Mini-kappa
goniometers also allow
