Re: [ccp4bb] Substituting zero vs. Fc for unobserved reflections

Thank you for your responses. Actually, the data are very complete: 99.6% total and 99.2% in the high resolution shell. Only 2% of data were used for cross validation because there were a large number of reflections. I had calculated omit maps. At first when I discovered the presence of the lower

[ccp4bb] postdoctoral position in structural neurobiology

A postdoctoral position at Yale University School of Medicine is available immediately for highly motivated, creative individuals with a strong interest in the structure, function, and pharmacology of signaling proteins implicated in neurological and neuropsychiatric diseases. We are presently focu

Re: [ccp4bb] Emerald Plugmaker experiences?

its ability to fine slice a known crystallization space is remarkable.We personally do not have this machine.I only have used it when it came for a demo. In that time the use of some known membrane protein crystallization space without even much careful experimentation resulted in hits. We have a

[ccp4bb] Emerald Plugmaker experiences?

Hi, I am looking for feedback from those who have used the Emerald Plugmaker instrument, especially if in your experience the translation of initial crystallization hits to diffracting crystals was as straightforward as claimed by Emerald. Any other thoughts and comments are also welcome. T

Re: [ccp4bb] P21, twinned date & NCS refinement

Thanks Pavel for the tip, will the ncs.type=torsion work with 1.7.3-928 or do I have to update ? We have >90% complete data to 2.3Å and 40% completeness to 2.1 Å. Jürgen On Mar 28, 2012, at 2:15 AM, Pavel Afonine wrote: Hi Jürgen, in phenix.refine I would do: phenix.refine [PDB] [mtz] twin

[ccp4bb] Postdoctor​al position in Grenoble (France)

POSTDOCTORAL POSITION - INSTITUT DE BIOLOGIE STRUCTURALE, GRENOBLE, FRANCE POSITION: The Structural Protein Dynamics group at the Institut de Biologie Structurale in Grenoble is looking for a highly motivated researcher to join the team as a postdoc to work for 30 months on a project funded by

[ccp4bb] dear all

Dear all  yes my paalet is large enough and no my protein is not soluble ,and  I am using 37degrees before and after induction with IPTG,and the vector that I am using is pRSETA 3.2kd Best Regards Rana From: Kelly Daughtry To: rana ibd Cc: CCP4BB@jiscmail.a

[ccp4bb] Research Associate position at Imperial College London - on behalf of Prof Fairweather

Dear all, Please find below an ad for a post-doctoral researcher vacancy at Imperial, posted hereon behalf of Prof Fairweather. Please reply directly to him or the email refered in the ad. Best regards Paula *Imperial College London, Division of Cell & Molecular Biology, Faculty of Natural Sc

Re: [ccp4bb] Notebook and stereo

Dear All, I have successfully setup Toshiba Qosmio F7500 -3D gaming laptop for molecular visualization purposes. This 15" notebook computer can support 3D stereo in Coot and Pymol in glasses-free 3D mode. It works for 2D and 3D Windows7 applications in parallel. Details on the settings can be

[ccp4bb] Postdoc/PhD positions available in Bristol

Dear All, please see below for links detailing two positions currently available here in Bristol, 1x 2 yr PDRA position in natural product enzymology, and 1x 3 yr PhD studentship in the structural biology of strep virulence. The former would ideally suit a postdoctoral level scientist wit