Hi Giulliana,
You can check our general protocol for TEV digestion
(http://www.accelagen.com/TurboTEV-protocol.htm). Once you mixed TEV with
protein solution, let it sit for digestion. No shaking or rocking. We found
shaking or rocking sometimes reduce the efficiency.
Some constructs jus
Hi, as I understand your question, you're asking whether the EM density
values in a cryo-EM map ('MRC' format) would be expected to be comparable
with the corresponding electron density values in a CCP4 XRD map obtained
by FT of the map coefficients from an MTZ X-ray reflexion file at the same
nomi
Hey Giulliana,
does your protein buffer contain high imidazole concentrations ( > 150 mM)? If
so you should try to exchange the buffer before cleavage, since the TEV
protease tends to precipitate at higher imidazole concentrations.
Schara
> Am 02.05.2015 um 19:56 schrieb Giulliana Rangel :
>
Hi,
I have used tev protease for tag cleavage during dialysis. In my case
always tev got precipitated not my protein. And cleavage was always
complete. I havE checked on the sds - page as wel.
On 2 May 2015 23:27, "Giulliana Rangel" wrote:
> Dear all,
>
> I'd like some help about my protein cause
Hi,
On Sun, May 3, 2015 at 4:15 AM, Smith Liu wrote:
> If for both a mtz density and mrc map I set the contour level as 0.15,
> does the 015 has the comparable significance for the mtz density and mrc
> map?
>
may be. Depends how your map (3D grid function in "mrc" file) and Fourier
map coeffic
If for both a mtz density and mrc map I set the contour level as 0.15, does the
015 has the comparable significance for the mtz density and mrc map?
Smith
