Hmmm…. One wonders how difficult it is to ignore the ‘R’ which is enough in
links to non-symmetry-related atoms.
if (line(1:5).eq.'LINKR') line= 'LINK '//line (6:59)
PS: does anyone else find that the ‘Major validation issues’ web part on PDBe
deposition does not update correctly today?
Hi Firdous,,
As you mentioned they are many things to change and try.
before changing vector it is more efficient in my opinion to change
expression conditions (temperature, amount of IPTG, length of culture),
culture medium (regular ones against M9 or auto induction media) and
expression strains
Hey Firdous
Yes, too much information can be confusing. I have expressed and purified
over dozen of different proteins. I usually found in literature the
protocol for the expression and purification of another homologous protein.
That is the best way to start in my opinion. Some folks may want to
Hello everyone.
I am struggling with the solubility or expression of my proteins in BL21DE3
E.coli expression cells. In one of my construct I have a Sumo-His fusion at
the n-terminal of my protein. After induction what I see is the expression
of the Sumo tag only. My sequencing results are fine an
Yes - I agree about the irritation, but the LINKR record is actally more
informative than the LINK
LINKR can point to a named LINK which involves angles as well as a
distance..
I think LINKR defines a needed coordinate feature but how to get the wwwPDB
to accept it???
E
On 9 December 2017 at
Dear Developers,
the TER issue in REFMAC seems to be fixed, but is really necessary that
REFMAC places LINKR
records instead of LINK records in the PDB header?
The PDB does not recognize those, and although it's only a minor nuisance to
fix them in an editor,
one tends to forget this bet
On 09/12/2017 15:56, chemocev marker wrote:
I am wondering is there clear way to show these forces like H-bond. I tried
KING and can see the VDW radii but not the visual representation. Does coot has any option for this?
Coot uses the VdW radii in the Refmac monomer library.
Maybe Measures -
Hi
Thanks for your good help and I am wondering is there clear way to show
these forces like H-bond. I tried KING and can see the VDW radii but not
the visual representation. Does coot has any option for this.
best
Jiri
On Fri, Dec 8, 2017 at 8:57 AM, HERMAN VAN TILBEURGH <
herman.van-tilbeu...@u-
