Re: [ccp4bb] Crystal structure of an unknown protein

2017-12-16 Thread Petr Leiman
To keep this thread on topic - there is no Blue Stone on the Galveston island. Lots of white sand beaches, lots of black birds, lots of new BMW cars in the port (they unload the ship here), a few oil rigs, an occasional cruise ship, but no Blue Stone… Petr -

Re: [ccp4bb] Crystal structure of an unknown protein

2017-12-16 Thread Anastassis Perrakis
I wonder why you assume know there are "about 20 point mutation sites” if "this protein is an unknown protein”. It looks like you are comparing the sequence of a protein you do not know what it is to the sequence of a protein you dont really know what it is (1). I would consider it more likely,

Re: [ccp4bb] Crystal structure of an unknown protein

2017-12-16 Thread Ivan Shabalin
Hi Jiyong, Try running the FITMUNK server in the sequencing mode: http://witold.med.virginia.edu/fitmunk/server/ More information on using FITMUNK to identify your protein: https://www.ncbi.nlm.nih.gov/pubmed/26894674 And here: https://www.ncbi.nlm.nih.gov/pubmed/26660914 Ivan With best

[ccp4bb] Electrostatic Potential: Poisson-Boltzmann criteria

2017-12-16 Thread chemocev marker
Hi I am just calculating the Electrostatic Potential, and I wanted to know your opinion which force field is better.What is criteria to choose and not to choose the pKA... best Jiri