Dear Gloria,
probably even when you make the protein synthetically there will be
proteases in your solution, because many "air allergens" are proteases
(The major HDM allergens (i.e., allergens recognized by the majority of
HDM allergic subjects) are present in high amounts in the fecal pellet
Another option would be to use a protease immobilized in some resin (for
instance GST-tagged TEV protease bound to GSH-Agarose resin, in column or
in batch), so that no protease would be free to co-purify with your protein
or peptide.
HTH,
Javier
On Thu, Sep 20, 2018 at 5:35 PM, Patrick Loll wrot
1) Use a different protease, e.g. SUMO protease, which is sufficiently specific
that it’s unlikely to cause any problems even if a little bit is carried along.
See, for example (shameless plug #1): DOI: 10.1016/j.pep.2006.12.006
2) Use intein cleavage. NEB sells a vector that lets you fuse an in
Hello, friends in crystallography,
A colleague just asked me this question. He is worried about trace
protease interfering with the receptors he is studying in cell-based
experiments using a 110 amino acid protein we made for him. He has
been unable to make the peptide synthetically. The company
Proprietary
Merck is currently recruiting for a Senior Scientist, Cryo-EM . This role is
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This role will support drug discov
We should also be aware that there is a formal IUPAC definition of hydrogen
bonds as well, although its not really optimized for protein
crystallography.
https://www.iupac.org/publications/pac/pdf/2011/pdf/8308x1637.pdf
Matthew Merski
Univ. of Warsaw
On Thu, Sep 20, 2018 at 5:28 PM Mark Wilson
Just to put a finer point on some of this, the Lennard-Jones (6-12)
potential is short-range, containing an attractive component arising from
London dispersion forces (quantum mechanical induced dipole-induced dipole
interactions) that decay as 1/r^6 and a repulsive component arising from
the Pauli
Hello again. I just want to add that hydrogen bonds by convention are
usually
considered to be a type of electrostatic interaction (please see the review
by
E. N. Baker in the International Tables of Crystallography Volume F, second
edition,
p. 721) and are generally grouped with other "short rang
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Stefano is correct that hydrophobic interactions are chiefly entropically
driven. Thank you for your
input, Stefano. I disagree with Matthew, however. It is true that van der
Waals forces are always
present and therefore form a small contribution to even hydrogen bonds.
However, since the major
Dear Matthew,
In my search for the validity of meta data, I went through several data
sets in SBGrid and proteindiffraction.org (IRRMC), especially those
where automatic processing did not succeed are gave different results
with different processing software. We reprocessed those data sets wit
Dear Colleagues,
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Matthew,
you can find several examples here:
https://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Quality_Control
чт, 20 сент. 2018 г. в 3:18, Whitley, Matthew J :
> Dear colleagues,
>
> For teaching purposes, I am looking for a small number (< 5) of
> macromolecular diffraction datasets
Hi Matthew,
I have some notes which indicate that SBgrid data sets 5, 62, 78, 117, 218
posed problems for "automatic" processing using generate_XDS.INP / XDS when I
saw them for the first time.
Some of these problems (mainly the conversion of header values to ORGX ORGY)
are taken care of in cu
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