[ccp4bb] Fwd: [ccp4bb] Studying hydrogen bonding network by neutron diffraction in sample with D2O

-- Forwarded message - From: Arpita Goswami Date: Sun, Sep 8, 2024, 03:11 Subject: Re: [ccp4bb] Studying hydrogen bonding network by neutron diffraction in sample with D2O To: John R Helliwell Dear Dr. John and others, Good day! Thank you all for the clarifications

Re: [ccp4bb] Studying hydrogen bonding network by neutron diffraction in sample with D2O

but far from the pKa this will not be noticeable. > > On Fri, 6 Sept 2024, 04:12 Arpita Goswami, wrote: > >> Dear all, >> >> Good morning, Sorry for the off-topic question. >> >> There is a protein crystal sample in which water bonding networks by >>

[ccp4bb] Studying hydrogen bonding network by neutron diffraction in sample with D2O

Dear all, Good morning, Sorry for the off-topic question. There is a protein crystal sample in which water bonding networks by certain species to be studied by neutron diffraction, not exactly within the protein. But between the subunits of the protein. Now it is mandatory to use some D2O for the

Re: [ccp4bb] About Staraniso

Dear all, Good day. Thank you all for the very extensive discussions. Both on- and off-list discussions were very helpful. Thank you and a very happy Valentine's day to all.. Best regards, Arpita On Wed, Feb 14, 2024, 01:25 Kay Diederichs wrote: > Dear readers of CCP4BB, > > for various reas

[ccp4bb] About Staraniso

Dear all, Greetings to all! Apologies if the below query seems very naive! This is to query on the consensus to use Staraniso for pdb submission. We have solved a structure previously at 2.3 A resolution. The same data (after reindexing the diffraction images in autoPROC) and after reprocessing b

Re: [ccp4bb] Query on density fitting to phosphate

On Mon, Dec 18, 2023 at 7:01 AM Joel Tyndall wrote: > Have you tried sodium? > > > > *From:* CCP4 bulletin board *On Behalf Of *Arpita > Goswami > *Sent:* Sunday, December 17, 2023 11:47 PM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] Query on density fitting to

[ccp4bb] Query on density fitting to phosphate

Dear All, Hope you all are doing well. The density in the image (in link below) is fitted with PO4 ion, although the crystallization condition has both mono and dihydrogen phosphate which is not fitting without hydrogen. But the resolution is 2.5 A, so hydrogen may not be put in, or is there any

Re: [ccp4bb] Normal mode refinement

ng to high thermal factors. > Therefor I want to know is there any software/script available to execute > the normal mode of refinement. Thanks a lot in advance for your imperative > suggestions > > Appu > -- Arpita -- Arpita Goswami Senior Research Fellow Structural Biology

[ccp4bb] Pharmacia FPLC manual

Dear all, Sorry for off topic query. Does anyone have soft copy of Pharmacia FPLC user manual? If possible please mail that to me. Regards, apu

[ccp4bb] Cryoprotectant for jaffamine - summary

Hi all, Thanks for the reply. I have ultimately used 15% glycerol as cryo. It worked well. I referred to this paper McFerrin and Snell, Cryoprotectant quantification J. Appl. Cryst. (2002). 35, 538±545. Now setting up grid with different concentrations of jeffamine. Arpita Below is a summary

[ccp4bb] Apperance of ice ring in cryo after addition of protein crystal ( otherwise absent in only cryo)

, 0.1M HEPES (pH 7.5), 15% glycerol. Regards, Arpita. --- Arpita Goswami Junior Research Fellow Structural Biology Laboratory Centre for DNA Fingerprinting and Diagnostics (CDFD) Hyderabad