Hello,
Paul Craig, Herbert Bernstein, and Jeffrey Mills from the Rochester Institute
of Technology, along with Edward Pryor from Anatrace, are interested in the
development of educational kits for use in the laboratory component of
undergraduate lab courses. We have prepared a brief survey to
Hi, Shubhangi,
One possibility is that the his-tag could be non-accessible. Have you tried
moving to the tag to the other end of the protein (N vs C)? Another thing that
worked for me for a past project is using a small amount (1-2M) of urea for the
IMAC step and then dialyzing away the urea
