[ccp4bb] System for production of proteins with intrinsically disordered region (IDRs)

Hello! To those who have experience with the production of human proteins with intrinsically disordered region (IDRs), which expression system do you recommend? When bacteria doesn´t work... Mammalian cells, cell-free protein synthesis (CFPS) or any other system? Thank you! Best regards, Jul

[ccp4bb] RES: [ccp4bb] Large number of outliers in the dataset

; the multiplicity is Overall- 10.3 InnerShell - 9.5 OuterShell - 10.2; the diffraction spots are smeared. Bests regards, Juliana Juliana Ferreira de Oliveira Brazilian Laboratory of Biosciences - LNBio Brazilian Center for Research in Energy and Materials - CNPEM Campinas-SP, Brazil De: CCP4

[ccp4bb] Large number of outliers in the dataset

99.9 99.5 100.0 Multiplicity10.3 9.5 10.2 Average unit cell: 37.57 51.51 88.75 90.00 90.00 90.00 Space group: P212121 Average mosaicity: 1.90 Juliana Ferreira de Oliveira Brazilian La