[ccp4bb] Post-Doctoral opportunity in Pavia, Italy

2023-09-04 Thread Marco Lolicato
CV and a cover letter to: marco.lolic...@unipv.it Informal inquiries can be also addressed to the same e-mail. Kind Regards, Marco Lolicato -- Marco Lolicato, PhD Associate Professor of Molecular Biology Università di Pavia Dipartimento di Medicina Molecolare Palazzo Golgi / Spallanzani Via

[ccp4bb] 3rd International Workshop on Biological Membranes (19-22 August 2018, Helsinki)

2018-03-27 Thread Marco Lolicato
This might be of interest to someone: *** Dear All, We would like to remained you that *the early registration deadline (April 15, 2018)* for the 3rd International Workshop on Biological Membranes (19-22 August 2018, Helsinki) is approaching. Registration link: https://www.helsinki.fi/en/c

Re: [ccp4bb] OT: "Who's Afraid of Peer Review?"

2013-10-09 Thread Marco Lolicato
Hi scientists, this interesting topic brought back to my mind a similar discussion I had with a colleague of mine and now I want to share it with you guys. As Vale already pointed out, the peer-review process seems to be far from an ideal system: there are many papers in which one of the author i

Re: [ccp4bb] installation in sw or sw64?

2012-09-06 Thread Marco Lolicato
Hi Bill, First of all I would try to completely uninstall (via fink) both ccp4 and coot. In fact the problem is not the location of the programs per se, but the conflict between different versions of the same program. Fink can update the softwares, but not when you rebuild the whole system. Anyway,

[ccp4bb] Random coil protein

2011-12-04 Thread Marco Lolicato
Dear users, I'm just approaching to a new project...so, I have evidences of an interaction between the N-terminus region of a protein with its own C-terminal domain. The C-terminal domain is perfectly folded (easily expressed and purified), instead of the N-terminal one that is mostly random coi

Re: [ccp4bb] prescission protease cutting site

2011-08-02 Thread Marco Lolicato
Dear McCully, I always used this protease with a cleavage success near to 100% at 4°C over night without shaking. Concerning the construct, I putted the first Met just after the sequence LEVLFQ/GP SSP, so I added only three aminoacids after the cleavage site. All the best, Marco Il gio

Re: [ccp4bb] XDS question

2011-06-13 Thread Marco Lolicato
Thank you to all! @Frederic > > I have a problem with the following sentence: > "if I collect all spots I get good map, but it is impossible to solve the > structure by molecular replacement" - if you have a good map (I assume > electron density map) then the structure is solved... for me a goo

[ccp4bb] XDS question

2011-06-11 Thread Marco Lolicato
Dear all, I have a particular problem... so, I have a beautiful crystal with nice diffraction pattern at 2.7A. The diffraction images are composed by very strong spots and weak spots. With XDS, if I collect all spots I get good map, but it is impossible to solve the structure by molecular replace

[ccp4bb] AutoXDS

2011-03-02 Thread Marco Lolicato
Dear all, suggestion how to run autoxds on MAC OSX? I downloaded a script without success...do you know if are there others? Thanks, Marco