Dear PI s, and senior scientists' involved in recruitment,
Why do so many (especially postdoc) positions these days indicate:
> Readiness for high workload
> able to work independently but also effectively and collaboratively with
> other lab member
> Candidates should have a documented public
Dear Flemming,
On 8/21/19, Flemming Goery wrote:
> I find the message in my original e-mail has changed, perhaps by hackers,
What do u mean by 'hackers' BTW!!!
>
> Dear all:
> A has sought a job in the lab of B. B invited A for a interview with a PPT
> oral presentation, as requested A has sen
Dear all,
I have a structure where CYS is bound to OCA (octanoic acid). I
started with Jligand and created a CYS-OCA covalent link. It then
output a CIF file (verified looks OK). Add the LINK record to PDB
file:
LINK SG CYS A 161 C1 OCA A 902CYS-OCA
Place
Hi Graeme,
I suspect that this conclusions depends very closely on (i) the shape of
> the problem and (ii) the extent to which the binary has been optimised for
> the given platform.
>
> I do hope some of these info are analyzed and either published or at least
put at ccp4 wiki.
I am pretty sure
Hi Raquel,
Are u using a compressed filesystem? I recently moved everything including
/home directory to ZFS - which gave ~ 1.4X compression for old adsc
images. Remember vaguely, years before, James suggested to use
aufs/unionfs. You could even enable data-deduplication to save redundant
images.
Dear Pavel,
By coincidence today I was looking at a soak-dataset and got totally
confused with FEM (map) and Polder now.
I am working on a dataset that processed it at 2.7A (in summer with
XDS) and there was weak density for (OCA-Octanoic acid) when looked
with FEM. This week, I retried it with D
Dear all
Thanks to for all the responses. I would continue my question for
getting more advice.
I expressed a dimeric multi-protein (600 KDa). This protein was
purified (affinity chromatography) in 4 different common buffers and
run with the same SEC-buffer. We observe clear single size-exclusion
Dear all,
Not directly a ccp4 question.
I am working on a multi-domain protein with multiple catalytic
centres. Purifying in gel-filtration (Äkta) with different buffers
gives a clear distinct peak indicating pure protein. We could even
crystallise it and determine 3D structure to about 2.5 A.
Why
Dear all,
We are looking for a size exclusion chromatography column
(silica-based) for protein purification prior to a MALS-detector. We
looked for (www.waters.com BEH-450), Sepax (Unix-C 300) and Phenomex
(BioZen SEC-3). Any 'column' tips or recommendations when dealing
with large proteins (MDa)
Dear all,
we crystallized a small protein, that gives crystals P2 with cell
Cell 53.16 65.73 72.8990 110.94 90
(has 3 molecules in the asymmetric unit). Tested with pointless. Does
not give any other possibility.
The other crystal form of the same protein, similar conditions:
Dear all,
>From a small protein, gives crystals P2 with cell
Cell 53.16 65.73 72.8990 110.94 90
(has 3 molecules in the asymmetric unit). Tested with pointless. Does
not give any other possibility.
Another crystal if the same protein, similar conditions:
C2
Cell 109.14 1
If anyone from CCP4 website has noticed that...
Your connection is not secure
The owner of www.ccp4.ac.uk has configured their web site improperly.
To protect your information from being stolen, Firefox has not
connected to this web site.
(https warning message)
https://www.ccp4.ac.uk/ccp4onlin
Dear ccp4-ers,
I have a data set at 2.8 A. There is clear *continuous* density for a
ligand + residue. I used the LINK record to connect the S-gamma-atom
from Cys to C1-atom of ligand OCA. My biochemistry collaborator says
that CB-SG-C1-O1 should be approximately planar. However, during
refinement
Hi Sebastiano,
I posted the same question recently (in April). Just removed the names
and email ids for confidentiality (as some needs that).
SUMMARY -
I think biovia (aka accelrys) have a version, although I’ve never used
it, so I don’t know how good it is. Blair Johnston (Strathclyde)
Dear all,
This may be off-topic but this year our university has asked for
suggestions to move to a electronic lab notebook system. Are there any
good ones?
Due to data protection regulations they prefer hosted solutions. Many
of the hosted solutions I found by google are proprietary. I am
wonderin
Thanks for the responses. Yes, my crystal did survive and I can see
density for my ligand.
Summary:
1. If the crystal survives, it’s fine. These are just different ways
of exposing the crystal to the ligand and do what works. The only
issue will be if you don’t see electron density for the ligand
Dear all,
I wondered if it is OK to pipette ligand soln (X-CoA) *directly* to the
drop with crystal (1:1 ratio of protein:precipitant 2µl) instead of
dissolving it in precipitant solution and transferring the crystal to this
ligand containing precipitant solution. The crystals survive this as I add
17 matches
Mail list logo