[ccp4bb] Mysterious density

2010-07-09 Thread Nick Quade
Dear CCP4 community, I have solved the structure of a protein in complex with DNA. But, inside the protein there seems to be a ligand binding pocket with some strong density (*http://picasaweb.google.de/113264696790316881054/Desktop#). *The protein was in Tris buffer, with some NaCl, MgCl2 an

[ccp4bb] Detection of DNA in protein complex crystals

2009-06-23 Thread Nick Quade
Dear all, I am trying to solve the structure of a transcription factor in complex with its DNA. I got crystals of the complex under different conditions than the protein alone and they also look different. Unfortunately, they only diffract to 6Å so far. Before I continue to optimize the crystals