Dear all,
Sorry for off-topic question.
I want to study protein interaction with few small molecules using SPR
(Machine- *BIACORE 3000*).
The recombinant protein expressed in bacterial expression system is of 92
kDa (with His tag), pI= 9.
*Question-*
1. What should be the chip of choice- *NTA chi
Dear all,
I am graduate student working on a functional protein which i have cloned
in pET-28a vector for recombinant protein production in E.coli expression
system.
The expressed protein is purified on Ni-NTA resins with Imidazole gradient.
Surprisingly, i am getting distinct visible white precipi
