Re: [ccp4bb] suggestions for cryoprotectant

Hi Firdous, For salt conditions try malonate: Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2356-8. Epub 2003 Nov 27. Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. Holyoak T1, Fenn TD, Wilson MA, Moulin AG, Ringe D, Petsko GA. Be

Re: [ccp4bb] crystal size

Hi Vince, There is a very nice paper by Monika Budayova-Spano in Acta Cryst 63D 2007 339-347. "A methodology and an instrument for the temperature- controlled optimization of crystal growth." Best, Wally On Sep 10, 2008, at 2:23 AM, Vincenzo Carbone wrote: Dear all, I was wondering if an

Re: [ccp4bb] how to present the surface of a channel

Hi Jiamu, CASTp is a good tool for examining tunnels (http://sts-fw.bioengr.uic.edu/castp/ ). The UCSF program Chimera (http://www.cgl.ucsf.edu/chimera/) can download from the database and the latest snapshot can open your own CASTp calculations. Best, Wally On Aug 28, 2008, at 4:50 AM, J

Re: [ccp4bb] about anisotrophic diffraction

Hi Ji, Our lab has had good luck using sodium malonate to cryoprotect salt- grown crystals. See: Acta Cryst. (2003). D59, 2356-2358 Malonate: a versatile cryoprotectant and stabilizing solution for salt- grown macromolecular crystals T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ring

Re: [ccp4bb] Protein-protein docking

Hi Josiah, You could also try ClusPro. Very quick and easy. http://nrc.bu.edu/cluster/ Best, Wally On Feb 11, 2008, at 2:14 AM, [EMAIL PROTECTED] wrote: Hi all, Can anyone tell me what programs are available to do protein-protein docking? Thanks in advance, Regards, Josiah. Walter R.