Re: [ccp4bb] Hi

2015-05-28 Thread vijay srivastava
Dear All, I am able to do the superposition of the ligand with suplig.f program and superpose also do the same. Thank you for your valuable suggestions. regardsvijay  Vijay On Tuesday, 26 May 2015 6:11 PM, vijay srivastava wrote: Dear All, I want to superpose the nucleotide form

[ccp4bb] Hi

2015-05-26 Thread vijay srivastava
Dear All, I want to superpose the nucleotide form one GTPase on to the nucleotide of other GTPase in order to study the interaction in the nucleotide binding pocket. I tried to superpose but it is superposing on the basis of secondary structure as aresult both the nucleotides from two structutr

Re: [ccp4bb] Hi

2015-05-09 Thread vijay srivastava
Dear All, Sorry to disturb you all it can be easily done in ccp4mg. regards Vijay On Saturday, 9 May 2015 4:36 PM, vijay srivastava wrote: Dear All, In coot is there is any option so that we can change the colour of atoms. As in ball and stick model in default it is showing

[ccp4bb] Hi

2015-05-09 Thread vijay srivastava
Dear All, In coot is there is any option so that we can change the colour of atoms. As in ball and stick model in default it is showing sulphur as green. could we change the colour of atoms  to show carbon in gray, oxygen in red and suphur in yellow. regards Vijay

Re: [ccp4bb] hi - history of protein crystallization

2014-07-25 Thread vijay srivastava
Dear Dr. Sacha, it is giving following error after typing the command global: Command not found. wm: Command not found. set: Variable name must begin with a letter.   Vijay On Thursday, 24 July 2014 4:21 PM, Raghurama P Hegde wrote: Hi Sanjit,   These two reviews throw some light o

[ccp4bb] Hi

2014-07-20 Thread vijay srivastava
Dear All, Is there is any program to convert euler angles into theta, phi and kappa? regards   Vijay

Re: [ccp4bb] experimental phasing at low resolution

2014-06-03 Thread vijay srivastava
great for finding the sites, but at this resolution you want to refine the sites, and calculate phases with other programs/pipelines, like Phaser/Crank/Crunch. best, Kay On Tue, 3 Jun 2014 16:37:58 +0800, vijay srivastava wrote: >Dear All, Does anyone know of a facility that provides amino a

[ccp4bb] regarding cloning

2008-09-01 Thread vijay srivastava
Hi, I am trying to clone a 1.2kb insert into a expression vector pET 23a through T/A cloning.  The restriction enzyme used is Nhe1(NEB) and BamH1 (NEB) in the forward and reverse primer recpectively. I was succesful  in subcloning (T/A vector) and getting my insert at 1.2kb after  double digesti

[ccp4bb] hi

2008-08-30 Thread vijay srivastava
hi i am facing problem in cloning,getting my insert and vector at the correct position after digestion but after ligation colony is not coming and if how it is coming than i am not getting my insert   Add more friends to your messenger and enjoy! Go to http://in.messenger.yahoo.com/invi