Re: [ccp4bb] crystals that dont diffract :( :(

stem had a glitch, and there was no X-ray. True story! Best wishes -Z Zaigham Mahmood Khan, PhD Icahn School of Medicine at Mount Sinai Department of Oncological Sciences 1470 Madison Avenue New York On Tue, Aug 14, 2018 at 5:58 AM, Careina Edgooms < 02531c126adf-dmarc-requ...@jiscmail

Re: [ccp4bb] Unidentified electron density blob

s.. Best wishes -Z Zaigham Mahmood Khan, PhD Icahn School of Medicine at Mount Sinai Department of Oncological Sciences 1470 Madison Avenue New York On Fri, Jul 6, 2018 at 5:42 AM, Wim Burmeister wrote: > Hello, > a really molecule should show up also in the 2Fo-Fc type map. This appears

Re: [ccp4bb] Unidentified electron density blob

... Best wishes -Z Zaigham Mahmood Khan, PhD Icahn School of Medicine at Mount Sinai Department of Oncological Sciences 1470 Madison Avenue New York On Mon, Jul 2, 2018 at 10:23 PM, Uma Gabale < 0ebb5dcf3eaa-dmarc-requ...@jiscmail.ac.uk> wrote: > Dear all, > > We came a

Re: [ccp4bb] Fwd: Re: [ccp4bb] suggestion of crystallization optimization

hey Liu ... you got all wonderful suggestion, and they may be time consuming. Meantime, you may work on the crystals in hand, and follow the suggestions as mentioned above. >From my experience, i can tell you that crystal age is also an important parameter. I will shoot it as soon as i see it in

Re: [ccp4bb] how to improve the diffraction quality of the crystals and avoid icerings

d different kinds of cryoprotectants that have been successfully attempted in the past. Off-course, this also depends on type of precipitant that you used for crystallization. Apparently, this seems as glycerol worked better in your case, may be because you have salt-based precipitant. Best wishes

Re: [ccp4bb] Protein toxicity or low expression

Hey Firdous Yes, too much information can be confusing. I have expressed and purified over dozen of different proteins. I usually found in literature the protocol for the expression and purification of another homologous protein. That is the best way to start in my opinion. Some folks may want to

Re: [ccp4bb] PDB search help

sult section, and from pdb.org, you may find the residues of that protein that were observed in the electron density map as well as whole expression casstte that was attempted to crystallize for that particular pdb entry. Best wishes -Z Zaigham Mahmood Khan, PhD Icahn School of Medicin

Re: [ccp4bb] off topic

Hey Anamika I know SH2 domain is very much well-studied domain, and i am not sure why are you facing troubles in the expression of this protein. Just go through the literature and read about different protocol of expression of SH2 domain from several different proteins.. Well, reading that you hav

Re: [ccp4bb] Accidental crystallization of E. coli protein

This may seem an interesting case, given that protocol fur purification, and subsequent crystallization is reproducible as well as this enzyme is a valid drug target for pathogenic strains of E. coli. Best Z On Apr 5, 2017 02:51, "Vipul Panchal" wrote: > I don't think it is going to be any scien

Re: [ccp4bb] FW: [ccp4bb] Using a codon-optimised gene to improve protein solubility

! -Z Zaigham Mahmood Khan, PhD Icahn School of Medicine at Mount Sinai Department of Oncological Sciences 1470 Madison Avenue New York On Mon, Apr 3, 2017 at 6:30 AM, Noam Adir wrote: > Dear Supta, > > > > If I may make another type of suggestion, we have had success in > cryst

Re: [ccp4bb] Need suggestion for protein solubility

keeping protein concentration below 3 mg/ml solved the issue. Best wishes -Z Zaigham Mahmood Khan, PhD Fox Chase Cancer Center ICR R428 333 Cottman Avenue Philadelphia, 19111 PA Phone: 215 728 3609 On Sat, Dec 24, 2016 at 2:55 PM, Kevin Jin wrote: > > > Likely, the protein is pH

Re: [ccp4bb] MrBump and Balbes Molecular Replacement of multiple copies in ASU Failed even with high homology model

Yes, Eleanor D. is right. I had a similar experience. MR did not provide me the solution with monomeric protein. However, when i used dimer as a search model, it appeared to be a low-hanging fruit afterwards. May be you have a similar situation, there are very less possibilities to explain othewrwi