Darn it, you're right.
JPK
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes,
Jon
Sent: Thursday, March 30, 2017 4:05 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] AW: [ccp4bb] [ccp4bb] protein precipitation reg
yes, "oil of v
Apple products
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Mark
Wilson
Gesendet: Mittwoch, 29. März 2017 22:12
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] [ccp4bb] protein precipitation reg
I heartily concur with Craig. Tris can
IL.AC.UK
Betreff: Re: [ccp4bb] [ccp4bb] protein precipitation reg
Since I’m in full-on cranky old biochemist mode now, I think that vitriol is an
old name for sulfuric acid.
> On Mar 29, 2017, at 8:40 PM, Keller, Jacob wrote:
>
>> And if we are going to pour scorn and vitriol on Tri
Since I’m in full-on cranky old biochemist mode now, I think that vitriol is an
old name for sulfuric acid.
> On Mar 29, 2017, at 8:40 PM, Keller, Jacob wrote:
>
>> And if we are going to pour scorn and vitriol on Tris, why not mention its
>> large dpKa/dT of 0.03 pH units/deg ?
>
> Hah! That
>And if we are going to pour scorn and vitriol on Tris, why not mention its
>large dpKa/dT of 0.03 pH units/deg ?
Hah! That's what many people are doing when they make buffers: pouring vitriol
(HCl) on TRIS! I prefer to pour concentrated HEPES, and get two buffers without
adding any extra Cl-.
Behalf Of CRAIG A
BINGMAN
Sent: Thursday, 30 March 2017 8:54 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] [ccp4bb] protein precipitation reg
> On Mar 29, 2017, at 4:15 PM, Chun Luo wrote:
>
> In addition to price, the prevalence of Ni purification may be another reason
&
> On Mar 29, 2017, at 4:15 PM, Chun Luo wrote:
>
> In addition to price, the prevalence of Ni purification may be another reason
> for Tris popularity. Some His-tagged constructs don't bind to Ni well in
> HEPES. I wonder if anyone has similar experience or comments. —Chun
No, I have not spec
CP4BB@JISCMAIL.AC.UK] On Behalf Of Mark
Wilson
Sent: Wednesday, March 29, 2017 1:12 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] [ccp4bb] protein precipitation reg
I heartily concur with Craig. Tris can be a dangerous buffer for many reasons,
including those listed below. In addition, as a pr
I heartily concur with Craig. Tris can be a dangerous buffer for many
reasons, including those listed below. In addition, as a primary amine,
it can complicate work with metalloproteins and has moderate
nucleophilicity. There is almost always a better buffer choice than Tris.
Best regards,
Mark
There are almost always better choices than Tris buffer.
Mo Cleland used to call it “Trash” buffer. He is no longer with us, but today
I will happily carry that flag in his honor.
Tris may show up in your crystal structure, especially at carbohydrate binding
sites.
Tris may be a surprisingly s
10 matches
Mail list logo