oard [CCP4BB@JISCMAIL.AC.UK] On Behalf Of A K
[alek6...@gmail.com]
Sent: Tuesday, February 26, 2013 4:45 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Additives to bacterial cultures to improve solubility
Mark,
you can try low percentage of ethanol as well, as it increases the expression
of som
: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of A K
[alek6...@gmail.com]
Sent: Wednesday, 27 February 2013 9:45 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Additives to bacterial cultures to improve solubility
Mark,
you can try low percentage of ethanol as well, as it increases the
Mark,
you can try low percentage of ethanol as well, as it increases the
expression of some chaperones (can't find the paper right now!).
Alex
> On Tue, Feb 26, 2013 at 2:08 AM, Mark J van Raaij
> wrote:
>
>> Dear All,
>>
>> We are considering trying additives to bacterial cultures to see if the
Dear Mark,
I have had quite a lot of success by adding small molecules during cell
lysis to aid in solubility. From screening 100+ condition we found
trehalose, betaine and mannitol to be the standouts with aiding in
solubility.
Here is the relevant paper: http://www.ncbi.nlm.nih.gov/pubmed/23285
Dear Mark,
we have had a slight success increasing the solubility of some of our
proteins adding high concentration of salt (in addition to betaine and
glucose) to the culture media, as described in this paper
http://www.ncbi.nlm.nih.gov/pubmed/17126029
Sergio
2013/2/26 Mark J van Raaij
> Dea
Dear All,
We are considering trying additives to bacterial cultures to see if they
improve solubility of some proteins we express: L-arginine, glycyl-glycine,
sorbitol and betaine. I would be glad to receive recommendations and
experiences, also if they haven't worked.
Side-question: should we
