> They show a nice diffraction, but appear to be perfectly twinned.
In most cases, structures can be solved (and biological questions answered) in
twinned cases. I wouldn't be discouraged.
> I have crystallised a SeMet derivative, but I have not been able to collect
> sufficiently good data, to
Le 11/01/12 12:23, Federica Basilico a écrit :
> Hi everyone,
>
> I have crystals of a protein of 176 residues, with 5 Cys, 8 His, 3 Met.
> Native crystals grow in 10% MPD, 100mM Bicine pH 9.0. They show a nice
> diffraction, but appear to be perfectly twinned.
> I have crystallised a SeMet deriva
Hi everyone,
I have crystals of a protein of 176 residues, with 5 Cys, 8 His, 3 Met.
Native crystals grow in 10% MPD, 100mM Bicine pH 9.0. They show a nice
diffraction, but appear to be perfectly twinned.
I have crystallised a SeMet derivative, but I have not been able to
collect sufficiently g