Re: [ccp4bb] Choice of heavy atom derivatives

2012-01-11 Thread Francis E Reyes
> They show a nice diffraction, but appear to be perfectly twinned. In most cases, structures can be solved (and biological questions answered) in twinned cases. I wouldn't be discouraged. > I have crystallised a SeMet derivative, but I have not been able to collect > sufficiently good data, to

Re: [ccp4bb] Choice of heavy atom derivatives

2012-01-11 Thread Miguel Ortiz Lombardía
Le 11/01/12 12:23, Federica Basilico a écrit : > Hi everyone, > > I have crystals of a protein of 176 residues, with 5 Cys, 8 His, 3 Met. > Native crystals grow in 10% MPD, 100mM Bicine pH 9.0. They show a nice > diffraction, but appear to be perfectly twinned. > I have crystallised a SeMet deriva

[ccp4bb] Choice of heavy atom derivatives

2012-01-11 Thread Federica Basilico
Hi everyone, I have crystals of a protein of 176 residues, with 5 Cys, 8 His, 3 Met. Native crystals grow in 10% MPD, 100mM Bicine pH 9.0. They show a nice diffraction, but appear to be perfectly twinned. I have crystallised a SeMet derivative, but I have not been able to collect sufficiently g