Or you could just bug me to find the PNGaseF expression plasmid that I should
have in the freezer somewhere!
LOO T., PATCHETT M. L., NORRIS G. E. & LOTT J. S. “Using Secretion to Solve a
Solubility Problem: High-yield Expression in E. coli and Purification of the
Bacterial Glycoamidase PNGase F
Original message
>Date: Fri, 18 Jun 2010 17:10:55 +1000
>From: CCP4 bulletin board (on behalf of Aaron Oakley
>)
>Subject: [ccp4bb] Deglycosylation enzymes
>To: CCP4BB@JISCMAIL.AC.UK
>
>Hi all,
>
>I am looking to obtain plasmids encoding the deglycosylating enzy
Hi all,
I am looking to obtain plasmids encoding the deglycosylating enzymes
peptide-N-glycosidase F and endoglycosidase F1
for expression as fusion proteins with GST. They were described in the
following paper:
Deglycosylation of proteins for crystallization using recombinant fusion
protein g
