ober 4, 2019 5:06:10 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
Thanks to All for the extended & informative responses. If true thermodynamic
equilibria
are realized, then I would agree that regardless of the pathway the endpoint
(or integrated H)
sho
m. I may keep bugging selected victims off-board once I
have the first data.
Many thanks again, BR
From: CCP4 bulletin board On Behalf Of Barone,
Matthias
Sent: Thursday, October 3, 2019 18:59
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
As Reza alr
"Bernhard Rupp"
À: CCP4BB@JISCMAIL.AC.UK
Envoyé: Jeudi 3 Octobre 2019 17:05:56
Objet: [ccp4bb] ITC question -dimer vs monomer
Hi Fellows,
please let me ask the respective experts an ITC question: I have 2 proteins,
stable and dialyzed in identical buffer.
A is a monomer and B an obli
93-284
From: CCP4 bulletin board on behalf of Roger Rowlett
Sent: Thursday, October 3, 2019 8:36:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
Won't this depend on the relative final concentrations of A and B in the two
experiments? If A
nt:* Thursday, October 3, 2019 17:41
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] ITC question -dimer vs monomer
>
>
>
> I don’t understand what you are trying to do—are you trying to show, by
> the difference in ITC response, that th
so try with SPR. Always best to do these things
> using multiple complimentary methods
>
>
>
> Best wishes
>
>
>
> Clare
>
>
>
> *From:* CCP4 bulletin board *On Behalf Of *Bernhard
> Rupp
> *Sent:* 03 October 2019 16:06
> *To:* CCP4BB@JISCMAIL.AC.UK
mp;source=web&rct=j&url=http://www.chem.gla.ac.uk/staff/alanc/itcdil.pdf&ved=2ahUKEwiOpoeisYDlAhUDThUIHX5mAyMQFjAAegQIAhAB&usg=AOvVaw0Ng-W03upy4DG12unFGDY3
From: CCP4 bulletin board On Behalf Of Bernhard Rupp
Sent: 03 October 2019 16:52
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4
2019 16:06
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ITC question -dimer vs monomer
Hi Fellows,
please let me ask the respective experts an ITC question: I have 2 proteins,
stable and dialyzed in identical buffer.
A is a monomer and B an obligate dimer. I suspect that eventually a A2B2 dimer
em.gla.ac.uk/staff/alanc/itcdil.pdf&ved=2ahUKEwiOpoeisYDlAhUDThUIHX5mAyMQFjAAegQIAhAB&usg=AOvVaw0Ng-W03upy4DG12unFGDY3
From: CCP4 bulletin board On Behalf Of Bernhard Rupp
Sent: 03 October 2019 16:52
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
I am not
College of New York
Department of Chemistry
New York, NY 10031
From: CCP4 bulletin board on behalf of Bernhard Rupp
Sent: Thursday, October 3, 2019 11:51 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] Re: [ccp4bb] ITC question -dimer vs monomer
I am not looki
: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
I don't understand what you are trying to do-are you trying to show, by the
difference in ITC response, that the predictions you made about the
oligomerization are true?
Sent: Thursday, October 3, 2019 11:06 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ITC question -dimer vs monomer
Hi Fellows,
please let me ask the respective experts an ITC question: I have 2 proteins,
stable and dialyzed in identical buffer.
A is a monomer and B an obligate dimer. I suspect
Hi Fellows,
please let me ask the respective experts an ITC question: I have 2 proteins,
stable and dialyzed in identical buffer.
A is a monomer and B an obligate dimer. I suspect that eventually a A2B2
dimer will form.
Intuitively, it should make a difference whether I titrate the dimer w
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