Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-02-02 Thread Jose Brandao-Neto
Hanging or sitting drops, it's there any other reason you're looking for different cover slides? There are hydrophilic/phobic finishings to pick from, plus oil is also an alternative both to treat the surface or to screen in oil (micro)batch. Plus if hanging is your thing, dispense on a sittin

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-02-01 Thread Uma Gabale
We used to use 1% solution of dimethyl dichloro silane in toluene to siliconize glass coverslips. Soak them for 30 min, rinse well with deioinized water, and oven/air dry.Uma. --Uma Gabale, PhDResearch AssociateMolecular and Cellular Biochemistry Indiana University Bloomington On Thursday,

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Minmin Yu
@JISCMAIL.AC.UKReply To: Frank Von DelftSubject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Or do sitting drop, it's much easier all round. Frank Sent from tiny silly touch screen From: Minmin Yu Sent: Thursday, 31 January 2019 21:42 To: C

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Frank Von Delft
Or do sitting drop, it's much easier all round. Frank Sent from tiny silly touch screen From: Minmin Yu Sent: Thursday, 31 January 2019 21:42 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Minmin Yu
Yeah I used to use Fisher Scientific square plastic cover slips. Now I am using Sigma 22x22mm (Z36,590-4, 100 each Lot10B084279) smaller sized ones. These plastic ones are much more durable than the glass ones and are very easy to be handled. They work very well for both hanging drops or for sit

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Daniel M. Himmel, Ph. D.
Fisher Scientific makes 22 inch square plastic cover slips, which are labeled "Fisherbrand Unbreakable Cover Slips". I and my colleagues have used them for over 15 years instead of siliconized glass coverslips. Just dip each one in ethanol to clean before use, dry with an air blower, and use it to

[ccp4bb] AW: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Hughes, Jon
alternative to siliconized glass coverslips for crystallization? When I was a graduate student, about 150,000 years ago, we took regular coverslips and doused them in ?silane to make siliconised ones. You then let them sit in a rack to dry. It was a bit tedious but not horrendously so. After a

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Patrick Shaw Stewart
nal Message- > > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of > Goldman, Adrian > > Sent: Thursday, January 31, 2019 8:43 AM > > To: CCP4BB@JISCMAIL.AC.UK > > Subject: Re: [ccp4bb] Is there any alternative to siliconized glass > coverslips fo

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Goldman, Adrian
--Original Message- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of > Goldman, Adrian > Sent: Thursday, January 31, 2019 8:43 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Is there any alternative to siliconized glass > coverslips for crystalliz

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Mathews, Irimpan I.
there any alternative to siliconized glass coverslips for crystallization? When I was a graduate student, about 150,000 years ago, we took regular coverslips and doused them in ?silane to make siliconised ones. You then let them sit in a rack to dry. It was a bit tedious but not horrendously so

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Goldman, Adrian
When I was a graduate student, about 150,000 years ago, we took regular coverslips and doused them in ?silane to make siliconised ones. You then let them sit in a rack to dry. It was a bit tedious but not horrendously so. After a while, I stopped doing it altogether, because IMHO it didn’t ma

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Holton, James M
plastic. Plastic cover slips are no good for UV or polarization, but they are way better than glass if you happen to want to try in-situ diffraction. (https://doi.org/10.1107/S002188981254) If you can't afford commercial ones, then you can always cut up some inkjet transparency film sheets

Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Mitchell D. Miller
[ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a

[ccp4bb] AW: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Hughes, Jon
: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? I know of Rain-X being used. V. Nagarajan On Thu, Jan 31, 2019 at 1:17 AM mailto:herman.schreu...@sanofi.com>> wrote: A long time ago, before siliconized coverslips became commer

Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Nagarajan V
> *Betreff:* [EXTERNAL] [ccp4bb] Is there any alternative to siliconized > glass coverslips for crystallization? > > > > Dear All > > I am a PhD student who requires lots of coverslips (!!) for setting up > hanging drop crystallization. The company sells it for a

Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Zhijie Li
[ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal

Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Tjaard Pijning
board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of herman.schreu...@sanofi.com Sent: 31 januari 2019 10:17 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? A long time ago, before siliconized coverslips

[ccp4bb] AW: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Herman . Schreuder
board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Rajnandani Kashyap Gesendet: Donnerstag, 31. Januar 2019 09:17 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization? Dear All I am a PhD student who requires lots of

[ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

2019-01-31 Thread Rajnandani Kashyap
Dear All I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal siliconized coverslip and a 22mm siliconized circle coverslips. We tried to search for an a