Re: [ccp4bb] Off-topic: DSF thermo cycler low temp limit

2011-10-27 Thread Morten Groftehauge
Lots of systems go to 4C. Even a cheapo one like the Applied Biosystems StepOnePlus. -Morten Groftehauge On 27 October 2011 03:17, Jürgen Bosch wrote: > CD spec with Pelletier is an option too > > Jürgen > > .. > Jürgen Bosch > Johns Hopkins Bloomberg School of Public Health

Re: [ccp4bb] Off-topic: DSF thermo cycler low temp limit

2011-10-26 Thread Jürgen Bosch
CD spec with Pelletier is an option too Jürgen .. Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab:

[ccp4bb] Off-topic: DSF thermo cycler low temp limit

2011-10-26 Thread Reginald McNulty
We are trying to determine Tm value of rather unstable proteins with a Tm in the mid 20 C range using DSF/thermofluor. Our Stratagene thermocycler has a low temp limit of 25 C (Peltier). I called the company and they said it's a 'hardware limit' that cannot be changed. 1) Has anyone been able to