.AC.UK] on behalf of Linda Olson
[lol...@mcw.edu]
Sent: 26 December 2012 18:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] SeCys usage for SAD
Dear All,
I have read a recent paper by Salgado et al about using non-auxotrophic E.coli
to incorporate SeCys into recombinant protein for phasing purposes
You might try mercury soaks instead. Might give you 15 minute answers to your
questions.
HNY!
Ho
Sent from my iPad
On Dec 26, 2012, at 4:00 PM, CCP4BB automatic digest system
wrote:
> There is 1 message totaling 34 lines in this issue.
>
> Topics of the day:
>
> 1. SeCys usage for SAD
>
Dear Linda, If your molecule is not very big, your cell is not very big, you
have an approach to modern in-house X-ray facility, your crystal diffract
reasonably well ( :-\ ) you can try S phasing. If you will be able to measure
accurate data, S anomalous phasing at 1.5417 Angstrom of Cu radiati
Dear All,
I have read a recent paper by Salgado et al about using non-auxotrophic E.coli
to incorporate SeCys into recombinant protein for phasing purposes. Does
anyone have a source for Selenocysteine? I have also seen a paper by Schaefer
et al which uses nitric acid treated elemental Se for
