AC.UK] on behalf of James Holton
[jmhol...@lbl.gov]
Sent: 07 July 2014 00:59
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Selenomethionine crystals
I general I agree with Tim, but provided it doesn't kill your protein,
oxidation of selenomethionine can actually enhance your anomal
I general I agree with Tim, but provided it doesn't kill your protein,
oxidation of selenomethionine can actually enhance your anomalous signal
considerably (http://dx.doi.org/10.1107/S0907444901008666).
The reason for this is because the "white line" peak in the SeMet x-ray
absorption spectru
Dear Maher,
as far as I understand, the anomalous scattering comes from inner shell
electrons, not the valence electrons. So while you might notice a slight shift
in the peak wavelength, the strength of the signal will only reduce if you
crystal order suffered over time, but not from any oxidation
Hi everyone,
Would anyone know for how long Selenomethionine derivative crystals are
good if kept in plate at RT. In other words, would SE loose its scattering
properties due to oxidation over time? I have SElmet crystals that have
been lying in a plate for 2 months by now so I was wondering if th
