Re: [ccp4bb] Skin on the drop

2016-11-17 Thread Mark J van Raaij
Microbatch under oil in Terasaki plates or perhaps microdialysis might be an option. Then you don’t have a liquid/air interface. It is harder to do very small volumes then, especially with microdialysis. Or free interface diffusion in thin tubes (or with specialised equipment). Mark J van Raaij

[ccp4bb] Skin on the drop

2016-11-17 Thread xiaoron...@cau.edu.cn
Dear All, How to avoid the skin on the drop? These skins are believed to be a layer of denatured protein or caused by the polyethylene glycol. They will certainly slow down the rate of vapor diffusion. If you have any good suggestions to avoid skin forming? Best wishes, Xiaorong Li xiaor